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Journal of Bacteriology logoLink to Journal of Bacteriology
. 1985 May;162(2):579–583. doi: 10.1128/jb.162.2.579-583.1985

alpha-Aminoadipate as a primary nitrogen source for Saccharomyces cerevisiae mutants.

K S Zaret, F Sherman
PMCID: PMC218887  PMID: 3921525

Abstract

In contrast to wild-type strains of the yeast Saccharomyces cerevisiae, lys2 and lys5 mutants are able to utilize alpha-aminoadipate as a primary source of nitrogen. Chattoo et al. (B. B. Chattoo, F. Sherman, D. A. Azubalis, T. A. Fjellstedt, D. Mehnert, and M. Ogur, Genetics 93:51-65, 1979) relied on this difference in the effective utilization of alpha-aminoadipate to develop a procedure for directly selecting lys2 and lys5 mutants. In this study we used a range of mutant strains and various media to determine why normal strains are unable to utilize alpha-aminoadipate as a nitrogen source. Our results demonstrate that the anabolism of high levels of alpha-aminoadipate through the biosynthetic pathway of lysine results in the accumulation of a toxic intermediate and, furthermore, that lys2 and lys5 mutants contain blocks leading to the formation of this intermediate.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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