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. 1985 May;162(2):584–590. doi: 10.1128/jb.162.2.584-590.1985

DNA transfer occurs during a cell surface contact stage of F sex factor-mediated bacterial conjugation.

M M Panicker, E G Minkley Jr
PMCID: PMC218888  PMID: 2859268

Abstract

Donor bacteria containing JCFL39, a temperature-sensitive traD mutant of the F sex factor, were used at the nonpermissive temperature to accumulate stable mating pairs with recipient cells. At this stage in conjugation, extracellular F pili were removed by treatment with 0.01% sodium dodecyl sulfate. Upon then shifting to the permissive temperature for JCFL39, transfer of the F plasmid was observed. The mating pairs that were accumulated with JCFL39 at the nonpermissive temperature were readily observed by electron microscopy in wall-to-wall contact with the recipient bacteria. These results demonstrate that the traD product, which is known to be required in transferring DNA to a recipient bacterium, acts after the stage at which extracellular F pili are required. In addition, we concluded that DNA transfer takes place while donor and recipient cells are in surface contact and not necessarily through an extended F pilus as envisioned in some models of bacterial conjugation.

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Selected References

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  1. ANDERSON T. F. Recombination and segregation in Escherichia coli. Cold Spring Harb Symp Quant Biol. 1958;23:47–58. doi: 10.1101/sqb.1958.023.01.007. [DOI] [PubMed] [Google Scholar]
  2. Abdel-Monem M., Taucher-Scholz G., Klinkert M. Q. Identification of Escherichia coli DNA helicase I as the traI gene product of the F sex factor. Proc Natl Acad Sci U S A. 1983 Aug;80(15):4659–4663. doi: 10.1073/pnas.80.15.4659. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Achtman M. Genetics of the F sex factor in enterobacteriaceae. Curr Top Microbiol Immunol. 1973;60:79–123. doi: 10.1007/978-3-642-65502-9_3. [DOI] [PubMed] [Google Scholar]
  4. Achtman M., Morelli G., Schwuchow S. Cell-cell interactions in conjugating Escherichia coli: role of F pili and fate of mating aggregates. J Bacteriol. 1978 Sep;135(3):1053–1061. doi: 10.1128/jb.135.3.1053-1061.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Achtman M., Willetts N., Clark A. J. Beginning a genetic analysis of conjugational transfer determined by the F factor in Escherichia coli by isolation and characterization of transfer-deficient mutants. J Bacteriol. 1971 May;106(2):529–538. doi: 10.1128/jb.106.2.529-538.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. BRINTON C. C., Jr, GEMSKI P., Jr, CARNAHAN J. A NEW TYPE OF BACTERIAL PILUS GENETICALLY CONTROLLED BY THE FERTILITY FACTOR OF E. COLI K 12 AND ITS ROLE IN CHROMOSOME TRANSFER. Proc Natl Acad Sci U S A. 1964 Sep;52:776–783. doi: 10.1073/pnas.52.3.776. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Brinton C. C., Jr The properties of sex pili, the viral nature of "conjugal" genetic transfer systems, and some possible approaches to the control of bacterial drug resistance. CRC Crit Rev Microbiol. 1971 May;1(1):105–160. doi: 10.3109/10408417109104479. [DOI] [PubMed] [Google Scholar]
  8. Burnette W. N. "Western blotting": electrophoretic transfer of proteins from sodium dodecyl sulfate--polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A. Anal Biochem. 1981 Apr;112(2):195–203. doi: 10.1016/0003-2697(81)90281-5. [DOI] [PubMed] [Google Scholar]
  9. Curtiss R., 3rd Bacterial conjugation. Annu Rev Microbiol. 1969;23:69–136. doi: 10.1146/annurev.mi.23.100169.000441. [DOI] [PubMed] [Google Scholar]
  10. Hane M. W. Some effects of nalidixic acid on conjugation in Escherichia coli K-12. J Bacteriol. 1971 Jan;105(1):46–56. doi: 10.1128/jb.105.1.46-56.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Jarvik J., Botstein D. A genetic method for determining the order of events in a biological pathway. Proc Natl Acad Sci U S A. 1973 Jul;70(7):2046–2050. doi: 10.1073/pnas.70.7.2046. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kennedy N., Beutin L., Achtman M., Skurray R., Rahmsdorf U., Herrlich P. Conjugation proteins encoded by the F sex factor. Nature. 1977 Dec 15;270(5638):580–585. doi: 10.1038/270580a0. [DOI] [PubMed] [Google Scholar]
  13. Kingsman A., Willetts N. The requirements for conjugal DNA synthesis in the donor strain during flac transfer. J Mol Biol. 1978 Jul 5;122(3):287–300. doi: 10.1016/0022-2836(78)90191-2. [DOI] [PubMed] [Google Scholar]
  14. LEDERBERG J. Conjugal pairing in Escherichia coli. J Bacteriol. 1956 Apr;71(4):497–498. doi: 10.1128/jb.71.4.497-498.1956. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Manning P. A., Morelli G., Achtman M. traG protein of the F sex factor of Escherichia coli K-12 and its role in conjugation. Proc Natl Acad Sci U S A. 1981 Dec;78(12):7487–7491. doi: 10.1073/pnas.78.12.7487. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Marvin D. A., Hohn B. Filamentous bacterial viruses. Bacteriol Rev. 1969 Jun;33(2):172–209. doi: 10.1128/br.33.2.172-209.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Minkley E. G., Jr Purification and characterization of pro-TraTp, the signal sequence-containing precursor of a secreted protein encoded by the F sex factor. J Bacteriol. 1984 May;158(2):464–473. doi: 10.1128/jb.158.2.464-473.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Skurray R. A., Hancock R. E., Reeves P. Con--mutants: class of mutants in Escherichia coli K-12 lacking a major cell wall protein and defective in conjugation and adsorption of a bacteriophage. J Bacteriol. 1974 Sep;119(3):726–735. doi: 10.1128/jb.119.3.726-735.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Sugino A., Peebles C. L., Kreuzer K. N., Cozzarelli N. R. Mechanism of action of nalidixic acid: purification of Escherichia coli nalA gene product and its relationship to DNA gyrase and a novel nicking-closing enzyme. Proc Natl Acad Sci U S A. 1977 Nov;74(11):4767–4771. doi: 10.1073/pnas.74.11.4767. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. TAYLOR A. L., THOMAN M. S. THE GENETIC MAP OF ESCHERICHIA COLI K-12. Genetics. 1964 Oct;50:659–677. doi: 10.1093/genetics/50.4.659. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Tomoeda M., Inuzuka M., Date T. Bacterial sex pili. Prog Biophys Mol Biol. 1975;30(1):23–56. doi: 10.1016/0079-6107(76)90004-3. [DOI] [PubMed] [Google Scholar]
  22. Willetts N., Achtman M. Genetic analysis of transfer by the Escherichia coli sex factor F, using P1 transductional complementation. J Bacteriol. 1972 Jun;110(3):843–851. doi: 10.1128/jb.110.3.843-851.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Willetts N., Skurray R. The conjugation system of F-like plasmids. Annu Rev Genet. 1980;14:41–76. doi: 10.1146/annurev.ge.14.120180.000353. [DOI] [PubMed] [Google Scholar]
  24. Willetts N., Wilkins B. Processing of plasmid DNA during bacterial conjugation. Microbiol Rev. 1984 Mar;48(1):24–41. doi: 10.1128/mr.48.1.24-41.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]

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