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. 1985 May;162(2):611–614. doi: 10.1128/jb.162.2.611-614.1985

Detection of lipoglycans in ureaplasmas.

P F Smith
PMCID: PMC218892  PMID: 3988705

Abstract

Serotypes 3, 4, and 8 of Ureaplasma urealyticum were found to contain lipoglycans. Although the ratios of their components differed, all contained neutral sugars, fatty acids, glycerol, and phosphorus. All three became labeled when the organisms were cultivated in the presence of [14C]glucose, [14C]palmitic or [14C]oleic acids, and inorganic 32P. Only neutral sugars were found, and these consisted of mannose, glucose, and galactose. Hot phenol extracts of uninoculated and supernatant culture media contained polymeric carbohydrate, but this differed in composition from ureaplasmal lipoglycans and did not become radiolabeled. Since lipoglycans contained phosphorus but no amino sugars, they could be separated from contaminating polysaccharides by anion exchange chromatography.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Al-Samarrai T. H., Smith P. F., Lynn R. J. Identification of the major antigenic determinants on lipoglycans from Acholeplasma granularum and Acholeplasma axanthum. Infect Immun. 1983 May;40(2):629–632. doi: 10.1128/iai.40.2.629-632.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Al-Samarrai T. H., Smith P. F., Lynn R. J. Isolation and characterization of the sheep erythrocyte receptor for acholeplasmal lipoglycans. Infect Immun. 1982 Dec;38(3):1078–1087. doi: 10.1128/iai.38.3.1078-1087.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Al-Shammari A. J., Smith P. F. Lipid and lipopolysaccharide composition of Acholeplasma oculi. J Bacteriol. 1979 Aug;139(2):356–361. doi: 10.1128/jb.139.2.356-361.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Ballou C. E., Raschke W. C. Polymorphism of the somatic antigen of yeast. Science. 1974 Apr 12;184(4133):127–134. doi: 10.1126/science.184.4133.127. [DOI] [PubMed] [Google Scholar]
  5. Green F., 3rd, Hanson R. P. Ultrastructure and capsule of Mycoplasma meleagridis. J Bacteriol. 1973 Nov;116(2):1011–1018. doi: 10.1128/jb.116.2.1011-1018.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Howard C. J., Gourley R. N. An electron-microscopic examination of certain bovine mycoplasmas stained with ruthenium red and the demonstration of a capsule on Mycoplasma dispar. J Gen Microbiol. 1974 Aug;83(2):393–398. doi: 10.1099/00221287-83-2-393. [DOI] [PubMed] [Google Scholar]
  7. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  8. Lynn R. J., Guidici P. A., Hollemweguer E. J., Smith P. F. Humoral immune response of rabbits to acholeplasmal lipoglycans. Infect Immun. 1980 Sep;29(3):926–933. doi: 10.1128/iai.29.3.926-933.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Manchee R. J., Taylor-Robinson D. Enhanced growth of T-strain mycoplasmas with N-2-hydroxyethylpiperazone-N'-2-ethanesulfonic acid buffer. J Bacteriol. 1969 Oct;100(1):78–85. doi: 10.1128/jb.100.1.78-85.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Mayberry W. R., Smith P. F., Langworthy T. A. Heptose-containing pentaglycosyl diglyceride among the lipids of Acholeplasma modicum. J Bacteriol. 1974 Jun;118(3):898–904. doi: 10.1128/jb.118.3.898-904.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Plackett P., Buttery S. H. A galactofuranose disaccharide from the galactan of Mycoplasma mycoides. Biochem J. 1964 Jan;90(1):201–205. doi: 10.1042/bj0900201. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Robertson J., Smook E. Cytochemical evidence of extramembranous carbohydrates on Ureaplasma urealyticum (T-strain Mycoplasma). J Bacteriol. 1976 Nov;128(2):658–660. doi: 10.1128/jb.128.2.658-660.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Seid R. C., Jr, Smith P. F., Guevarra G., Hochstein H. D., Barile M. F. Endotoxin-like activities of mycoplasmal lipopolysaccharides (lipoglycans). Infect Immun. 1980 Sep;29(3):990–994. doi: 10.1128/iai.29.3.990-994.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Smith P. F., Langworthy T. A., Mayberry W. R. Distribution and composition of lipopolysaccharides from mycoplasmas. J Bacteriol. 1976 Mar;125(3):916–922. doi: 10.1128/jb.125.3.916-922.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Smith P. F. Sequence and glycosidic bond arrangement of sugars in lipopolysaccharide from Thermoplasma acidophilum. Biochim Biophys Acta. 1980 Aug 11;619(2):367–373. doi: 10.1016/0005-2760(80)90084-3. [DOI] [PubMed] [Google Scholar]
  16. Smith P. F. Structure of the oligosaccharide chain of lipoglycan from Acholeplasma granularum. Biochim Biophys Acta. 1981 Jul 24;665(1):92–99. doi: 10.1016/0005-2760(81)90237-x. [DOI] [PubMed] [Google Scholar]
  17. Whitescarver J., Castillo F., Furness G. The preparation of membranes of some human T-mycoplasmas and the analysis of their carbohydrate content. Proc Soc Exp Biol Med. 1975 Oct;150(1):20–22. doi: 10.3181/00379727-150-38965. [DOI] [PubMed] [Google Scholar]

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