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. 1985 May;162(2):656–660. doi: 10.1128/jb.162.2.656-660.1985

Comparison of 10 IncP plasmids: homology in the regions involved in plasmid replication.

G K Chikami, D G Guiney, T J Schmidhauser, D R Helinski
PMCID: PMC218900  PMID: 2985542

Abstract

We have examined the DNA homology in the replication regions of 10 IncP plasmids independently isolated from several different countries. Two regions of RK2, the best-studied plasmid of this group, are required for vegetative DNA replication: the origin of replication (oriV) and the trfA region, which codes for a gene product necessary for replication. Six of nine IncP plasmids studied were identical to RK2 in the oriV and trfA regions as shown by Southern hybridization. Three P plasmids, R751, R772, and R906, showed weaker homology with the RK2 trfA, region and hybridized to different-sized HaeII fragments than the other six plasmids. R751, R772, and R906 hybridized to the region of the RK2 replication origin which expresses P incompatibility but differed markedly from RK2 and the other six plasmids in the GC-rich region of the origin required for replication. These data indicate that the P-group plasmids can be divided into two subgroups: IncP alpha, which includes the RK2-like plasmids, and IncP beta which includes the R751-like plasmids.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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