Skip to main content
PMC home page
The Journal of Experimental Medicine logoLink to The Journal of Experimental Medicine
. 1988 Aug 1;168(2):463–474. doi: 10.1084/jem.168.2.463

Interleukin 1 enhances growth factor-dependent proliferation of the clonogenic cells in acute myeloblastic leukemia and of normal human primitive hemopoietic precursors

PMCID: PMC2189011  PMID: 3261773

Abstract

IL-1 is released by activated monocytes and is thought to be a key mediator of the host immune response. The availability of the purified and, more recently, recombinant IL-1 has allowed the characterization of other biological properties of this molecule. Thus, IL-1 is thought to have the same properties as hemopoietic 1, a growth factor that has been shown to act on primitive murine hemopoietic cells. Here we report that rIL-1 acts synergistically with granulocyte/macrophage CSF (GM- CSF) or granulocyte CSF in the stimulation of clonogenic cells from many patients with acute myeloblastic leukemia (AML). Although IL-1 by itself has no effect on AML blasts, it can support colony formation under conditions where there is detectable production of endogenous GM- CSF. IL-1 also promotes the growth of multipotential progenitors from normal human bone marrow cells in the presence of GM-CSF. These observations support the hypothesis that in the hemopoietic system, IL- 1 has a selective effect on primitive precursors.

Full Text

The Full Text of this article is available as a PDF (715.3 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Dower S. K., Kronheim S. R., Hopp T. P., Cantrell M., Deeley M., Gillis S., Henney C. S., Urdal D. L. The cell surface receptors for interleukin-1 alpha and interleukin-1 beta are identical. Nature. 1986 Nov 20;324(6094):266–268. doi: 10.1038/324266a0. [DOI] [PubMed] [Google Scholar]
  2. Duff G. Immune diseases. Many roles for interleukin-1. 1985 Jan 31-Feb 6Nature. 313(6001):352–353. doi: 10.1038/313352a0. [DOI] [PubMed] [Google Scholar]
  3. Feinberg A. P., Vogelstein B. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem. 1983 Jul 1;132(1):6–13. doi: 10.1016/0003-2697(83)90418-9. [DOI] [PubMed] [Google Scholar]
  4. Fibbe W. E., van Damme J., Billiau A., Voogt P. J., Duinkerken N., Kluck P. M., Falkenburg J. H. Interleukin-1 (22-K factor) induces release of granulocyte-macrophage colony-stimulating activity from human mononuclear phagocytes. Blood. 1986 Dec;68(6):1316–1321. [PubMed] [Google Scholar]
  5. Gabrilove J. L., Welte K., Harris P., Platzer E., Lu L., Levi E., Mertelsmann R., Moore M. A. Pluripoietin alpha: a second human hematopoietic colony-stimulating factor produced by the human bladder carcinoma cell line 5637. Proc Natl Acad Sci U S A. 1986 Apr;83(8):2478–2482. doi: 10.1073/pnas.83.8.2478. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Griffin J. D., Löwenberg B. Clonogenic cells in acute myeloblastic leukemia. Blood. 1986 Dec;68(6):1185–1195. [PubMed] [Google Scholar]
  7. Hoang T., Iscove N. N., Odartchenko N. Macromolecules stimulating human granulocytic colony-forming cells, precursors of these cells, and primitive erythroid progenitors: some apparent nonidentities. Blood. 1983 May;61(5):960–966. [PubMed] [Google Scholar]
  8. Hoang T., McCulloch E. A. Production of leukemic blast growth factor by a human bladder carcinoma cell line. Blood. 1985 Sep;66(3):748–751. [PubMed] [Google Scholar]
  9. Jubinsky P. T., Stanley E. R. Purification of hemopoietin 1: a multilineage hemopoietic growth factor. Proc Natl Acad Sci U S A. 1985 May;82(9):2764–2768. doi: 10.1073/pnas.82.9.2764. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kelleher C., Miyauchi J., Wong G., Clark S., Minden M. D., McCulloch E. A. Synergism between recombinant growth factors, GM-CSF and G-CSF, acting on the blast cells of acute myeloblastic leukemia. Blood. 1987 May;69(5):1498–1503. [PubMed] [Google Scholar]
  11. Kriegler A. B., Bradley T. R., Januszewicz E., Hodgson G. S., Elms E. F. Partial purification and characterization of a growth factor for macrophage progenitor cells with high proliferative potential in mouse bone marrow. Blood. 1982 Aug;60(2):503–508. [PubMed] [Google Scholar]
  12. Larsson E. L., Iscove N. N., Coutinho A. Two distinct factors are required for induction of T-cell growth. Nature. 1980 Feb 14;283(5748):664–666. doi: 10.1038/283664a0. [DOI] [PubMed] [Google Scholar]
  13. March C. J., Mosley B., Larsen A., Cerretti D. P., Braedt G., Price V., Gillis S., Henney C. S., Kronheim S. R., Grabstein K. Cloning, sequence and expression of two distinct human interleukin-1 complementary DNAs. Nature. 1985 Jun 20;315(6021):641–647. doi: 10.1038/315641a0. [DOI] [PubMed] [Google Scholar]
  14. McCandliss R., Sloma A., Pestka S. Isolation and cell-free translation of human interferon mRNA from fibroblasts and leukocytes. Methods Enzymol. 1981;79(Pt B):51–59. doi: 10.1016/s0076-6879(81)79012-8. [DOI] [PubMed] [Google Scholar]
  15. Miyauchi J., Kelleher C. A., Yang Y. C., Wong G. G., Clark S. C., Minden M. D., Minkin S., McCulloch E. A. The effects of three recombinant growth factors, IL-3, GM-CSF, and G-CSF, on the blast cells of acute myeloblastic leukemia maintained in short-term suspension culture. Blood. 1987 Sep;70(3):657–663. [PubMed] [Google Scholar]
  16. Mizel S. B. Interleukin 1 and T cell activation. Immunol Rev. 1982;63:51–72. doi: 10.1111/j.1600-065x.1982.tb00411.x. [DOI] [PubMed] [Google Scholar]
  17. Mochizuki D. Y., Eisenman J. R., Conlon P. J., Larsen A. D., Tushinski R. J. Interleukin 1 regulates hematopoietic activity, a role previously ascribed to hemopoietin 1. Proc Natl Acad Sci U S A. 1987 Aug;84(15):5267–5271. doi: 10.1073/pnas.84.15.5267. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Moore M. A., Warren D. J. Synergy of interleukin 1 and granulocyte colony-stimulating factor: in vivo stimulation of stem-cell recovery and hematopoietic regeneration following 5-fluorouracil treatment of mice. Proc Natl Acad Sci U S A. 1987 Oct;84(20):7134–7138. doi: 10.1073/pnas.84.20.7134. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Nara N., McCulloch E. A. The proliferation in suspension of the progenitors of the blast cells in acute myeloblastic leukemia. Blood. 1985 Jun;65(6):1484–1493. [PubMed] [Google Scholar]
  20. Pettipher E. R., Higgs G. A., Henderson B. Interleukin 1 induces leukocyte infiltration and cartilage proteoglycan degradation in the synovial joint. Proc Natl Acad Sci U S A. 1986 Nov;83(22):8749–8753. doi: 10.1073/pnas.83.22.8749. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Pike B. L., Nossal G. J. Interleukin 1 can act as a B-cell growth and differentiation factor. Proc Natl Acad Sci U S A. 1985 Dec;82(23):8153–8157. doi: 10.1073/pnas.82.23.8153. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Rennick D., Yang G., Gemmell L., Lee F. Control of hemopoiesis by a bone marrow stromal cell clone: lipopolysaccharide- and interleukin-1-inducible production of colony-stimulating factors. Blood. 1987 Feb;69(2):682–691. [PubMed] [Google Scholar]
  23. Schmidt J. A., Mizel S. B., Cohen D., Green I. Interleukin 1, a potential regulator of fibroblast proliferation. J Immunol. 1982 May;128(5):2177–2182. [PubMed] [Google Scholar]
  24. Smith K. A., Lachman L. B., Oppenheim J. J., Favata M. F. The functional relationship of the interleukins. J Exp Med. 1980 Jun 1;151(6):1551–1556. doi: 10.1084/jem.151.6.1551. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Stanley E. R., Bartocci A., Patinkin D., Rosendaal M., Bradley T. R. Regulation of very primitive, multipotent, hemopoietic cells by hemopoietin-1. Cell. 1986 Jun 6;45(5):667–674. doi: 10.1016/0092-8674(86)90781-6. [DOI] [PubMed] [Google Scholar]
  26. Thomas P. S. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose. Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201–5205. doi: 10.1073/pnas.77.9.5201. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Vellenga E., Young D. C., Wagner K., Wiper D., Ostapovicz D., Griffin J. D. The effects of GM-CSF and G-CSF in promoting growth of clonogenic cells in acute myeloblastic leukemia. Blood. 1987 Jun;69(6):1771–1776. [PubMed] [Google Scholar]
  28. Welte K., Platzer E., Lu L., Gabrilove J. L., Levi E., Mertelsmann R., Moore M. A. Purification and biochemical characterization of human pluripotent hematopoietic colony-stimulating factor. Proc Natl Acad Sci U S A. 1985 Mar;82(5):1526–1530. doi: 10.1073/pnas.82.5.1526. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Wong G. G., Witek J. S., Temple P. A., Wilkens K. M., Leary A. C., Luxenberg D. P., Jones S. S., Brown E. L., Kay R. M., Orr E. C. Human GM-CSF: molecular cloning of the complementary DNA and purification of the natural and recombinant proteins. Science. 1985 May 17;228(4701):810–815. doi: 10.1126/science.3923623. [DOI] [PubMed] [Google Scholar]
  30. Young D. C., Griffin J. D. Autocrine secretion of GM-CSF in acute myeloblastic leukemia. Blood. 1986 Nov;68(5):1178–1181. [PubMed] [Google Scholar]
  31. Zucali J. R., Broxmeyer H. E., Dinarello C. A., Gross M. A., Weiner R. S. Regulation of early human hematopoietic (BFU-E and CFU-GEMM) progenitor cells in vitro by interleukin 1-induced fibroblast-conditioned medium. Blood. 1987 Jan;69(1):33–37. [PubMed] [Google Scholar]

Articles from The Journal of Experimental Medicine are provided here courtesy of The Rockefeller University Press

RESOURCES