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. 1985 Apr 1;161(4):669–686. doi: 10.1084/jem.161.4.669

Recognition of a leukemia-related antigen by an antiidiotypic antiserum to an anti-gp70 monoclonal antibody

PMCID: PMC2189059  PMID: 3856626

Abstract

The possibility that receptors for retroviral gp70 share structural elements with the antigen-binding sites of anti-retroviral gp70 antibodies was investigated. A monoclonal antibody (1416) was produced that reacted with the gp70 of a cloned recombinant leukemogenic retrovirus, termed P1. An antiidiotypic antiserum raised to 1416 was tested for its ability to bind to the thymic leukemia induced by P1 (P1 Thy). A membrane structure was identified on the surface of P1 Thy that reacted with the antibody against the idiotypic determinant of 1416. A similar structure was identified on the surface of several different, independently derived murine leukemias of T cell, B cell, and erythroid lineage. The expression of the idiotype-like determinant on these leukemia cells was independent of the serological relatedness of their expressed retroviral envelope glycoproteins to P1 gp70. The determinant recognized by the antiidiotype was not detected on normal lymphoid cells. The recognition by the anti-(anti-gp70) idiotype of determinants on unrelated murine leukemias suggests that receptors for different leukemogenic viruses may share common structures.

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Selected References

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  1. Baird S. M. Antigenic properties and molecular weights of murine leukemia virus-binding proteins. J Immunol. 1979 Apr;122(4):1389–1396. [PubMed] [Google Scholar]
  2. Bassin R. H., Ruscetti S., Ali I., Haapala D. K., Rein A. Normal DBA/2 mouse cells synthesize a glycoprotein which interferes with MCF virus infection. Virology. 1982 Nov;123(1):139–151. doi: 10.1016/0042-6822(82)90301-4. [DOI] [PubMed] [Google Scholar]
  3. Bassin R. H., Ruscetti S., Ali I., Haapala D. K., Rein A. Normal DBA/2 mouse cells synthesize a glycoprotein which interferes with MCF virus infection. Virology. 1982 Nov;123(1):139–151. doi: 10.1016/0042-6822(82)90301-4. [DOI] [PubMed] [Google Scholar]
  4. Bishayee S., Strand M., August J. T. Cellular membrane receptors for oncovirus envelope glycoprotein: properties of the binding reaction and influence of different reagents on the substrate and the receptors. Arch Biochem Biophys. 1978 Jul;189(1):161–171. doi: 10.1016/0003-9861(78)90129-7. [DOI] [PubMed] [Google Scholar]
  5. Bona C. A. Parallel sets and the internal image of antigen within the idiotypic network. Fed Proc. 1984 Jul;43(10):2558–2562. [PubMed] [Google Scholar]
  6. Choppin J., Schaffar-Deshayes L., Debré P., Lévy J. P. Lymphoid cell surface receptor for Moloney leukemia virus envelope glycoprotein gp71. I. Binding characteristics. J Immunol. 1981 Jun;126(6):2347–2351. [PubMed] [Google Scholar]
  7. DeLarco J., Todaro G. J. Membrane receptors for murine leukemia viruses: characterization using the purified viral envelope glycoprotein, gp71. Cell. 1976 Jul;8(3):365–371. doi: 10.1016/0092-8674(76)90148-3. [DOI] [PubMed] [Google Scholar]
  8. Elder J. H., Gautsch J. W., Jensen F. C., Lerner R. A., Hartley J. W., Rowe W. P. Biochemical evidence that MCF murine leukemia viruses are envelope (env) gene recombinants. Proc Natl Acad Sci U S A. 1977 Oct;74(10):4676–4680. doi: 10.1073/pnas.74.10.4676. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Enghofer E., Glaudemans C. P., Bosma M. J. Immunoglobulins with different specificities have similar idiotypes. Mol Immunol. 1979 Dec;16(12):1103–1110. doi: 10.1016/0161-5890(79)90044-0. [DOI] [PubMed] [Google Scholar]
  10. Fowler A. K., Twardzik D. R., Reed C. D., Weislow O. S., Hellman A. Binding characteristics of Rauscher leukemia virus envelope glycoprotein gp71 to murine lymphoid cells. J Virol. 1977 Dec;24(3):729–735. doi: 10.1128/jvi.24.3.729-735.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Green N., Hiai H., Elder J. H., Schwartz R. S., Khiroya R. H., Thomas C. Y., Tsichlis P. N., Coffin J. M. Expression of leukemogenic recombinant viruses associated with a recessive gene in HRS/J mice. J Exp Med. 1980 Aug 1;152(2):249–264. doi: 10.1084/jem.152.2.249. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Hartley J. W., Rowe W. P. Naturally occurring murine leukemia viruses in wild mice: characterization of a new "amphotropic" class. J Virol. 1976 Jul;19(1):19–25. doi: 10.1128/jvi.19.1.19-25.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Hartley J. W., Wolford N. K., Old L. J., Rowe W. P. A new class of murine leukemia virus associated with development of spontaneous lymphomas. Proc Natl Acad Sci U S A. 1977 Feb;74(2):789–792. doi: 10.1073/pnas.74.2.789. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Hiai H., Morrissey P., Khiroya R., Schwartz R. S. Selective expression of xenotropic virus in congenic HRS/J (hairless) mice. Nature. 1977 Nov 17;270(5634):247–249. doi: 10.1038/270247a0. [DOI] [PubMed] [Google Scholar]
  15. Kauffman R. S., Noseworthy J. H., Nepom J. T., Finberg R., Fields B. N., Greene M. I. Cell receptors for the mammalian reovirus. II. Monoclonal anti-idiotypic antibody blocks viral binding to cells. J Immunol. 1983 Nov;131(5):2539–2541. [PubMed] [Google Scholar]
  16. Kawashima K., Ikeda H., Hartley J. W., Stockert E., Rowe W. P., Old L. J. Changes in expression of murine leukemia virus antigens and production of xenotropic virus in the late preleukemic period in AKR mice. Proc Natl Acad Sci U S A. 1976 Dec;73(12):4680–4684. doi: 10.1073/pnas.73.12.4680. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Kranz D. M., Sherman D. H., Sitkovsky M. V., Pasternack M. S., Eisen H. N. Immunoprecipitation of cell surface structures of cloned cytotoxic T lymphocytes by clone-specific antisera. Proc Natl Acad Sci U S A. 1984 Jan;81(2):573–577. doi: 10.1073/pnas.81.2.573. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Köhler G., Milstein C. Continuous cultures of fused cells secreting antibody of predefined specificity. Nature. 1975 Aug 7;256(5517):495–497. doi: 10.1038/256495a0. [DOI] [PubMed] [Google Scholar]
  19. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  20. Landen B., Fox C. F. Isolation of BPgp70, a fibroblast receptor for the envelope antigen of Rauscher murine leukemia virus. Proc Natl Acad Sci U S A. 1980 Aug;77(8):4988–4992. doi: 10.1073/pnas.77.8.4988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Lee J. C., Ihle J. N. Chronic immune stimulation is required for Moloney leukaemia virus-induced lymphomas. Nature. 1981 Jan 29;289(5796):407–409. doi: 10.1038/289407a0. [DOI] [PubMed] [Google Scholar]
  22. Lee J. C., Ihle J. N. Increased responses to lymphokines are correlated with preleukemia in mice inoculated with Moloney leukemia virus. Proc Natl Acad Sci U S A. 1981 Dec;78(12):7712–7716. doi: 10.1073/pnas.78.12.7712. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Markwell M. A. A new solid-state reagent to iodinate proteins. I. Conditions for the efficient labeling of antiserum. Anal Biochem. 1982 Sep 15;125(2):427–432. doi: 10.1016/0003-2697(82)90025-2. [DOI] [PubMed] [Google Scholar]
  24. McGrath M. S., Decléve A., Lieberman M., Kaplan H. S., Weissman I. L. Specificity of cell surface virus receptors on radiation leukemia virus and radiation-induced thymic lymphomas. J Virol. 1978 Dec;28(3):819–827. doi: 10.1128/jvi.28.3.819-827.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. McGrath M. S., Pillemer E., Weissman I. L. Murine leukaemogenesis: monoclonal antibodies to T-cell determinants arrest T-lymphoma cell proliferation. Nature. 1980 May 22;285(5762):259–261. doi: 10.1038/285259a0. [DOI] [PubMed] [Google Scholar]
  26. McGrath M. S., Weissman I. L. AKR leukemogenesis: identification and biological significance of thymic lymphoma receptors for AKR retroviruses. Cell. 1979 May;17(1):65–75. doi: 10.1016/0092-8674(79)90295-2. [DOI] [PubMed] [Google Scholar]
  27. McGrath M., Witte O., Pincus T., Weissman I. L. Retrovirus purification: method that conserves envelope glycoprotein and maximizes infectivity. J Virol. 1978 Mar;25(3):923–927. doi: 10.1128/jvi.25.3.923-927.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Moennig V., Frank H., Hunsmann G., Schneider I., Schafer W. Properties of mouse leukemia viruses. VII. The major viral glycoprotein of friend leukemia virus. Isolation and physicochemical properties. Virology. 1974 Sep;61(1):100–111. doi: 10.1016/0042-6822(74)90245-1. [DOI] [PubMed] [Google Scholar]
  29. Nepom J. T., Weiner H. L., Dichter M. A., Tardieu M., Spriggs D. R., Gramm C. F., Powers M. L., Fields B. N., Greene M. I. Identification of a hemagglutinin-specific idiotype associated with reovirus recognition shared by lymphoid and neural cells. J Exp Med. 1982 Jan 1;155(1):155–167. doi: 10.1084/jem.155.1.155. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Noseworthy J. H., Fields B. N., Dichter M. A., Sobotka C., Pizer E., Perry L. L., Nepom J. T., Greene M. I. Cell receptors for the mammalian reovirus. I. Syngeneic monoclonal anti-idiotypic antibody identifies a cell surface receptor for reovirus. J Immunol. 1983 Nov;131(5):2533–2538. [PubMed] [Google Scholar]
  31. Oudin J., Cazenave P. A. Similar idiotypic specificities in immunoglobulin fractions with different antibody functions or even without detectable antibody function. Proc Natl Acad Sci U S A. 1971 Oct;68(10):2616–2620. doi: 10.1073/pnas.68.10.2616. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Pinter A., Lieman-Hurwitz J., Fleissner E. The nature of the association between the murine leukemia virus envelope proteins. Virology. 1978 Dec;91(2):345–351. doi: 10.1016/0042-6822(78)90382-3. [DOI] [PubMed] [Google Scholar]
  33. Robinson P. J., Hunsmann G., Schneider J., Schirrmacher V. Possible cell surface receptor for Friend murine leukemia virus isolated with viral envelope glycoprotein complexes. J Virol. 1980 Oct;36(1):291–294. doi: 10.1128/jvi.36.1.291-294.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Rommelaere J., Faller D. V., Hopkins N. Characterization and mapping of RNase T1-resistant oligonucleotides derived from the genomes of Akv and MCF murine leukemia viruses. Proc Natl Acad Sci U S A. 1978 Jan;75(1):495–499. doi: 10.1073/pnas.75.1.495. [DOI] [PMC free article] [PubMed] [Google Scholar]
  35. Schaffar-Deshayes L., Choppin J., Lévy J. P. Lymphoid cell surface receptor for Moloney leukemia virus envelope glycoprotein gp71. II. Isolation of the receptor. J Immunol. 1981 Jun;126(6):2352–2354. [PubMed] [Google Scholar]
  36. Schreiber A. B., Couraud P. O., Andre C., Vray B., Strosberg A. D. Anti-alprenolol anti-idiotypic antibodies bind to beta-adrenergic receptors and modulate catecholamine-sensitive adenylate cyclase. Proc Natl Acad Sci U S A. 1980 Dec;77(12):7385–7389. doi: 10.1073/pnas.77.12.7385. [DOI] [PMC free article] [PubMed] [Google Scholar]
  37. Sege K., Peterson P. A. Use of anti-idiotypic antibodies as cell-surface receptor probes. Proc Natl Acad Sci U S A. 1978 May;75(5):2443–2447. doi: 10.1073/pnas.75.5.2443. [DOI] [PMC free article] [PubMed] [Google Scholar]
  38. Thiry L., Cogniaux-LeClerc J., Olislager R., Sprecher-Goldberger S., Buekens P. Cell receptors for baboon endogenous virus recognized by monoclonal antibodies. J Virol. 1983 Dec;48(3):697–708. doi: 10.1128/jvi.48.3.697-708.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  39. Thomas C. Y., Khiroya R., Schwartz R. S., Coffin J. M. Role of recombinant ecotropic and polytropic viruses in the development of spontaneous thymic lymphomas in HRS/J mice. J Virol. 1984 May;50(2):397–407. doi: 10.1128/jvi.50.2.397-407.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  40. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. doi: 10.1073/pnas.76.9.4350. [DOI] [PMC free article] [PubMed] [Google Scholar]
  41. Twardzik D. R., Fowler A. K., Weislow O. S., Hegamyer G. A., Hellman A. Cell surface binding proteins for the major envelope glycoprotein of murine leukemia virus. Proc Soc Exp Biol Med. 1979 Nov;162(2):304–309. doi: 10.3181/00379727-162-40670. [DOI] [PubMed] [Google Scholar]
  42. Wassermann N. H., Penn A. S., Freimuth P. I., Treptow N., Wentzel S., Cleveland W. L., Erlanger B. F. Anti-idiotypic route to anti-acetylcholine receptor antibodies and experimental myasthenia gravis. Proc Natl Acad Sci U S A. 1982 Aug;79(15):4810–4814. doi: 10.1073/pnas.79.15.4810. [DOI] [PMC free article] [PubMed] [Google Scholar]
  43. Zielinski C. C., Waksal S. D., Tempelis L. D., Khiroya R. H., Schwartz R. S. Surface phenotypes in T-cell leukaemia are determined by oncogenic retroviruses. Nature. 1980 Dec 4;288(5790):489–491. doi: 10.1038/288489a0. [DOI] [PubMed] [Google Scholar]

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