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. 1988 Oct 1;168(4):1339–1349. doi: 10.1084/jem.168.4.1339

Interaction and sequence diversity among T15 VH genes in CBA/J mice

PMCID: PMC2189069  PMID: 3139820

Abstract

Nucleotide sequences of the four genes composing the T15 heavy chain variable region (VH) family of the CBA/J mouse have been determined. Comparison of these sequences with their published BALB/c and C57BL/10 homologues reveals that nucleotide differences found between given alleles of two strains, i.e., CBA/J and BALB/c, are observed in other family members of the same strain. We suggest that these patterns of sequence variation are most readily explained by gene interaction (conversion). Additionally, the sequence of a CBA/J hybridoma, 6G6, proposed to have been generated by gene conversion, is directly encoded by the CBA/J V11 gene indicating that the putative conversion has occurred meiotically in the germline. These results are consistent with the premise that gene correction is occurring frequently among members of this family and that such processes may contribute significantly to the evolution of Ig variable region genes even in the relatively short time frame of inbred strain derivation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Akimenko M. A., Mariamé B., Rougeon F. Evolution of the immunoglobulin kappa light chain locus in the rabbit: evidence for differential gene conversion events. Proc Natl Acad Sci U S A. 1986 Jul;83(14):5180–5183. doi: 10.1073/pnas.83.14.5180. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bentley D. L., Rabbitts T. H. Evolution of immunoglobulin V genes: evidence indicating that recently duplicated human V kappa sequences have diverged by gene conversion. Cell. 1983 Jan;32(1):181–189. doi: 10.1016/0092-8674(83)90508-1. [DOI] [PubMed] [Google Scholar]
  3. Benton W. D., Davis R. W. Screening lambdagt recombinant clones by hybridization to single plaques in situ. Science. 1977 Apr 8;196(4286):180–182. doi: 10.1126/science.322279. [DOI] [PubMed] [Google Scholar]
  4. Claflin J. L., Rudikoff S. Structural evidence for a polymorphic or allelic form of the heavy chain variable region. J Immunol. 1979 Apr;122(4):1402–1406. [PubMed] [Google Scholar]
  5. Claflin J. L., Rudikoff S. Uniformity in a clonal repertoire: a case for a germ-line basis of antibody diversity. Cold Spring Harb Symp Quant Biol. 1977;41(Pt 2):725–734. doi: 10.1101/sqb.1977.041.01.083. [DOI] [PubMed] [Google Scholar]
  6. Clarke S. H., Claflin J. L., Potter M., Rudikoff S. Polymorphism in anti-phosphocholine antibodies reflecting evolution of immunoglobulin families. J Exp Med. 1983 Jan 1;157(1):98–113. doi: 10.1084/jem.157.1.98. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Clarke S. H., Claflin J. L., Rudikoff S. Polymorphism in immunoglobulin heavy chains suggesting gene conversion. Proc Natl Acad Sci U S A. 1982 May;79(10):3280–3284. doi: 10.1073/pnas.79.10.3280. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Clarke S. H., Rudikoff S. Evidence for gene conversion among immunoglobulin heavy chain variable region genes. J Exp Med. 1984 Mar 1;159(3):773–782. doi: 10.1084/jem.159.3.773. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Crews S., Griffin J., Huang H., Calame K., Hood L. A single VH gene segment encodes the immune response to phosphorylcholine: somatic mutation is correlated with the class of the antibody. Cell. 1981 Jul;25(1):59–66. doi: 10.1016/0092-8674(81)90231-2. [DOI] [PubMed] [Google Scholar]
  10. Feinberg A. P., Vogelstein B. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem. 1983 Jul 1;132(1):6–13. doi: 10.1016/0003-2697(83)90418-9. [DOI] [PubMed] [Google Scholar]
  11. Fogel S., Mortimer R., Lusnak K., Tavares F. Meiotic gene conversion: a signal of the basic recombination event in yeast. Cold Spring Harb Symp Quant Biol. 1979;43(Pt 2):1325–1341. doi: 10.1101/sqb.1979.043.01.152. [DOI] [PubMed] [Google Scholar]
  12. Hamada H., Seidman M., Howard B. H., Gorman C. M. Enhanced gene expression by the poly(dT-dG).poly(dC-dA) sequence. Mol Cell Biol. 1984 Dec;4(12):2622–2630. doi: 10.1128/mcb.4.12.2622. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Huang H., Crews S., Hood L. An immunoglobulin VH pseudogene. J Mol Appl Genet. 1981;1(2):93–101. [PubMed] [Google Scholar]
  14. Jackson J. A., Fink G. R. Gene conversion between duplicated genetic elements in yeast. Nature. 1981 Jul 23;292(5821):306–311. doi: 10.1038/292306a0. [DOI] [PubMed] [Google Scholar]
  15. Jouvin-Marche E., Heller M., Rudikoff S. Gene correction in the evolution of the T cell receptor beta chain. J Exp Med. 1986 Dec 1;164(6):2083–2088. doi: 10.1084/jem.164.6.2083. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Klein H. L., Petes T. D. Intrachromosomal gene conversion in yeast. Nature. 1981 Jan 15;289(5794):144–148. doi: 10.1038/289144a0. [DOI] [PubMed] [Google Scholar]
  17. Krawinkel U., Zoebelein G., Brüggemann M., Radbruch A., Rajewsky K. Recombination between antibody heavy chain variable-region genes: evidence for gene conversion. Proc Natl Acad Sci U S A. 1983 Aug;80(16):4997–5001. doi: 10.1073/pnas.80.16.4997. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Morris J., Kushner S. R., Ivarie R. The simple repeat poly(dT-dG).poly(dC-dA) common to eukaryotes is absent from eubacteria and archaebacteria and rare in protozoans. Mol Biol Evol. 1986 Jul;3(4):343–355. doi: 10.1093/oxfordjournals.molbev.a040399. [DOI] [PubMed] [Google Scholar]
  19. Nagylaki T., Petes T. D. Intrachromosomal gene conversion and the maintenance of sequence homogeneity among repeated genes. Genetics. 1982 Feb;100(2):315–337. doi: 10.1093/genetics/100.2.315. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Nordheim A., Rich A. The sequence (dC-dA)n X (dG-dT)n forms left-handed Z-DNA in negatively supercoiled plasmids. Proc Natl Acad Sci U S A. 1983 Apr;80(7):1821–1825. doi: 10.1073/pnas.80.7.1821. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Ollo R., Rougeon F. Gene conversion and polymorphism: generation of mouse immunoglobulin gamma 2a chain alleles by differential gene conversion by gamma 2b chain gene. Cell. 1983 Feb;32(2):515–523. doi: 10.1016/0092-8674(83)90471-3. [DOI] [PubMed] [Google Scholar]
  22. Perlmutter R. M., Berson B., Griffin J. A., Hood L. Diversity in the germline antibody repertoire. Molecular evolution of the T15 VN gene family. J Exp Med. 1985 Dec 1;162(6):1998–2016. doi: 10.1084/jem.162.6.1998. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Perlmutter R. M., Crews S. T., Douglas R., Sorensen G., Johnson N., Nivera N., Gearhart P. J., Hood L. The generation of diversity in phosphorylcholine-binding antibodies. Adv Immunol. 1984;35:1–37. doi: 10.1016/s0065-2776(08)60572-6. [DOI] [PubMed] [Google Scholar]
  24. Reynaud C. A., Anquez V., Grimal H., Weill J. C. A hyperconversion mechanism generates the chicken light chain preimmune repertoire. Cell. 1987 Feb 13;48(3):379–388. doi: 10.1016/0092-8674(87)90189-9. [DOI] [PubMed] [Google Scholar]
  25. Sanger F., Coulson A. R., Barrell B. G., Smith A. J., Roe B. A. Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing. J Mol Biol. 1980 Oct 25;143(2):161–178. doi: 10.1016/0022-2836(80)90196-5. [DOI] [PubMed] [Google Scholar]
  26. Slightom J. L., Blechl A. E., Smithies O. Human fetal G gamma- and A gamma-globin genes: complete nucleotide sequences suggest that DNA can be exchanged between these duplicated genes. Cell. 1980 Oct;21(3):627–638. doi: 10.1016/0092-8674(80)90426-2. [DOI] [PubMed] [Google Scholar]
  27. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  28. Streisinger G., Okada Y., Emrich J., Newton J., Tsugita A., Terzaghi E., Inouye M. Frameshift mutations and the genetic code. This paper is dedicated to Professor Theodosius Dobzhansky on the occasion of his 66th birthday. Cold Spring Harb Symp Quant Biol. 1966;31:77–84. doi: 10.1101/sqb.1966.031.01.014. [DOI] [PubMed] [Google Scholar]
  29. Thompson C. B., Neiman P. E. Somatic diversification of the chicken immunoglobulin light chain gene is limited to the rearranged variable gene segment. Cell. 1987 Feb 13;48(3):369–378. doi: 10.1016/0092-8674(87)90188-7. [DOI] [PubMed] [Google Scholar]

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