Abstract
The CD3+, IL-2-dependent normal human thymocyte clone, CII, expresses on its surface a CD3-associated gamma/delta TCR. We have further elucidated the structure of this receptor from the nucleotide sequence of cDNA and genomic clones from CII that encode functional TCR-gamma and -delta chains. We find that the CII line expresses a C gamma 2 constant region that is a polymorphic form lacking a copy of an internal exon; the sequence of this constant region accounts for the size of the gamma chain and noncovalent linkage of gamma and delta chains in the CII TCR. The V gamma region used for the CII TCR is identical to the several previously characterized expressed human V gamma segments. Possible implications of this finding are discussed.
Full Text
The Full Text of this article is available as a PDF (370.9 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bank I., DePinho R. A., Brenner M. B., Cassimeris J., Alt F. W., Chess L. A functional T3 molecule associated with a novel heterodimer on the surface of immature human thymocytes. Nature. 1986 Jul 10;322(6075):179–181. doi: 10.1038/322179a0. [DOI] [PubMed] [Google Scholar]
- Borst J., van de Griend R. J., van Oostveen J. W., Ang S. L., Melief C. J., Seidman J. G., Bolhuis R. L. A T-cell receptor gamma/CD3 complex found on cloned functional lymphocytes. Nature. 1987 Feb 19;325(6106):683–688. doi: 10.1038/325683a0. [DOI] [PubMed] [Google Scholar]
- Brenner M. B., McLean J., Scheft H., Riberdy J., Ang S. L., Seidman J. G., Devlin P., Krangel M. S. Two forms of the T-cell receptor gamma protein found on peripheral blood cytotoxic T lymphocytes. Nature. 1987 Feb 19;325(6106):689–694. doi: 10.1038/325689a0. [DOI] [PubMed] [Google Scholar]
- DePinho R. A., Hatton K. S., Tesfaye A., Yancopoulos G. D., Alt F. W. The human myc gene family: structure and activity of L-myc and an L-myc pseudogene. Genes Dev. 1987 Dec;1(10):1311–1326. doi: 10.1101/gad.1.10.1311. [DOI] [PubMed] [Google Scholar]
- Elliott J. F., Rock E. P., Patten P. A., Davis M. M., Chien Y. H. The adult T-cell receptor delta-chain is diverse and distinct from that of fetal thymocytes. Nature. 1988 Feb 18;331(6157):627–631. doi: 10.1038/331627a0. [DOI] [PubMed] [Google Scholar]
- Hata S., Brenner M. B., Krangel M. S. Identification of putative human T cell receptor delta complementary DNA clones. Science. 1987 Oct 30;238(4827):678–682. doi: 10.1126/science.3499667. [DOI] [PubMed] [Google Scholar]
- Hochstenbach F., Parker C., McLean J., Gieselmann V., Band H., Bank I., Chess L., Spits H., Strominger J. L., Seidman J. G. Characterization of a third form of the human T cell receptor gamma/delta. J Exp Med. 1988 Aug 1;168(2):761–776. doi: 10.1084/jem.168.2.761. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Huck S., Dariavach P., Lefranc M. P. Variable region genes in the human T-cell rearranging gamma (TRG) locus: V-J junction and homology with the mouse genes. EMBO J. 1988 Mar;7(3):719–726. doi: 10.1002/j.1460-2075.1988.tb02868.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Littman D. R., Newton M., Crommie D., Ang S. L., Seidman J. G., Gettner S. N., Weiss A. Characterization of an expressed CD3-associated Ti gamma-chain reveals C gamma domain polymorphism. Nature. 1987 Mar 5;326(6108):85–88. doi: 10.1038/326085a0. [DOI] [PubMed] [Google Scholar]
- Loh E. Y., Lanier L. L., Turck C. W., Littman D. R., Davis M. M., Chien Y. H., Weiss A. Identification and sequence of a fourth human T cell antigen receptor chain. Nature. 1987 Dec 10;330(6148):569–572. doi: 10.1038/330569a0. [DOI] [PubMed] [Google Scholar]
- Marrack P., Kappler J. The T cell receptor. Science. 1987 Nov 20;238(4830):1073–1079. doi: 10.1126/science.3317824. [DOI] [PubMed] [Google Scholar]
- Pelicci P. G., Subar M., Weiss A., Dalla-Favera R., Littman D. R. Molecular diversity of the human T-gamma constant region genes. Science. 1987 Aug 28;237(4818):1051–1055. doi: 10.1126/science.3112943. [DOI] [PubMed] [Google Scholar]
- Takihara Y., Champagne E., Griesser H., Kimura N., Tkachuk D., Reimann J., Okada A., Alt F. W., Chess L., Minden M. Sequence and organization of the human T cell delta chain gene. Eur J Immunol. 1988 Feb;18(2):283–287. doi: 10.1002/eji.1830180216. [DOI] [PubMed] [Google Scholar]