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. 1988 Dec 1;168(6):2023–2030. doi: 10.1084/jem.168.6.2023

Strain variation in the frequency of Abelson murine leukemia virus- transformed fetal liver pre-B cells bearing complete immunoglobulin heavy chain rearrangements

PMCID: PMC2189137  PMID: 3264321

Abstract

Fetal liver Abelson pre-B cell lines obtained from CBA/Tufts.xid and (CBA/Tufts.xid x CBA/Tufts)F1 mice have complete VDJH rearrangements on at least one allele. Such high frequencies of VDJH rearrangements have previously been observed in adult derived but not fetal liver derived Abelson pre-B cell lines. Genetic analyses suggest that CBA/Tufts.xid carries an autosomal dominant gene(s) that determines the predominance of VDJH rearrangements among transformants. This autosomal gene(s) might affect the intrinsic development of the early B cell lineage in the fetus or the fetal microenvironment, expanding pre-B cells of the "more mature" VDJH phenotype.

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Selected References

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  1. Alt F. W., Yancopoulos G. D., Blackwell T. K., Wood C., Thomas E., Boss M., Coffman R., Rosenberg N., Tonegawa S., Baltimore D. Ordered rearrangement of immunoglobulin heavy chain variable region segments. EMBO J. 1984 Jun;3(6):1209–1219. doi: 10.1002/j.1460-2075.1984.tb01955.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Alt F., Rosenberg N., Lewis S., Thomas E., Baltimore D. Organization and reorganization of immunoglobulin genes in A-MULV-transformed cells: rearrangement of heavy but not light chain genes. Cell. 1981 Dec;27(2 Pt 1):381–390. doi: 10.1016/0092-8674(81)90421-9. [DOI] [PubMed] [Google Scholar]
  3. Brodeur P. H., Riblet R. The immunoglobulin heavy chain variable region (Igh-V) locus in the mouse. I. One hundred Igh-V genes comprise seven families of homologous genes. Eur J Immunol. 1984 Oct;14(10):922–930. doi: 10.1002/eji.1830141012. [DOI] [PubMed] [Google Scholar]
  4. Denis K. A., Treiman L. J., St Claire J. I., Witte O. N. Long-term cultures of murine fetal liver retain very early B lymphoid phenotype. J Exp Med. 1984 Oct 1;160(4):1087–1101. doi: 10.1084/jem.160.4.1087. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Forrester L. M., Ansell J. D., Micklem H. S. Development of B lymphocytes in mice heterozygous for the X-linked immunodeficiency (xid) mutation. xid inhibits development of all splenic and lymph node B cells at a stage subsequent to their initial formation in bone marrow. J Exp Med. 1987 Apr 1;165(4):949–958. doi: 10.1084/jem.165.4.949. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Goff S. P., Gilboa E., Witte O. N., Baltimore D. Structure of the Abelson murine leukemia virus genome and the homologous cellular gene: studies with cloned viral DNA. Cell. 1980 Dec;22(3):777–785. doi: 10.1016/0092-8674(80)90554-1. [DOI] [PubMed] [Google Scholar]
  7. Karagogeos D., Rosenberg N., Wortis H. H. Early arrest of B cell development in nude, X-linked immune-deficient mice. Eur J Immunol. 1986 Sep;16(9):1125–1130. doi: 10.1002/eji.1830160916. [DOI] [PubMed] [Google Scholar]
  8. Lamar E. E., Palmer E. Y-encoded, species-specific DNA in mice: evidence that the Y chromosome exists in two polymorphic forms in inbred strains. Cell. 1984 May;37(1):171–177. doi: 10.1016/0092-8674(84)90312-x. [DOI] [PubMed] [Google Scholar]
  9. Marcu K. B., Banerji J., Penncavage N. A., Lang R., Arnheim N. 5' flanking region of immunoglobulin heavy chain constant region genes displays length heterogeneity in germlines of inbred mouse strains. Cell. 1980 Nov;22(1 Pt 1):187–196. doi: 10.1016/0092-8674(80)90167-1. [DOI] [PubMed] [Google Scholar]
  10. Perlmutter R. M., Kearney J. F., Chang S. P., Hood L. E. Developmentally controlled expression of immunoglobulin VH genes. Science. 1985 Mar 29;227(4694):1597–1601. doi: 10.1126/science.3975629. [DOI] [PubMed] [Google Scholar]
  11. Reth M. G., Jackson S., Alt F. W. VHDJH formation and DJH replacement during pre-B differentiation: non-random usage of gene segments. EMBO J. 1986 Sep;5(9):2131–2138. doi: 10.1002/j.1460-2075.1986.tb04476.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Rosenberg N., Baltimore D. A quantitative assay for transformation of bone marrow cells by Abelson murine leukemia virus. J Exp Med. 1976 Jun 1;143(6):1453–1463. doi: 10.1084/jem.143.6.1453. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Sprent J., Bruce J. Physiology of B cells in mice with X-linked immunodeficiency (xid). III. Disappearance of xid B cells in double bone marrow chimeras. J Exp Med. 1984 Sep 1;160(3):711–723. doi: 10.1084/jem.160.3.711. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Sugiyama H., Maeda T., Tani Y., Miyake S., Oka Y., Komori T., Ogawa H., Soma T., Minami Y., Sakato N. Selective use of the VHQ52 family in functional VH to DJH rearrangements in a B precursor cell line. J Exp Med. 1987 Aug 1;166(2):607–612. doi: 10.1084/jem.166.2.607. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Tonegawa S. Somatic generation of antibody diversity. Nature. 1983 Apr 14;302(5909):575–581. doi: 10.1038/302575a0. [DOI] [PubMed] [Google Scholar]
  16. Wortis H. H., Burkly L., Hughes D., Roschelle S., Waneck G. Lack of mature B cells in nude mice with X-linked immune deficiency. J Exp Med. 1982 Mar 1;155(3):903–913. doi: 10.1084/jem.155.3.903. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Yancopoulos G. D., Desiderio S. V., Paskind M., Kearney J. F., Baltimore D., Alt F. W. Preferential utilization of the most JH-proximal VH gene segments in pre-B-cell lines. Nature. 1984 Oct 25;311(5988):727–733. doi: 10.1038/311727a0. [DOI] [PubMed] [Google Scholar]
  18. Yoshida N., Radbruch A., Rajewsky K. Ig gene rearrangement and expression in the progeny of B-cell progenitors in the course of clonal expansion in bone marrow cultures. EMBO J. 1987 Sep;6(9):2735–2741. doi: 10.1002/j.1460-2075.1987.tb02567.x. [DOI] [PMC free article] [PubMed] [Google Scholar]

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