Abstract
Rat thymic grafts reconstituted T cell functions of BALB/c nude (nu/nu) mice to a considerable degree, but multiple organ-localized autoimmune diseases such as oophoritis and thyroiditis generally developed. The effector cell population in this autoimmune model was studied by adoptive transfer of the lesions into syngeneic nude mice. The transfer activity was not diminished when spleen cells were incubated with antiserum against rat cell antigen and C, but the activity was completely vanished by incubation with anti-Thy-1.2 plus C, indicating that the effector cells are T cells of mouse origin. Elimination of the L3T4+ subset virtually abolished the transfer activity, whereas that of the Lyt-2+ subset did not, indicating that the effector cells are L3T4+. Positive selection experiments by FACS also demonstrated that L3T4+ cells, but not Lyt-2+ cells, were capable of inducing the lesion, confirming the results with depletion experiments described above.
Full Text
The Full Text of this article is available as a PDF (484.3 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bendelac A., Carnaud C., Boitard C., Bach J. F. Syngeneic transfer of autoimmune diabetes from diabetic NOD mice to healthy neonates. Requirement for both L3T4+ and Lyt-2+ T cells. J Exp Med. 1987 Oct 1;166(4):823–832. doi: 10.1084/jem.166.4.823. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Golding H., Munitz T. I., Singer A. Characterization of antigen-specific, Ia-restricted, L3T4+ cytolytic T lymphocytes and assessment of thymic influence on their self specificity. J Exp Med. 1985 Sep 1;162(3):943–961. doi: 10.1084/jem.162.3.943. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jotereau F., Heuze F., Salomon-Vie V., Gascan H. Cell kinetics in the fetal mouse thymus: precursor cell input, proliferation, and emigration. J Immunol. 1987 Feb 15;138(4):1026–1030. [PubMed] [Google Scholar]
- Lemire J. M., Weigle W. O. Passive transfer of experimental allergic encephalomyelitis by myelin basic protein-specific L3T4+ T cell clones possessing several functions. J Immunol. 1986 Nov 15;137(10):3169–3174. [PubMed] [Google Scholar]
- Owen J. J., Ritter M. A. Tissue interaction in the development of thymus lymphocytes. J Exp Med. 1969 Feb 1;129(2):431–442. doi: 10.1084/jem.129.2.431. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Romball C. G., Weigle W. O. Transfer of experimental autoimmune thyroiditis with T cell clones. J Immunol. 1987 Feb 15;138(4):1092–1098. [PubMed] [Google Scholar]
- Sakaguchi S., Takahashi T., Nishizuka Y. Study on cellular events in postthymectomy autoimmune oophoritis in mice. I. Requirement of Lyt-1 effector cells for oocytes damage after adoptive transfer. J Exp Med. 1982 Dec 1;156(6):1565–1576. doi: 10.1084/jem.156.6.1565. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Taguchi O., Nishizuka Y. Autoimmune oophoritis in thymectomized mice: T cell requirement in adoptive cell transfer. Clin Exp Immunol. 1980 Nov;42(2):324–331. [PMC free article] [PubMed] [Google Scholar]
- Taguchi O., Nishizuka Y. Self tolerance and localized autoimmunity. Mouse models of autoimmune disease that suggest tissue-specific suppressor T cells are involved in self tolerance. J Exp Med. 1987 Jan 1;165(1):146–156. doi: 10.1084/jem.165.1.146. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Taguchi O., Takahashi T., Seto M., Namikawa R., Matsuyama M., Nishizuka Y. Development of multiple organ-localized autoimmune diseases in nude mice after reconstitution of T cell function by rat fetal thymus graft. J Exp Med. 1986 Jul 1;164(1):60–71. doi: 10.1084/jem.164.1.60. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Trotter J., Sriram S., Rassenti L., Chou C. H., Fritz R. B., Steinman L. Characterization of T cell lines and clones from SJL/J and (BALB/c x SJL/J)F1 mice specific for myelin basic protein. J Immunol. 1985 Apr;134(4):2322–2327. [PubMed] [Google Scholar]