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. 1989 Jan 1;169(1):315–320. doi: 10.1084/jem.169.1.315

Clonal expansion of human T lymphocytes initiated by dendritic cells

PMCID: PMC2189191  PMID: 2562849

Abstract

The accessory cell requirements for cloning T cells in the presence of lectin and T cell growth factors were examined with cells from human peripheral blood. We found that dendritic cells were active and perhaps essential. Single CD4+ lymphocytes could be cloned with 80% efficiency, and CD8+ cells with 50-60% efficiency if 10(3) syngeneic or allogeneic dendritic cells were added. Some T cell clones developed even with one dendritic cell. Monocytes or B lymphocytes from blood were at least 100- fold weaker in supporting clonal growth. These findings suggest a specialized feeder cell requirement, namely dendritic cells, for cloning T lymphocytes from single resting precursors.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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