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. 1989 Jan 1;169(1):297–302. doi: 10.1084/jem.169.1.297

Recognition of oligonucleotide-encoded T cell epitopes introduced into a gene unrelated to the original antigen

PMCID: PMC2189194  PMID: 2462610

Abstract

We have previously demonstrated that H-2Kd-restricted CTL specific for HLA-CW3 or HLA-A24 can recognize synthetic peptides corresponding to residues 170-182 of the HLA molecules. Synthetic oligonucleotides encoding region 170-182 of CW3 or A24 were inserted into the influenza nucleoprotein (NP) gene. We demonstrate herein that P815 (H-2d) cells transfected with the NP-oligo recombinant genes are specifically lysed by HLA-specific Kd-restricted CTL clones. Our results imply that there must be a high degree of flexibility for the expression of T cell epitopes in different molecular contexts.

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Selected References

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