Skip to main content
PMC home page
The Journal of Experimental Medicine logoLink to The Journal of Experimental Medicine
. 1989 May 1;169(5):1533–1541. doi: 10.1084/jem.169.5.1533

Analysis of V beta 17a expression in new mouse strains bearing the V beta a haplotype

PMCID: PMC2189295  PMID: 2523951

Abstract

A set of new mouse strains were produced that carry the V beta a haplotype of the TCR-alpha/beta and any of a number of different H-2 haplotypes on backgrounds derived from related C57BL, C57L, and C57BR mice. Study of V beta 17a expression in these mice confirms the association between the presence of IE and the deletion of V beta 17a+ T cells. A second H-2 gene causing deletion of V beta 17a+ T cells was mapped in these mice to the K end of H-2k, and H-2 influences on the level of selection of CD4+ V beta 17a+ T cells were indicated.

Full Text

The Full Text of this article is available as a PDF (582.0 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barth R. K., Kim B. S., Lan N. C., Hunkapiller T., Sobieck N., Winoto A., Gershenfeld H., Okada C., Hansburg D., Weissman I. L. The murine T-cell receptor uses a limited repertoire of expressed V beta gene segments. Nature. 1985 Aug 8;316(6028):517–523. doi: 10.1038/316517a0. [DOI] [PubMed] [Google Scholar]
  2. Behlke M. A., Chou H. S., Huppi K., Loh D. Y. Murine T-cell receptor mutants with deletions of beta-chain variable region genes. Proc Natl Acad Sci U S A. 1986 Feb;83(3):767–771. doi: 10.1073/pnas.83.3.767. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Behlke M. A., Spinella D. G., Chou H. S., Sha W., Hartl D. L., Loh D. Y. T-cell receptor beta-chain expression: dependence on relatively few variable region genes. Science. 1985 Aug 9;229(4713):566–570. doi: 10.1126/science.3875151. [DOI] [PubMed] [Google Scholar]
  4. Blackman M. A., Kappler J. W., Marrack P. T-cell specificity and repertoire. Immunol Rev. 1988 Jan;101:5–19. doi: 10.1111/j.1600-065x.1988.tb00730.x. [DOI] [PubMed] [Google Scholar]
  5. Carbone A. M., Marrack P., Kappler J. W. Remethylation at sites 5' of the murine Lyt-2 gene in association with shutdown of Lyt-2 expression. J Immunol. 1988 Aug 15;141(4):1369–1375. [PubMed] [Google Scholar]
  6. Chou H. S., Nelson C. A., Godambe S. A., Chaplin D. D., Loh D. Y. Germline organization of the murine T cell receptor beta-chain genes. Science. 1987 Oct 23;238(4826):545–548. doi: 10.1126/science.2821625. [DOI] [PubMed] [Google Scholar]
  7. Dialynas D. P., Quan Z. S., Wall K. A., Pierres A., Quintáns J., Loken M. R., Pierres M., Fitch F. W. Characterization of the murine T cell surface molecule, designated L3T4, identified by monoclonal antibody GK1.5: similarity of L3T4 to the human Leu-3/T4 molecule. J Immunol. 1983 Nov;131(5):2445–2451. [PubMed] [Google Scholar]
  8. Haskins K., Hannum C., White J., Roehm N., Kubo R., Kappler J., Marrack P. The antigen-specific, major histocompatibility complex-restricted receptor on T cells. VI. An antibody to a receptor allotype. J Exp Med. 1984 Aug 1;160(2):452–471. doi: 10.1084/jem.160.2.452. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Huppi K. E., D'Hoostelaere L. A., Mock B. A., Jouvin-Marche E., Behlke M. A., Chou H. S., Berry R. J., Loh D. Y. T-cell receptor VT beta genes in natural populations of mice. Immunogenetics. 1988;27(1):51–56. doi: 10.1007/BF00404444. [DOI] [PubMed] [Google Scholar]
  10. Julius M. H., Simpson E., Herzenberg L. A. A rapid method for the isolation of functional thymus-derived murine lymphocytes. Eur J Immunol. 1973 Oct;3(10):645–649. doi: 10.1002/eji.1830031011. [DOI] [PubMed] [Google Scholar]
  11. Kappler J. W., Marrack P. The role of H-2-linked genes in helper T-cell function. I. In vitro expression in B cells of immune response genes controlling helper T-cell activity. J Exp Med. 1977 Dec 1;146(6):1748–1764. doi: 10.1084/jem.146.6.1748. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kappler J. W., Roehm N., Marrack P. T cell tolerance by clonal elimination in the thymus. Cell. 1987 Apr 24;49(2):273–280. doi: 10.1016/0092-8674(87)90568-x. [DOI] [PubMed] [Google Scholar]
  13. Kappler J. W., Wade T., White J., Kushnir E., Blackman M., Bill J., Roehm N., Marrack P. A T cell receptor V beta segment that imparts reactivity to a class II major histocompatibility complex product. Cell. 1987 Apr 24;49(2):263–271. doi: 10.1016/0092-8674(87)90567-8. [DOI] [PubMed] [Google Scholar]
  14. Kisielow P., Blüthmann H., Staerz U. D., Steinmetz M., von Boehmer H. Tolerance in T-cell-receptor transgenic mice involves deletion of nonmature CD4+8+ thymocytes. Nature. 1988 Jun 23;333(6175):742–746. doi: 10.1038/333742a0. [DOI] [PubMed] [Google Scholar]
  15. Lai E., Barth R. K., Hood L. Genomic organization of the mouse T-cell receptor beta-chain gene family. Proc Natl Acad Sci U S A. 1987 Jun;84(11):3846–3850. doi: 10.1073/pnas.84.11.3846. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Ledbetter J. A., Herzenberg L. A. Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens. Immunol Rev. 1979;47:63–90. doi: 10.1111/j.1600-065x.1979.tb00289.x. [DOI] [PubMed] [Google Scholar]
  17. Lee N. E., Davis M. M. T cell receptor beta-chain genes in BW5147 and other AKR tumors. Deletion order of murine V beta gene segments and possible 5' regulatory regions. J Immunol. 1988 Mar 1;140(5):1665–1675. [PubMed] [Google Scholar]
  18. Oi V. T., Jones P. P., Goding J. W., Herzenberg L. A., Herzenberg L. A. Properties of monoclonal antibodies to mouse Ig allotypes, H-2, and Ia antigens. Curr Top Microbiol Immunol. 1978;81:115–120. doi: 10.1007/978-3-642-67448-8_18. [DOI] [PubMed] [Google Scholar]
  19. Ozato K., Mayer N. M., Sachs D. H. Monoclonal antibodies to mouse major histocompatibility complex antigens. Transplantation. 1982 Sep;34(3):113–120. doi: 10.1097/00007890-198209000-00001. [DOI] [PubMed] [Google Scholar]
  20. Ozato K., Sachs D. H. Monoclonal antibodies to mouse MHC antigens. III. Hybridoma antibodies reacting to antigens of the H-2b haplotype reveal genetic control of isotype expression. J Immunol. 1981 Jan;126(1):317–321. [PubMed] [Google Scholar]
  21. Pullen A. M., Marrack P., Kappler J. W. The T-cell repertoire is heavily influenced by tolerance to polymorphic self-antigens. Nature. 1988 Oct 27;335(6193):796–801. doi: 10.1038/335796a0. [DOI] [PubMed] [Google Scholar]
  22. Roehm N. W., Carbone A., Kushnir E., Taylor B. A., Riblet R. J., Marrack P., Kappler J. W. The major histocompatibility complex-restricted antigen receptor on T cells: the genetics of expression of an allotype. J Immunol. 1985 Sep;135(3):2176–2182. [PubMed] [Google Scholar]
  23. Singer P. A., McEvilly R. J., Noonan D. J., Dixon F. J., Theofilopoulos A. N. Clonal diversity and T-cell receptor beta-chain variable gene expression in enlarged lymph nodes of MRL-lpr/lpr lupus mice. Proc Natl Acad Sci U S A. 1986 Sep;83(18):7018–7022. doi: 10.1073/pnas.83.18.7018. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Wade T., Bill J., Marrack P. C., Palmer E., Kappler J. W. Molecular basis for the nonexpression of V beta 17 in some strains of mice. J Immunol. 1988 Sep 15;141(6):2165–2167. [PubMed] [Google Scholar]

Articles from The Journal of Experimental Medicine are provided here courtesy of The Rockefeller University Press

RESOURCES