Abstract
Curing of the hydrogenase-specifying megaplasmid pHG indigenous to strains of the facultative lithoautotrophic bacterium Alcaligenes eutrophus was correlated with a loss of denitrifying ability (Nitd). The retransfer of plasmid pHG1 reconstituted the Nitd phenotype. Plasmid-free mutants were still capable of converting some nitrate to nitrite, but they did not metabolize nitrite under anaerobic conditions.
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- Andersen K., Wilke-Douglas M. Construction and use of a gene bank of Alcaligenes eutrophus in the analysis of ribulose bisphosphate carboxylase genes. J Bacteriol. 1984 Sep;159(3):973–978. doi: 10.1128/jb.159.3.973-978.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Friedrich B., Friedrich C. G., Meyer M., Schlegel H. G. Expression of hydrogenase in Alcaligenes spp. is altered by interspecific plasmid exchange. J Bacteriol. 1984 Apr;158(1):331–333. doi: 10.1128/jb.158.1.331-333.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Friedrich B., Hogrefe C., Schlegel H. G. Naturally occurring genetic transfer of hydrogen-oxidizing ability between strains of Alcaligenes eutrophus. J Bacteriol. 1981 Jul;147(1):198–205. doi: 10.1128/jb.147.1.198-205.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hogrefe C., Römermann D., Friedrich B. Alcaligenes eutrophus hydrogenase genes (Hox). J Bacteriol. 1984 Apr;158(1):43–48. doi: 10.1128/jb.158.1.43-48.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LOWE R. H., EVANS H. J. PREPARATION AND SOME PROPERTIES OF A SOLUBLE NITRATE REDUCTASE FROM RHIZOBIUM JAPONICUM. Biochim Biophys Acta. 1964 Jun 1;85:377–389. doi: 10.1016/0926-6569(64)90301-3. [DOI] [PubMed] [Google Scholar]
- Pfitzner J., Schlegel H. G. Denitrifikation bei Hydrogenomonas eutropha Stamm H 16. Arch Mikrobiol. 1973;90(3):199–211. [PubMed] [Google Scholar]
- Probst I., Wolf G., Schlegel H. G. An oxygen-binding flavohemoprotein from Alcaligenes eutrophus. Biochim Biophys Acta. 1979 Feb 26;576(2):471–478. doi: 10.1016/0005-2795(79)90422-7. [DOI] [PubMed] [Google Scholar]