Abstract
Treatment of mice with a rat mAb (5C6) specific for an epitope of the type 3 complement receptor of myelomonocytic cells severely interfered with the ability of the mice to resist infection with Listeria monocytogenes. Consequently, a sublethal infection was rapidly converted to a lethal one that resulted in death in 5 d. However, infection was only exacerbated if 5C6 was given earlier in infection, before mononuclear phagocytes populated sites of Listeria implantation in the liver and spleen. If given after day 3 of infection, 5C6 caused only a temporary increase in bacterial multiplication. The infection- enhancing effect of 5C6 was associated with failure of mice to focus mononuclear phagocytes at sites of bacterial multiplication of Listeria in liver hepatocytes and extracellulary in the spleen. This resulted in unrestricted multiplication of Listeria in hepatocytes and extracellularly in the spleen. The results are in keeping with the ability of 5C6 to inhibit the accumulation of myelomonocytic cells in peritoneal inflammatory exudates, as revealed by a previous study.
Full Text
The Full Text of this article is available as a PDF (693.5 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Gaillard J. L., Berche P., Mounier J., Richard S., Sansonetti P. In vitro model of penetration and intracellular growth of Listeria monocytogenes in the human enterocyte-like cell line Caco-2. Infect Immun. 1987 Nov;55(11):2822–2829. doi: 10.1128/iai.55.11.2822-2829.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Havell E. A. Synthesis and secretion of interferon by murine fibroblasts in response to intracellular Listeria monocytogenes. Infect Immun. 1986 Dec;54(3):787–792. doi: 10.1128/iai.54.3.787-792.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kuhn M., Goebel W. Identification of an extracellular protein of Listeria monocytogenes possibly involved in intracellular uptake by mammalian cells. Infect Immun. 1989 Jan;57(1):55–61. doi: 10.1128/iai.57.1.55-61.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lee S. H., Crocker P., Gordon S. Macrophage plasma membrane and secretory properties in murine malaria. Effects of Plasmodium yoelii blood-stage infection on macrophages in liver, spleen, and blood. J Exp Med. 1986 Jan 1;163(1):54–74. doi: 10.1084/jem.163.1.54. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mackaness G. B. The influence of immunologically committed lymphoid cells on macrophage activity in vivo. J Exp Med. 1969 May 1;129(5):973–992. doi: 10.1084/jem.129.5.973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- North R. J. Cellular mediators of anti-Listeria immunity as an enlarged population of short lived, replicating T cells. Kinetics of their production. J Exp Med. 1973 Aug 1;138(2):342–355. doi: 10.1084/jem.138.2.342. [DOI] [PMC free article] [PubMed] [Google Scholar]
- North R. J., Deissler J. F. Nature of "memory" in T-cell mediated antibacterial immunity: cellular parameters that distinguish between the active immune response and a state of "memory". Infect Immun. 1975 Oct;12(4):761–767. doi: 10.1128/iai.12.4.761-767.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- North R. J. T cell dependence of macrophage activation and mobilization during infection with Mycobacterium tuberculosis. Infect Immun. 1974 Jul;10(1):66–71. doi: 10.1128/iai.10.1.66-71.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- North R. J. The relative importance of blood monocytes and fixed macrophages to the expression of cell-mediated immunity to infection. J Exp Med. 1970 Sep 1;132(3):521–534. doi: 10.1084/jem.132.3.521. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rosen H., Gordon S. Monoclonal antibody to the murine type 3 complement receptor inhibits adhesion of myelomonocytic cells in vitro and inflammatory cell recruitment in vivo. J Exp Med. 1987 Dec 1;166(6):1685–1701. doi: 10.1084/jem.166.6.1685. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rosen H., Milon G., Gordon S. Antibody to the murine type 3 complement receptor inhibits T lymphocyte-dependent recruitment of myelomonocytic cells in vivo. J Exp Med. 1989 Feb 1;169(2):535–548. doi: 10.1084/jem.169.2.535. [DOI] [PMC free article] [PubMed] [Google Scholar]
- van Dissel J. T., Stikkelbroeck J. J., van den Barselaar M. T., Sluiter W., Leijh P. C., van Furth R. Divergent changes in antimicrobial activity after immunologic activation of mouse peritoneal macrophages. J Immunol. 1987 Sep 1;139(5):1665–1672. [PubMed] [Google Scholar]