Abstract
We have raised an mAb to a previously undescribed 135-kD externally disposed integral membrane protein that is enriched in the intercellular junctional domain of cultured human umbilical vein endothelial cells. This protein localizes at the appositional surfaces of cells as they become confluent and is stably expressed in the junctional zones of confluent monolayers. This protein is expressed in situ on continuous endothelia of all blood vessels in all human tissues examined. Moreover, this protein, as determined by mAb immunocytochemistry, is not expressed by any other cell type. This protein may mediate endothelial-specific functions restricted to the intercellular domain. It may also serve as a unique cell surface marker for the identification and purification of human endothelial cells.
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- Edelman G. M. Cell adhesion molecules in the regulation of animal form and tissue pattern. Annu Rev Cell Biol. 1986;2:81–116. doi: 10.1146/annurev.cb.02.110186.000501. [DOI] [PubMed] [Google Scholar]
- Fitzgerald L. A., Charo I. F., Phillips D. R. Human and bovine endothelial cells synthesize membrane proteins similar to human platelet glycoproteins IIb and IIIa. J Biol Chem. 1985 Sep 15;260(20):10893–10896. [PubMed] [Google Scholar]
- Furie M. B., Cramer E. B., Naprstek B. L., Silverstein S. C. Cultured endothelial cell monolayers that restrict the transendothelial passage of macromolecules and electrical current. J Cell Biol. 1984 Mar;98(3):1033–1041. doi: 10.1083/jcb.98.3.1033. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ghandour S., Langley K., Gombos G., Hirn M., Hirsch M. R., Goridis C. A surface marker for murine vascular endothelial cells defined by monoclonal antibody. J Histochem Cytochem. 1982 Feb;30(2):165–170. doi: 10.1177/30.2.7061819. [DOI] [PubMed] [Google Scholar]
- Graham R. C., Jr, Karnovsky M. J. The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: ultrastructural cytochemistry by a new technique. J Histochem Cytochem. 1966 Apr;14(4):291–302. doi: 10.1177/14.4.291. [DOI] [PubMed] [Google Scholar]
- Holthöfer H., Virtanen I., Kariniemi A. L., Hormia M., Linder E., Miettinen A. Ulex europaeus I lectin as a marker for vascular endothelium in human tissues. Lab Invest. 1982 Jul;47(1):60–66. [PubMed] [Google Scholar]
- Hoyer L. W., De los Santos R. P., Hoyer J. R. Antihemophilic factor antigen. Localization in endothelial cells by immunofluorescent microscopy. J Clin Invest. 1973 Nov;52(11):2737–2744. doi: 10.1172/JCI107469. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jaffe E. A., Nachman R. L., Becker C. G., Minick C. R. Culture of human endothelial cells derived from umbilical veins. Identification by morphologic and immunologic criteria. J Clin Invest. 1973 Nov;52(11):2745–2756. doi: 10.1172/JCI107470. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kaplan K. L., Weber D., Cook P., Dalecki M., Rogozinski L., Sepe O., Knowles D., Butler V. P. Monoclonal antibodies to E92, an endothelial cell surface antigen. Arteriosclerosis. 1983 Sep-Oct;3(5):403–412. doi: 10.1161/01.atv.3.5.403. [DOI] [PubMed] [Google Scholar]
- Lane D. P., Lane E. B. A rapid antibody assay system for screening hybridoma cultures. J Immunol Methods. 1981;47(3):303–307. doi: 10.1016/0022-1759(81)90286-6. [DOI] [PubMed] [Google Scholar]
- Muller W. A., Gimbrone M. A., Jr Plasmalemmal proteins of cultured vascular endothelial cells exhibit apical-basal polarity: analysis by surface-selective iodination. J Cell Biol. 1986 Dec;103(6 Pt 1):2389–2402. doi: 10.1083/jcb.103.6.2389. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Muller W. A., Steinman R. M., Cohn Z. A. The membrane proteins of the vacuolar system I. Analysis of a novel method of intralysosomal iodination. J Cell Biol. 1980 Jul;86(1):292–303. doi: 10.1083/jcb.86.1.292. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Neville D. M., Jr Molecular weight determination of protein-dodecyl sulfate complexes by gel electrophoresis in a discontinuous buffer system. J Biol Chem. 1971 Oct 25;246(20):6328–6334. [PubMed] [Google Scholar]
- Newman P. J., Kawai Y., Montgomery R. R., Kunicki T. J. Synthesis by cultured human umbilical vein endothelial cells of two proteins structurally and immunologically related to platelet membrane glycoproteins IIb and IIIa. J Cell Biol. 1986 Jul;103(1):81–86. doi: 10.1083/jcb.103.1.81. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pawlowski N. A., Kaplan G., Abraham E., Cohn Z. A. The selective binding and transmigration of monocytes through the junctional complexes of human endothelium. J Exp Med. 1988 Nov 1;168(5):1865–1882. doi: 10.1084/jem.168.5.1865. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pober J. S., Gimbrone M. A., Jr Expression of Ia-like antigens by human vascular endothelial cells is inducible in vitro: demonstration by monoclonal antibody binding and immunoprecipitation. Proc Natl Acad Sci U S A. 1982 Nov;79(21):6641–6645. doi: 10.1073/pnas.79.21.6641. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schlingemann R. O., Dingjan G. M., Emeis J. J., Blok J., Warnaar S. O., Ruiter D. J. Monoclonal antibody PAL-E specific for endothelium. Lab Invest. 1985 Jan;52(1):71–76. [PubMed] [Google Scholar]
- Soffer R. L. Angiotensin-converting enzyme and the regulation of vasoactive peptides. Annu Rev Biochem. 1976;45:73–94. doi: 10.1146/annurev.bi.45.070176.000445. [DOI] [PubMed] [Google Scholar]
- Takeichi M. The cadherins: cell-cell adhesion molecules controlling animal morphogenesis. Development. 1988 Apr;102(4):639–655. doi: 10.1242/dev.102.4.639. [DOI] [PubMed] [Google Scholar]
- Volk T., Geiger B. A-CAM: a 135-kD receptor of intercellular adherens junctions. II. Antibody-mediated modulation of junction formation. J Cell Biol. 1986 Oct;103(4):1451–1464. doi: 10.1083/jcb.103.4.1451. [DOI] [PMC free article] [PubMed] [Google Scholar]