Abstract
B6 mice were treated in vivo with anti-CD4, anti-CD8, or both anti-T cell antibodies together in an effort to prolong xenogeneic compared with allogeneic skin graft survival. Mice treated with anti-CD4 antibody showed prolonged survival of xenogeneic monkey or rabbit skin even after they had rejected whole MHC-disparate allogeneic mouse skin. Furthermore, the addition of cyclosporine was synergistic with the anti- CD4 antibody in prolonging graft survival. These results suggest that the cell-mediated response to xenogeneic antigens is especially dependent on CD4+ lymphocytes, a feature shared by the response to allogeneic minor histocompatibility antigens. In addition, the results suggest a possible approach to clinical immunosuppression for some forms of xenogeneic transplantation.
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Selected References
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- Auchincloss H., Jr, Ghobrial R. R., Russell P. S., Winn H. J. Prevention of alloantibody formation after skin grafting without prolongation of graft survival by anti-L3T4 in vivo. Transplantation. 1988 Jun;45(6):1118–1123. doi: 10.1097/00007890-198806000-00024. [DOI] [PubMed] [Google Scholar]
- Auchincloss H., Jr, Mayer T., Ghobrial R., Winn H. J. T-cell subsets, bm mutants, and the mechanisms of allogeneic skin graft rejection. Immunol Res. 1989;8(2):149–164. doi: 10.1007/BF02919076. [DOI] [PubMed] [Google Scholar]
- Cobbold S. P., Jayasuriya A., Nash A., Prospero T. D., Waldmann H. Therapy with monoclonal antibodies by elimination of T-cell subsets in vivo. Nature. 1984 Dec 6;312(5994):548–551. doi: 10.1038/312548a0. [DOI] [PubMed] [Google Scholar]
- Cobbold S., Waldmann H. Skin allograft rejection by L3/T4+ and Lyt-2+ T cell subsets. Transplantation. 1986 May;41(5):634–639. doi: 10.1097/00007890-198605000-00016. [DOI] [PubMed] [Google Scholar]
- Dialynas D. P., Quan Z. S., Wall K. A., Pierres A., Quintáns J., Loken M. R., Pierres M., Fitch F. W. Characterization of the murine T cell surface molecule, designated L3T4, identified by monoclonal antibody GK1.5: similarity of L3T4 to the human Leu-3/T4 molecule. J Immunol. 1983 Nov;131(5):2445–2451. [PubMed] [Google Scholar]
- Greenstein J. L., Foran J. A., Gorga J. C., Burakoff S. J. The role of T cell accessory molecules in the generation of class II-specific xenogeneic cytolytic T cells. J Immunol. 1986 Apr 1;136(7):2358–2363. [PubMed] [Google Scholar]
- Ichikawa T., Nakayama E., Uenaka A., Monden M., Mori T. Effector cells in allelic H-2 class I-incompatible skin graft rejection. J Exp Med. 1987 Oct 1;166(4):982–990. doi: 10.1084/jem.166.4.982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jooste S. V., Winn H. J. Strain variations in the responses of mice to xenografts of skin. Transplant Proc. 1977 Mar;9(1):375–377. [PubMed] [Google Scholar]
- Mizuochi T., Golding H., Rosenberg A. S., Glimcher L. H., Malek T. R., Singer A. Both L3T4+ and Lyt-2+ helper T cells initiate cytotoxic T lymphocyte responses against allogenic major histocompatibility antigens but not against trinitrophenyl-modified self. J Exp Med. 1985 Aug 1;162(2):427–443. doi: 10.1084/jem.162.2.427. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sarmiento M., Glasebrook A. L., Fitch F. W. IgG or IgM monoclonal antibodies reactive with different determinants on the molecular complex bearing Lyt 2 antigen block T cell-mediated cytolysis in the absence of complement. J Immunol. 1980 Dec;125(6):2665–2672. [PubMed] [Google Scholar]
- Simpson E., Gordon R. D. Responsiveness to HY antigen Ir gene complementation and target cell specificity. Immunol Rev. 1977;35:59–75. doi: 10.1111/j.1600-065x.1977.tb00235.x. [DOI] [PubMed] [Google Scholar]
- Singer A., Munitz T. I., Golding H., Rosenberg A. S., Mizuochi T. Recognition requirements for the activation, differentiation and function of T-helper cells specific for class I MHC alloantigens. Immunol Rev. 1987 Aug;98:143–170. doi: 10.1111/j.1600-065x.1987.tb00523.x. [DOI] [PubMed] [Google Scholar]
- Wheelahan J., McKenzie I. F. The role of T4+ and Ly-2+ cells in skin graft rejection in the mouse. Transplantation. 1987 Aug;44(2):273–280. doi: 10.1097/00007890-198708000-00019. [DOI] [PubMed] [Google Scholar]
- Woodcock J., Wofsy D., Eriksson E., Scott J. H., Seaman W. E. Rejection of skin grafts and generation of cytotoxic T cells by mice depleted of L3T4+ cells. Transplantation. 1986 Dec;42(6):636–642. doi: 10.1097/00007890-198612000-00012. [DOI] [PubMed] [Google Scholar]