Abstract
We have found cell surface expression of an E alpha molecule in recombinant and transgenic mouse strains lacking an E beta molecule. Flow cytometry has shown low level expression of E alpha in B10.RQB3 (I- AqEk alpha) and B10.RFB2 (I-AfEk alpha) mice. We have also found that B10.Q (H-2q) mice can express the Ek alpha transgene. Since these strains do not have functional E beta chains, we propose that the E alpha A beta hybrid dimers are formed in low numbers and can be picked up by FACS analysis. So far we have not been able to identify these hybrid molecules by cytotoxicity or immunoprecipitation. The E alpha/A beta molecule can function in vivo during thymic selection in the clonal deletion of two V beta TCR subsets, V beta 11 and V beta 6, which have been shown to interact with the intact I-E molecule.
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Selected References
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- Begovich A. B., Jones P. P. Free Ia E alpha chain expression in the E+ alpha : E- beta recombinant strain A.TFR5. Immunogenetics. 1985;22(6):523–532. doi: 10.1007/BF00430300. [DOI] [PubMed] [Google Scholar]
- Dorf M. E., Plate J. M., Stimpfling J. H., Benacerraf B. Characterization of immune response and mixed lymphocyte reactions in selected intra-H-2 recombinant strains. J Immunol. 1975 Feb;114(2 Pt 1):602–605. [PubMed] [Google Scholar]
- Götze D., Rössler R., Thierfelder S. Other mammalian histocompatibility systems. Skin graft rejection and graft-versus-host reaction across I-subregion differences: are there many more than three H loci within the H-2 complex? Transplant Proc. 1977 Mar;9(1):547–550. [PubMed] [Google Scholar]
- Jones P. P., Murphy D. B., McDevitt H. O. Variable synthesis and expression of E alpha and Ae (E beta) Ia polypeptide chains in mice of different H-2 haplotypes. Immunogenetics. 1981;12(3-4):321–337. doi: 10.1007/BF01561674. [DOI] [PubMed] [Google Scholar]
- Kappler J. W., Roehm N., Marrack P. T cell tolerance by clonal elimination in the thymus. Cell. 1987 Apr 24;49(2):273–280. doi: 10.1016/0092-8674(87)90568-x. [DOI] [PubMed] [Google Scholar]
- Kappler J. W., Wade T., White J., Kushnir E., Blackman M., Bill J., Roehm N., Marrack P. A T cell receptor V beta segment that imparts reactivity to a class II major histocompatibility complex product. Cell. 1987 Apr 24;49(2):263–271. doi: 10.1016/0092-8674(87)90567-8. [DOI] [PubMed] [Google Scholar]
- Klein J., Geib R., Chiang C., Hauptfeld V. Histocompatibility antigens controlled by the I region of the murine H-2 complex. I. Mapping of H-2A and H-2C loci. J Exp Med. 1976 Jun 1;143(6):1439–1452. doi: 10.1084/jem.143.6.1439. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kupinski J. M., Gamble P. A., David C. S., Freed J. H. Biochemical documentation of allelic variation of the I-E antigens of the d, k, p, r, and u haplotypes. Immunogenetics. 1982;16(5):393–405. doi: 10.1007/BF00372099. [DOI] [PubMed] [Google Scholar]
- Lafuse W. P., David C. S. Recombination hot spots within the I region of the mouse H-2 complex map to the E beta and E alpha genes. Immunogenetics. 1986;24(6):352–360. doi: 10.1007/BF00377952. [DOI] [PubMed] [Google Scholar]
- Lafuse W. P., Savarirayan S., McCormick J. F., David C. S. Identification of I-E alpha genes in H-2 recombinant mouse strains by F1 complementation. Transplantation. 1987 Feb;43(2):297–301. doi: 10.1097/00007890-198702000-00026. [DOI] [PubMed] [Google Scholar]
- Livneh Z. Directed mutagenesis method for analysis of mutagen specificity: application to ultraviolet-induced mutagenesis. Proc Natl Acad Sci U S A. 1983 Jan;80(1):237–241. doi: 10.1073/pnas.80.1.237. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MacDonald H. R., Lees R. K., Schneider R., Zinkernagel R. M., Hengartner H. Positive selection of CD4+ thymocytes controlled by MHC class II gene products. Nature. 1988 Dec 1;336(6198):471–473. doi: 10.1038/336471a0. [DOI] [PubMed] [Google Scholar]
- Malissen B., Steinmetz M., McMillan M., Pierres M., Hood L. Expression of I-Ak class II genes in mouse L cells after DNA-mediated gene transfer. 1983 Sep 29-Oct 5Nature. 305(5933):440–443. doi: 10.1038/305440a0. [DOI] [PubMed] [Google Scholar]
- Murphy D. B., Jones P. P., Loken M. R., McDevitt H. O. Interaction between I region loci influences the expression of a cell surface Ia antigen. Proc Natl Acad Sci U S A. 1980 Sep;77(9):5404–5408. doi: 10.1073/pnas.77.9.5404. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Norcross M. A., Bentley D. M., Margulies D. H., Germain R. N. Membrane Ia expression and antigen-presenting accessory cell function of L cells transfected with class II major histocompatibility complex genes. J Exp Med. 1984 Nov 1;160(5):1316–1337. doi: 10.1084/jem.160.5.1316. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ozato K., Mayer N., Sachs D. H. Hybridoma cell lines secreting monoclonal antibodies to mouse H-2 and Ia antigens. J Immunol. 1980 Feb;124(2):533–540. [PubMed] [Google Scholar]
- Pullen A. M., Marrack P., Kappler J. W. The T-cell repertoire is heavily influenced by tolerance to polymorphic self-antigens. Nature. 1988 Oct 27;335(6193):796–801. doi: 10.1038/335796a0. [DOI] [PubMed] [Google Scholar]
- Spencer J. S., Kubo R. T. Mixed isotype class II antigen expression. A novel class II molecule is expressed on a murine B cell lymphoma. J Exp Med. 1989 Mar 1;169(3):625–640. doi: 10.1084/jem.169.3.625. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Steinmuller D. Skin grafting. Methods Enzymol. 1984;108:20–28. doi: 10.1016/s0076-6879(84)08070-8. [DOI] [PubMed] [Google Scholar]
- Tacchini-Cottier F. M., Jones P. P. Defective E beta expression in three mouse H-2 haplotypes results from aberrant RNA splicing. J Immunol. 1988 Nov 15;141(10):3647–3653. [PubMed] [Google Scholar]