Abstract
PWM-activated spleen cell-conditioned medium (SCCM) and a variety of purified hematopoietic growth factors were tested for their ability to stimulate chemotaxis of mouse connective tissue mast cells (CTMC). Of the agents tested, only IL-3 and SCCM promoted mast cell chemotaxis. Neither IL-2, IL-4, GM-CSF, nor endotoxin had any significant mast cell chemotactic activity. Neutralizing antibodies to mouse IL-3 blocked greater than 90% of the chemotactic activity of SCCM, suggesting that IL-3 is the predominant mast cell chemotactic factor produced by activated spleen cells. Our results demonstrate that mature connective tissue type mast cells are capable of moving toward a gradient of spleen cell-derived IL-3 and suggest that movement of mature mast cells toward lymphokines may influence the accumulation of mast cells at sites of inflammatory or immune reactions.
Full Text
The Full Text of this article is available as a PDF (333.6 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Abrams J. S., Pearce M. K. Development of rat anti-mouse interleukin 3 monoclonal antibodies which neutralize bioactivity in vitro. J Immunol. 1988 Jan 1;140(1):131–137. [PubMed] [Google Scholar]
- BOYDEN S. The chemotactic effect of mixtures of antibody and antigen on polymorphonuclear leucocytes. J Exp Med. 1962 Mar 1;115:453–466. doi: 10.1084/jem.115.3.453. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fox A. E., Plescia O. J. An experimental model of autoimmune hemolytic anemia in mice. Immunol Commun. 1973;2(3):241–254. doi: 10.3109/08820137309022796. [DOI] [PubMed] [Google Scholar]
- Galli S. J., Dvorak A. M., Dvorak H. F. Basophils and mast cells: morphologic insights into their biology, secretory patterns, and function. Prog Allergy. 1984;34:1–141. [PubMed] [Google Scholar]
- Grabstein K., Eisenman J., Mochizuki D., Shanebeck K., Conlon P., Hopp T., March C., Gillis S. Purification to homogeneity of B cell stimulating factor. A molecule that stimulates proliferation of multiple lymphokine-dependent cell lines. J Exp Med. 1986 Jun 1;163(6):1405–1414. doi: 10.1084/jem.163.6.1405. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hamaguchi Y., Kanakura Y., Fujita J., Takeda S., Nakano T., Tarui S., Honjo T., Kitamura Y. Interleukin 4 as an essential factor for in vitro clonal growth of murine connective tissue-type mast cells. J Exp Med. 1987 Jan 1;165(1):268–273. doi: 10.1084/jem.165.1.268. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lett-Brown M. A., Aelvoet M., Hooks J. J., Georgiades J. A., Thueson D. O., Grant J. A. Enhancement of basophil chemotaxis in vitro by virus-induced interferon. J Clin Invest. 1981 Feb;67(2):547–552. doi: 10.1172/JCI110065. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nakahata T., Ogawa M. Identification in culture of a class of hemopoietic colony-forming units with extensive capability to self-renew and generate multipotential hemopoietic colonies. Proc Natl Acad Sci U S A. 1982 Jun;79(12):3843–3847. doi: 10.1073/pnas.79.12.3843. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Orida N., Feldman J. D., Katz D. H., Liu F. T. IgE-mediated chemotaxis of rat basophilic leukemia cells towards specific antigen. J Exp Med. 1983 Jun 1;157(6):2166–2171. doi: 10.1084/jem.157.6.2166. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Poole T. J., Zetter B. R. Stimulation of rat peritoneal mast cell migration by tumor-derived peptides. Cancer Res. 1983 Dec;43(12 Pt 1):5857–5861. [PubMed] [Google Scholar]
- Prestidge R. L., Watson J. D., Urdal D. L., Mochizuki D., Conlon P., Gillis S. Biochemical comparison of murine colony-stimulating factors secreted by a T cell lymphoma and a myelomonocytic leukemia. J Immunol. 1984 Jul;133(1):293–298. [PubMed] [Google Scholar]
- Zigmond S. H., Hirsch J. G. Leukocyte locomotion and chemotaxis. New methods for evaluation, and demonstration of a cell-derived chemotactic factor. J Exp Med. 1973 Feb 1;137(2):387–410. doi: 10.1084/jem.137.2.387. [DOI] [PMC free article] [PubMed] [Google Scholar]