Abstract
CSF-1 and granulocyte/monocyte CSF (GM-CSF) were shown to modulate the levels of Ia gene and protein expression in bone marrow-derived macrophages (BMM). Recombinant GM-CSF induced high levels of Ia expression, similar to the levels induced by INF-gamma, while IL-3 had no effect. In contrast, recombinant CSF-1 not only suppressed the basal levels of Ia gene and protein expression in BMM, but also inhibited the induction of Ia by IFN-gamma and GM-CSF. Basal levels of Ia were not inhibited by recombinant CSF-1 until after 16-24 h of culture, suggesting an indirect mechanism of suppression. IFN-alpha/beta and PGE2 were shown not to be involved in the CSF-1 inhibition of basal levels of Ia expression. However, the CSF-1-mediated suppression of both the basal levels of Ia expression and the induction of Ia in BMM by IFN-gamma and GM-CSF did correlate with the induction of cellular proliferation. These data imply that in addition to regulating hematopoiesis, CSFs may regulate the initiation of the immune response through their effects on Ia expression in macrophages.
Full Text
The Full Text of this article is available as a PDF (675.9 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Calamai E. G., Beller D. I., Unanue E. R. Regulation of macrophage populations. IV. Modulation of Ia expression in bone marrow-derived macrophages. J Immunol. 1982 Apr;128(4):1692–1694. [PubMed] [Google Scholar]
- Crawford R. M., Finbloom D. S., Ohara J., Paul W. E., Meltzer M. S. B cell stimulatory factor-1 (interleukin 4) activates macrophages for increased tumoricidal activity and expression of Ia antigens. J Immunol. 1987 Jul 1;139(1):135–141. [PubMed] [Google Scholar]
- Falk L. A., Wahl L. M., Vogel S. N. Analysis of Ia antigen expression in macrophages derived from bone marrow cells cultured in granulocyte-macrophage colony-stimulating factor or macrophage colony-stimulating factor. J Immunol. 1988 Apr 15;140(8):2652–2660. [PubMed] [Google Scholar]
- Fertsch-Ruggio D., Schoenberg D. R., Vogel S. N. Induction of macrophage Ia antigen expression by rIFN-gamma and down-regulation by IFN-alpha/beta and dexamethasone are regulated transcriptionally. J Immunol. 1988 Sep 1;141(5):1582–1589. [PubMed] [Google Scholar]
- King D. P., Jones P. P. Induction of Ia and H-2 antigens on a macrophage cell line by immune interferon. J Immunol. 1983 Jul;131(1):315–318. [PubMed] [Google Scholar]
- Koerner T. J., Hamilton T. A., Adams D. O. Suppressed expression of surface Ia on macrophages by lipopolysaccharide: evidence for regulation at the level of accumulation of mRNA. J Immunol. 1987 Jul 1;139(1):239–243. [PubMed] [Google Scholar]
- Ling P. D., Warren M. K., Vogel S. N. Antagonistic effect of interferon-beta on the interferon-gamma-induced expression of Ia antigen in murine macrophages. J Immunol. 1985 Sep;135(3):1857–1863. [PubMed] [Google Scholar]
- Malissen M., Hunkapiller T., Hood L. Nucleotide sequence of a light chain gene of the mouse I-A subregion: A beta d. Science. 1983 Aug 19;221(4612):750–754. doi: 10.1126/science.6410508. [DOI] [PubMed] [Google Scholar]
- Moore R. N., Pitruzzello F. J., Robinson R. M., Rouse B. T. Interferon produced endogenously in response to CSF-1 augments the functional differentiation of progeny macrophages. J Leukoc Biol. 1985 May;37(5):659–664. doi: 10.1002/jlb.37.5.659. [DOI] [PubMed] [Google Scholar]
- Morrissey P. J., Bressler L., Park L. S., Alpert A., Gillis S. Granulocyte-macrophage colony-stimulating factor augments the primary antibody response by enhancing the function of antigen-presenting cells. J Immunol. 1987 Aug 15;139(4):1113–1119. [PubMed] [Google Scholar]
- Singer P. A., Lauer W., Dembić Z., Mayer W. E., Lipp J., Koch N., Hämmerling G., Klein J., Dobberstein B. Structure of the murine Ia-associated invariant (Ii) chain as deduced from a cDNA clone. EMBO J. 1984 Apr;3(4):873–877. doi: 10.1002/j.1460-2075.1984.tb01899.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Steinmetz M., Moore K. W., Frelinger J. G., Sher B. T., Shen F. W., Boyse E. A., Hood L. A pseudogene homologous to mouse transplantation antigens: transplantation antigens are encoded by eight exons that correlate with protein domains. Cell. 1981 Sep;25(3):683–692. doi: 10.1016/0092-8674(81)90175-6. [DOI] [PubMed] [Google Scholar]
- Unanue E. R., Allen P. M. The basis for the immunoregulatory role of macrophages and other accessory cells. Science. 1987 May 1;236(4801):551–557. doi: 10.1126/science.2437650. [DOI] [PubMed] [Google Scholar]
- Willman C. L., Stewart C. C., Griffith J. K., Stewart S. J., Tomasi T. B. Differential expression and regulation of the c-src and c-fgr protooncogenes in myelomonocytic cells. Proc Natl Acad Sci U S A. 1987 Jul;84(13):4480–4484. doi: 10.1073/pnas.84.13.4480. [DOI] [PMC free article] [PubMed] [Google Scholar]