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. 1989 Nov 1;170(5):1745–1750. doi: 10.1084/jem.170.5.1745

Effects of the neutrophil-activating peptide NAP-2, platelet basic protein, connective tissue-activating peptide III and platelet factor 4 on human neutrophils

PMCID: PMC2189513  PMID: 2681518

Abstract

Platelet basic protein (PBP), connective tissue-activating peptide III (CTAP-III), and platelet factor 4 (PF-4) were purified from human platelet release supernatants by heparin-Sepharose ion-exchange and reversed-phase HPLC, and their neutrophil-activating effects were compared with those of NAP-2, a peptide of 70 amino acids corresponding to part of the sequence of PBP (1) and with sequence homology to NAF/NAP-1. NAP-2-induced elastase release and a rise in cytosolic free Ca2+ at concentrations between 0.3 and 100 nM, and neutrophil chemotaxis at concentrations between 0.03 and 10 nM. It was half as potent as NAF/NAP-1 in inducing exocytosis but showed the same activity in the other responses. By contrast, only minimal if any effects were obtained with PBP, CTAP-III, and PF-4 up to 100 nM. NAP-2 thus appears to behave like a typical chemotactic receptor agonist. It could be generated from PBP and/or CTAP-III released from activated platelets and lead to the accumulation of neutrophils in platelet aggregates.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baggiolini M., Dewald B., Thelen M. Effects of PAF on neutrophils and mononuclear phagocytes. Prog Biochem Pharmacol. 1988;22:90–105. [PubMed] [Google Scholar]
  2. Bebawy S. T., Gorka J., Hyers T. M., Webster R. O. In vitro effects of platelet factor 4 on normal human neutrophil functions. J Leukoc Biol. 1986 Apr;39(4):423–434. doi: 10.1002/jlb.39.4.423. [DOI] [PubMed] [Google Scholar]
  3. Bienz D., Clemetson K. J. Human platelet glycoprotein Ia. One component is only expressed on the surface of activated platelets and may be a granule constituent. J Biol Chem. 1989 Jan 5;264(1):507–514. [PubMed] [Google Scholar]
  4. Braunstein P. W., Cuénoud H. F., Joris I., Majno G. Platelets, fibroblasts, and inflammation: tissue reactions to platelets injected subcutaneously. Am J Pathol. 1980 Apr;99(1):53–66. [PMC free article] [PubMed] [Google Scholar]
  5. Castor C. W., Miller J. W., Walz D. A. Structural and biological characteristics of connective tissue activating peptide (CTAP-III), a major human platelet-derived growth factor. Proc Natl Acad Sci U S A. 1983 Feb;80(3):765–769. doi: 10.1073/pnas.80.3.765. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Castor C. W., Ritchie J. C., Williams C. H., Jr, Scott M. E., Whitney S. L., Myers S. L., Sloan T. B., Anderson B. E. Connective tissue activation. XIV. Composition and actions of a human platelet autacoid mediator. Arthritis Rheum. 1979 Mar;22(3):260–272. doi: 10.1002/art.1780220308. [DOI] [PubMed] [Google Scholar]
  7. Deuel T. F., Keim P. S., Farmer M., Heinrikson R. L. Amino acid sequence of human platelet factor 4. Proc Natl Acad Sci U S A. 1977 Jun;74(6):2256–2258. doi: 10.1073/pnas.74.6.2256. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Deuel T. F., Senior R. M., Chang D., Griffin G. L., Heinrikson R. L., Kaiser E. T. Platelet factor 4 is chemotactic for neutrophils and monocytes. Proc Natl Acad Sci U S A. 1981 Jul;78(7):4584–4587. doi: 10.1073/pnas.78.7.4584. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Holt J. C., Harris M. E., Holt A. M., Lange E., Henschen A., Niewiarowski S. Characterization of human platelet basic protein, a precursor form of low-affinity platelet factor 4 and beta-thromboglobulin. Biochemistry. 1986 Apr 22;25(8):1988–1996. doi: 10.1021/bi00356a023. [DOI] [PubMed] [Google Scholar]
  10. Peveri P., Walz A., Dewald B., Baggiolini M. A novel neutrophil-activating factor produced by human mononuclear phagocytes. J Exp Med. 1988 May 1;167(5):1547–1559. doi: 10.1084/jem.167.5.1547. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Pinckard R. N., Halonen M., Palmer J. D., Butler C., Shaw J. O., Henson P. M. Intravascular aggregation and pulmonary sequestration of platelets during IgE-induced systemic anaphylaxis in the rabbit: abrogation of lethal anaphylactic shock by platelet depletion. J Immunol. 1977 Dec;119(6):2185–2193. [PubMed] [Google Scholar]
  12. Schröder J. M., Mrowietz U., Morita E., Christophers E. Purification and partial biochemical characterization of a human monocyte-derived, neutrophil-activating peptide that lacks interleukin 1 activity. J Immunol. 1987 Nov 15;139(10):3474–3483. [PubMed] [Google Scholar]
  13. Senior R. M., Griffin G. L., Huang J. S., Walz D. A., Deuel T. F. Chemotactic activity of platelet alpha granule proteins for fibroblasts. J Cell Biol. 1983 Feb;96(2):382–385. doi: 10.1083/jcb.96.2.382. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Walz A., Baggiolini M. A novel cleavage product of beta-thromboglobulin formed in cultures of stimulated mononuclear cells activates human neutrophils. Biochem Biophys Res Commun. 1989 Mar 31;159(3):969–975. doi: 10.1016/0006-291x(89)92203-1. [DOI] [PubMed] [Google Scholar]
  15. Westwick J., Li S. W., Camp R. D. Novel neutrophil-stimulating peptides. Immunol Today. 1989 May;10(5):146–147. doi: 10.1016/0167-5699(89)90164-3. [DOI] [PubMed] [Google Scholar]
  16. Wolpe S. D., Sherry B., Juers D., Davatelis G., Yurt R. W., Cerami A. Identification and characterization of macrophage inflammatory protein 2. Proc Natl Acad Sci U S A. 1989 Jan;86(2):612–616. doi: 10.1073/pnas.86.2.612. [DOI] [PMC free article] [PubMed] [Google Scholar]

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