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. 1985 Apr;162(1):55–60. doi: 10.1128/jb.162.1.55-60.1985

5-Methyl-2-thiouridine in the tRNA of Candida tropicalis and its localization in lysine tRNA.

S Patwardhan, J D Cherayil
PMCID: PMC218952  PMID: 3845081

Abstract

35S incorporation studies showed that Candida tropicalis tRNA contained two thionucleosides, one of which was identified as 5-methyl-2-thiouridine. The other thionucleoside was alkali labile, and it appeared to be an ester. Pulse-chase experiments suggested that the two thionucleosides were structurally related. 5-Methyl-2-thiouridine was present in one of the lysine tRNAs. This is the first report of the presence of this nucleoside in a yeast tRNA.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Carbon J., David H. Thiobases in escherichia coli transfer RNA: 2-thiocytosine and 5-methylaminomethyl-2-thiouracil. Science. 1967 Sep 13;161(3846):1146–1147. [PubMed] [Google Scholar]
  2. Chan J. C., Yang J. A., Dunn M. J., Agris P. F., Wong T. W. The nucleotide sequence of a glutamine tRNA from rat liver. Nucleic Acids Res. 1982 Jun 25;10(12):3755–3758. doi: 10.1093/nar/10.12.3755. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Gauss D. H., Sprinzl M. Compilation of tRNA sequences. Nucleic Acids Res. 1983 Jan 11;11(1):r1–53. [PMC free article] [PubMed] [Google Scholar]
  4. Gezelius G. A convenient, efficient, and inexpensive method for scintillation counting of polyacrylamide gel slices after electrophoresis. Anal Biochem. 1977 Jun;80(2):627–634. doi: 10.1016/0003-2697(77)90688-1. [DOI] [PubMed] [Google Scholar]
  5. Gillam I., Millward S., Blew D., von Tigerstrom M., Wimmer E., Tener G. M. The separation of soluble ribonucleic acids on benzoylated diethylaminoethylcellulose. Biochemistry. 1967 Oct;6(10):3043–3056. doi: 10.1021/bi00862a011. [DOI] [PubMed] [Google Scholar]
  6. Johnson P. F., Abelson J. The yeast tRNATyr gene intron is essential for correct modification of its tRNA product. Nature. 1983 Apr 21;302(5910):681–687. doi: 10.1038/302681a0. [DOI] [PubMed] [Google Scholar]
  7. Kimura-Harada F., Saneyoshi M., Nishimura S. 5-methyl-2-thiouridine: A new sulfur-containing minor constituent from rat liver glutamic acid and lysine tRNAs. FEBS Lett. 1971 Apr 2;13(6):335–338. doi: 10.1016/0014-5793(71)80254-5. [DOI] [PubMed] [Google Scholar]
  8. Kobayashi T., Irie T., Yoshida M., Takeishi K., Ukita T. The primary structure of yeast glutamic acid tRNA specific to the GAA codon. Biochim Biophys Acta. 1974 Oct 11;366(2):168–181. doi: 10.1016/0005-2787(74)90331-1. [DOI] [PubMed] [Google Scholar]
  9. Madison J. T., Boguslawski S. J., Teetor G. H. Oligonucleotide composition of a yeast lysine transfer ribonucleic acid. Biochemistry. 1974 Jan 29;13(3):518–523. doi: 10.1021/bi00700a018. [DOI] [PubMed] [Google Scholar]
  10. Pearson R. L., Weiss J. F., Kelmers A. D. Improved separation of transfer RNA's on polychlorotrifuoroethylene-supported reversed-phase chromatography columns. Biochim Biophys Acta. 1971 Feb 11;228(3):770–774. doi: 10.1016/0005-2787(71)90748-9. [DOI] [PubMed] [Google Scholar]
  11. Raba M., Limburg K., Burghagen M., Katze J. R., Simsek M., Heckman J. E., Rajbhandary U. L., Gross H. J. Nucleotide sequence of three isoaccepting lysine tRNAs from rabbit liver and SV40-transformed mouse fibroblasts. Eur J Biochem. 1979 Jun;97(1):305–318. doi: 10.1111/j.1432-1033.1979.tb13115.x. [DOI] [PubMed] [Google Scholar]
  12. Rao Y. S., Cherayil J. D. Separation of 35 S-labeled thionucleosides of Escherichia coli and Pseudomonas aeruginosa transfer RNAs on a phosphocellulose column. Biochim Biophys Acta. 1973 Feb 23;299(1):1–7. doi: 10.1016/0005-2787(73)90391-2. [DOI] [PubMed] [Google Scholar]
  13. Saneyoshi M., Oashi Z., Harada F., Nishimura S. Isolation and characterization of 2-methyladenosine from Escherichia coli tRNA Glu 2 , tRNA Asp 1 , tRNA His 1 and tRNA Arg . Biochim Biophys Acta. 1972 Feb 23;262(1):1–10. [PubMed] [Google Scholar]
  14. Watanabe K., Kuchino Y., Yamaizumi Z., Kato M., Oshima T., Nishimura S. Nucleotide sequence of formylmethionine tRNA from an extreme thermophile, Thermus thermophilus HB8. J Biochem. 1979 Oct;86(4):893–905. doi: 10.1093/oxfordjournals.jbchem.a132621. [DOI] [PubMed] [Google Scholar]
  15. Wong T. W., McCutchan T., Kohli J., Söll D. The nucleotide sequence of the major glutamate transfer RNA from Schizosaccharomyces pombe. Nucleic Acids Res. 1979;6(6):2057–2068. doi: 10.1093/nar/6.6.2057. [DOI] [PMC free article] [PubMed] [Google Scholar]

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