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. 1987 Aug 1;166(2):327–340. doi: 10.1084/jem.166.2.327

Fine mapping of epitopes by intradomain Kd/Dd recombinants

PMCID: PMC2189604  PMID: 2439641

Abstract

11 intradomain recombinants between H-2Kd and H-2Dd were produced using an original technique based on in vivo recombination in Escherichia coli. After transfection into mouse L cells, all these recombinants were expressed at high levels on the cell surface. The specificities of 77 mAbs were examined on these cell lines. mAbs could be organized in 12 groups. In each group, a small number of amino acids participating in the recognized epitope(s) were identified. In a few instances, noncontinuous epitopes comprising amino acids belonging to different domains of the antigen were found. The data thus obtained are compatible with those produced in previous exon-shuffling experiments, but permit a much more precise definition of recognized epitope(s).

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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