Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1985 Apr;162(1):106–109. doi: 10.1128/jb.162.1.106-109.1985

Escherichia coli mutants possessing an Li+-resistant melibiose carrier.

S Shiota, Y Yamane, M Futai, T Tsuchiya
PMCID: PMC218961  PMID: 3884586

Abstract

Escherichia coli K-12 strains in the absence of the lactose carrier grew on the disaccharide melibiose as the sole source of carbon. The presence of 0.1 mM Li+ in the medium strongly inhibited growth of such cells, and Li+-resistant mutants appeared after several days of incubation. These mutants showed altered cation coupling to melibiose transport via the melibiose carrier. Cotransport between H+ and melibiose was lost in the mutants, although Na+-melibiose cotransport was retained. We observed no Li+-melibiose cotransport. Therefore, these mutants represent a new type of cation-coupling mutants of the melibiose carrier.

Full text

PDF
106

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  2. Lopilato J., Tsuchiya T., Wilson T. H. Role of Na+ and Li+ in thiomethylgalactoside transport by the melibiose transport system of Escherichia coli. J Bacteriol. 1978 Apr;134(1):147–156. doi: 10.1128/jb.134.1.147-156.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Niiya S., Yamasaki K., Wilson T. H., Tsuchiya T. Altered cation coupling to melibiose transport in mutants of Escherichia coli. J Biol Chem. 1982 Aug 10;257(15):8902–8906. [PubMed] [Google Scholar]
  4. Schuldiner S., Fishkes H. Sodium-proton antiport in isolated membrane vesicles of Escherichia coli. Biochemistry. 1978 Feb 21;17(4):706–711. doi: 10.1021/bi00597a023. [DOI] [PubMed] [Google Scholar]
  5. Shiota S., Yazyu H., Tsuchiya T. Escherichia coli mutants with altered cation recognition by the melibiose carrier. J Bacteriol. 1984 Oct;160(1):445–447. doi: 10.1128/jb.160.1.445-447.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Stock J., Roseman S. A sodium-dependent sugar co-transport system in bacteria. Biochem Biophys Res Commun. 1971 Jul 2;44(1):132–138. doi: 10.1016/s0006-291x(71)80168-7. [DOI] [PubMed] [Google Scholar]
  7. Tanaka K., Niiya S., Tsuchiya T. Melibiose transport of Escherichia coli. J Bacteriol. 1980 Mar;141(3):1031–1036. doi: 10.1128/jb.141.3.1031-1036.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Tanaka S., Lerner S. A., Lin E. C. Replacement of a phosphoenolpyruvate-dependent phosphotransferase by a nicotinamide adenine dinucleotide-linked dehydrogenase for the utilization of mannitol. J Bacteriol. 1967 Feb;93(2):642–648. doi: 10.1128/jb.93.2.642-648.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Tsuchiya T., Lopilato J., Wilson T. H. Effect of lithium ion on melibiose transport in Escherichia coli. J Membr Biol. 1978 Jul 21;42(1):45–59. doi: 10.1007/BF01870393. [DOI] [PubMed] [Google Scholar]
  10. Tsuchiya T., Oho M., Shiota-Niiya S. Lithium ion-sugar cotransport via the melibiose transport system in Escherichia coli. Measurement of Li+ transport and specificity. J Biol Chem. 1983 Nov 10;258(21):12765–12767. [PubMed] [Google Scholar]
  11. Tsuchiya T., Raven J., Wilson T. H. Co-transport of Na+ and methul-beta-D-thiogalactopyranoside mediated by the melibiose transport system of Escherichia coli. Biochem Biophys Res Commun. 1977 May 9;76(1):26–31. doi: 10.1016/0006-291x(77)91663-1. [DOI] [PubMed] [Google Scholar]
  12. Tsuchiya T., Wilson T. H. Cation-sugar cotransport in the melibiose transport system of Escherichia coli. Membr Biochem. 1978;2(1):63–79. doi: 10.3109/09687687809063858. [DOI] [PubMed] [Google Scholar]
  13. Umeda K., Shiota S., Futai M., Tsuchiya T. Inhibitory effect of Li+ on cell growth and pyruvate kinase activity of Escherichia coli. J Bacteriol. 1984 Nov;160(2):812–814. doi: 10.1128/jb.160.2.812-814.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. West I. C., Wilson T. H. Galactoside transport dissociated from proton movement in mutants of Escherichia coli. Biochem Biophys Res Commun. 1973 Jan 23;50(2):551–558. doi: 10.1016/0006-291x(73)90875-9. [DOI] [PubMed] [Google Scholar]
  15. Wilson T. H., Kusch M. A mutant of Escherichia coli K 12 energy-uncoupled for lactose transport. Biochim Biophys Acta. 1972 Mar 17;255(3):786–797. doi: 10.1016/0005-2736(72)90391-4. [DOI] [PubMed] [Google Scholar]
  16. Yazyu H., Shiota-Niiya S., Shimamoto T., Kanazawa H., Futai M., Tsuchiya T. Nucleotide sequence of the melB gene and characteristics of deduced amino acid sequence of the melibiose carrier in Escherichia coli. J Biol Chem. 1984 Apr 10;259(7):4320–4326. [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES