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. 1987 Nov 1;166(5):1245–1258. doi: 10.1084/jem.166.5.1245

Thymocyte clones from 14-day mouse embryos. I. State of T cell receptor genes, surface markers, and growth requirements

PMCID: PMC2189657  PMID: 3500258

Abstract

We have established in culture 13 clones from the thymus of a 14-d B10.BR mouse embryo and characterized 8 of them. All eight FT clones have the TCR-gamma and -beta genes in germline configuration. They express mRNA for the gamma, but not for the beta nor the alpha genes. All eight FT clones are Thy-1+, Ly-1+, LFA-1+, Pgp-1+, H-2K+, and T3-. Three phenotypes could be distinguished on the basis of Lyt-2, L3T4, and IL-2-R expression: Lyt-2+, L3T4-, IL-2-R+ (I); Lyt-2+, L3T4-, IL-2- R- (II); and Lyt-2+, L3T4+, IL-2-R+ (III) cells. All eight clones grow in rIL-4 and six clones also proliferate in rIL-2. Antibodies specific for IL-2-R inhibit their response to rIL-2 but not to rIL-4. The eight FT clones synthesize mRNA for IL-4 after stimulation in vitro and none of them exhibit cytolytic activity or helper function for B lymphocytes. We conclude that the FT clones are at a very early stage of T cell development, that the expression of Lyt-2 and L3T4 surface molecules can precede that of the antigen receptor, and that the same fetal thymocyte can use both IL-4 and IL-2 as growth factor.

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Selected References

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  1. Bank I., DePinho R. A., Brenner M. B., Cassimeris J., Alt F. W., Chess L. A functional T3 molecule associated with a novel heterodimer on the surface of immature human thymocytes. Nature. 1986 Jul 10;322(6075):179–181. doi: 10.1038/322179a0. [DOI] [PubMed] [Google Scholar]
  2. Born W., Rathbun G., Tucker P., Marrack P., Kappler J. Synchronized rearrangement of T-cell gamma and beta chain genes in fetal thymocyte development. Science. 1986 Oct 24;234(4775):479–482. doi: 10.1126/science.3020688. [DOI] [PubMed] [Google Scholar]
  3. Brenner M. B., McLean J., Dialynas D. P., Strominger J. L., Smith J. A., Owen F. L., Seidman J. G., Ip S., Rosen F., Krangel M. S. Identification of a putative second T-cell receptor. Nature. 1986 Jul 10;322(6075):145–149. doi: 10.1038/322145a0. [DOI] [PubMed] [Google Scholar]
  4. Ceredig R., Dialynas D. P., Fitch F. W., MacDonald H. R. Precursors of T cell growth factor producing cells in the thymus: ontogeny, frequency, and quantitative recovery in a subpopulation of phenotypically mature thymocytes defined by monoclonal antibody GK-1.5. J Exp Med. 1983 Nov 1;158(5):1654–1671. doi: 10.1084/jem.158.5.1654. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Dembić Z., Bannwarth W., Taylor B. A., Steinmetz M. The gene encoding the T-cell receptor alpha-chain maps close to the Np-2 locus on mouse chromosome 14. Nature. 1985 Mar 21;314(6008):271–273. doi: 10.1038/314271a0. [DOI] [PubMed] [Google Scholar]
  6. Dembić Z., Haas W., Weiss S., McCubrey J., Kiefer H., von Boehmer H., Steinmetz M. Transfer of specificity by murine alpha and beta T-cell receptor genes. Nature. 1986 Mar 20;320(6059):232–238. doi: 10.1038/320232a0. [DOI] [PubMed] [Google Scholar]
  7. Devos R., Plaetinck G., Cheroutre H., Simons G., Degrave W., Tavernier J., Remaut E., Fiers W. Molecular cloning of human interleukin 2 cDNA and its expression in E. coli. Nucleic Acids Res. 1983 Jul 11;11(13):4307–4323. doi: 10.1093/nar/11.13.4307. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Garman R. D., Doherty P. J., Raulet D. H. Diversity, rearrangement, and expression of murine T cell gamma genes. Cell. 1986 Jun 6;45(5):733–742. doi: 10.1016/0092-8674(86)90787-7. [DOI] [PubMed] [Google Scholar]
  9. Kisielow P., Leiserson W., Von Boehmer H. Differentiation of thymocytes in fetal organ culture: analysis of phenotypic changes accompanying the appearance of cytolytic and interleukin 2-producing cells. J Immunol. 1984 Sep;133(3):1117–1123. [PubMed] [Google Scholar]
  10. Kronenberg M., Siu G., Hood L. E., Shastri N. The molecular genetics of the T-cell antigen receptor and T-cell antigen recognition. Annu Rev Immunol. 1986;4:529–591. doi: 10.1146/annurev.iy.04.040186.002525. [DOI] [PubMed] [Google Scholar]
  11. Leo O., Foo M., Sachs D. H., Samelson L. E., Bluestone J. A. Identification of a monoclonal antibody specific for a murine T3 polypeptide. Proc Natl Acad Sci U S A. 1987 Mar;84(5):1374–1378. doi: 10.1073/pnas.84.5.1374. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Lowenthal J. W., Howe R. C., Ceredig R., MacDonald H. R. Functional status of interleukin 2 receptors expressed by immature (Lyt-2-/L3T4-) thymocytes. J Immunol. 1986 Oct 15;137(8):2579–2584. [PubMed] [Google Scholar]
  13. Mathieson B. J., Fowlkes B. J. Cell surface antigen expression on thymocytes: development and phenotypic differentiation of intrathymic subsets. Immunol Rev. 1984 Dec;82:141–173. doi: 10.1111/j.1600-065x.1984.tb01121.x. [DOI] [PubMed] [Google Scholar]
  14. Moingeon P., Ythier A., Goubin G., Faure F., Nowill A., Delmon L., Rainaud M., Forestier F., Daffos F., Bohuon C. A unique T-cell receptor complex expressed on human fetal lymphocytes displaying natural-killer-like activity. Nature. 1986 Oct 16;323(6089):638–640. doi: 10.1038/323638a0. [DOI] [PubMed] [Google Scholar]
  15. Noma Y., Sideras P., Naito T., Bergstedt-Lindquist S., Azuma C., Severinson E., Tanabe T., Kinashi T., Matsuda F., Yaoita Y. Cloning of cDNA encoding the murine IgG1 induction factor by a novel strategy using SP6 promoter. Nature. 1986 Feb 20;319(6055):640–646. doi: 10.1038/319640a0. [DOI] [PubMed] [Google Scholar]
  16. Palacios R., Kiefer M., Brockhaus M., Karjalainen K., Dembić Z., Kisielow P., von Boehmer H. Molecular, cellular, and functional properties of bone marrow T lymphocyte progenitor clones. J Exp Med. 1987 Jul 1;166(1):12–32. doi: 10.1084/jem.166.1.12. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Palacios R., Leu T. CC11: a monoclonal antibody specific for interleukin 3-sensitive mouse cells defines two major populations of B cell precursors in the bone marrow. Immunol Rev. 1986 Oct;93:125–146. doi: 10.1111/j.1600-065x.1986.tb01505.x. [DOI] [PubMed] [Google Scholar]
  18. Palacios R. Spontaneous production of interleukin 3 by T lymphocytes from autoimmune MRL/MP-lpr/lpr mice. Eur J Immunol. 1984 Jul;14(7):599–605. doi: 10.1002/eji.1830140704. [DOI] [PubMed] [Google Scholar]
  19. Palacios R., Von Boehmer H. Requirements for growth of immature thymocytes from fetal and adult mice in vitro. Eur J Immunol. 1986 Jan;16(1):12–19. doi: 10.1002/eji.1830160104. [DOI] [PubMed] [Google Scholar]
  20. Pelkonen J., Traunecker A., Karjalainen K. A new mouse TCR V gamma gene that shows remarkable evolutionary conservation. EMBO J. 1987 Jul;6(7):1941–1944. doi: 10.1002/j.1460-2075.1987.tb02455.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Rammensee H. G., Robinson P. J., Crisanti A., Bevan M. J. Restricted recognition of beta 2-microglobulin by cytotoxic T lymphocytes. Nature. 1986 Feb 6;319(6053):502–504. doi: 10.1038/319502a0. [DOI] [PubMed] [Google Scholar]
  22. Raulet D. H., Garman R. D., Saito H., Tonegawa S. Developmental regulation of T-cell receptor gene expression. Nature. 1985 Mar 7;314(6006):103–107. doi: 10.1038/314103a0. [DOI] [PubMed] [Google Scholar]
  23. Reichert R. A., Jerabek L., Gallatin W. M., Butcher E. C., Weissman I. L. Ontogeny of lymphocyte homing receptor expression in the mouse thymus. J Immunol. 1986 May 15;136(10):3535–3542. [PubMed] [Google Scholar]
  24. Scollay R., Bartlett P., Shortman K. T cell development in the adult murine thymus: changes in the expression of the surface antigens Ly2, L3T4 and B2A2 during development from early precursor cells to emigrants. Immunol Rev. 1984 Dec;82:79–103. doi: 10.1111/j.1600-065x.1984.tb01118.x. [DOI] [PubMed] [Google Scholar]
  25. Sideras P., Bergstedt-Lindqvist S., MacDonald H. R., Severinson E. Secretion of IgG1 induction factor by T cell clones and hybridomas. Eur J Immunol. 1985 Jun;15(6):586–593. doi: 10.1002/eji.1830150611. [DOI] [PubMed] [Google Scholar]
  26. Snodgrass H. R., Dembić Z., Steinmetz M., von Boehmer H. Expression of T-cell antigen receptor genes during fetal development in the thymus. Nature. 1985 May 16;315(6016):232–233. doi: 10.1038/315232a0. [DOI] [PubMed] [Google Scholar]
  27. Snodgrass H. R., Kisielow P., Kiefer M., Steinmetz M., von Boehmer H. Ontogeny of the T-cell antigen receptor within the thymus. Nature. 1985 Feb 14;313(6003):592–595. doi: 10.1038/313592a0. [DOI] [PubMed] [Google Scholar]
  28. Traunecker A., Kiefer M., Dembić Z., Steinmetz M., Karjalainen K. Rearrangements of T cell receptor loci can be found only rarely in B lymphoid cells. Eur J Immunol. 1986 Apr;16(4):430–434. doi: 10.1002/eji.1830160420. [DOI] [PubMed] [Google Scholar]
  29. Traunecker A., Oliveri F., Allen N., Karjalainen K. Normal T cell development is possible without 'functional' gamma chain genes. EMBO J. 1986 Jul;5(7):1589–1593. doi: 10.1002/j.1460-2075.1986.tb04400.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Weiss A., Newton M., Crommie D. Expression of T3 in association with a molecule distinct from the T-cell antigen receptor heterodimer. Proc Natl Acad Sci U S A. 1986 Sep;83(18):6998–7002. doi: 10.1073/pnas.83.18.6998. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Yancopoulos G. D., Alt F. W. Developmentally controlled and tissue-specific expression of unrearranged VH gene segments. Cell. 1985 Feb;40(2):271–281. doi: 10.1016/0092-8674(85)90141-2. [DOI] [PubMed] [Google Scholar]

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