Abstract
A synthetic peptide, (DPPPPNPN)2D, representing a subunit of the repeat domain of the Plasmodium berghei circumsporozoite protein, was conjugated to tetanus toxoid using bisdiazobenzidine. Immunization of mice and rats with the conjugate induced high serum titers of antibodies to the parasite, and most of the animals were completely protected from malaria infection when challenged with sporozoites.
Full Text
The Full Text of this article is available as a PDF (401.8 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Barr P. J., Gibson H. L., Enea V., Arnot D. E., Hollingdale M. R., Nussenzweig V. Expression in yeast of a Plasmodium vivax antigen of potential use in a human malaria vaccine. J Exp Med. 1987 Apr 1;165(4):1160–1171. doi: 10.1084/jem.165.4.1160. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dyrberg T., Oldstone M. B. Peptides as antigens. Importance of orientation. J Exp Med. 1986 Oct 1;164(4):1344–1349. doi: 10.1084/jem.164.4.1344. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Egan J. E., Weber J. L., Ballou W. R., Hollingdale M. R., Majarian W. R., Gordon D. M., Maloy W. L., Hoffman S. L., Wirtz R. A., Schneider I. Efficacy of murine malaria sporozoite vaccines: implications for human vaccine development. Science. 1987 Apr 24;236(4800):453–456. doi: 10.1126/science.3551073. [DOI] [PubMed] [Google Scholar]
- Ferreira A., Enea V., Morimoto T., Nussenzweig V. Infectivity of Plasmodium berghei sporozoites measured with a DNA probe. Mol Biochem Parasitol. 1986 May;19(2):103–109. doi: 10.1016/0166-6851(86)90114-3. [DOI] [PubMed] [Google Scholar]
- Ferreira A., Schofield L., Enea V., Schellekens H., van der Meide P., Collins W. E., Nussenzweig R. S., Nussenzweig V. Inhibition of development of exoerythrocytic forms of malaria parasites by gamma-interferon. Science. 1986 May 16;232(4752):881–884. doi: 10.1126/science.3085218. [DOI] [PubMed] [Google Scholar]
- Herrington D. A., Clyde D. F., Losonsky G., Cortesia M., Murphy J. R., Davis J., Baqar S., Felix A. M., Heimer E. P., Gillessen D. Safety and immunogenicity in man of a synthetic peptide malaria vaccine against Plasmodium falciparum sporozoites. Nature. 1987 Jul 16;328(6127):257–259. doi: 10.1038/328257a0. [DOI] [PubMed] [Google Scholar]
- Mitchell A. R., Erickson B. W., Ryabtsev M. N., Hodges R. S., Merrifield R. B. Tert-butoxycarbonylaminoacyl-4-(oxymethyl)-phenylacetamidomethyl-resin, a more acid-resistant support for solid-phase peptide synthesis. J Am Chem Soc. 1976 Nov 10;98(23):7357–7362. doi: 10.1021/ja00439a041. [DOI] [PubMed] [Google Scholar]
- Nardin E. H., Nussenzweig R. S., McGregor I. A., Bryan J. H. Antibodies to sporozoites: their frequent occurrence in individuals living in an area of hyperendemic malaria. Science. 1979 Nov 2;206(4418):597–599. doi: 10.1126/science.386511. [DOI] [PubMed] [Google Scholar]
- Nussenzweig V., Nussenzweig R. S. Development of a sporozoite malaria vaccine. Am J Trop Med Hyg. 1986 Jul;35(4):678–688. doi: 10.4269/ajtmh.1986.35.678. [DOI] [PubMed] [Google Scholar]
- Zavala F., Tam J. P., Hollingdale M. R., Cochrane A. H., Quakyi I., Nussenzweig R. S., Nussenzweig V. Rationale for development of a synthetic vaccine against Plasmodium falciparum malaria. Science. 1985 Jun 21;228(4706):1436–1440. doi: 10.1126/science.2409595. [DOI] [PubMed] [Google Scholar]