Abstract
RJ 2.2.5 is an immunoselected mutant of the Burkitt lymphoma line Raji. It fails to display MHC class II antigens at the cell surface due to a transcriptional defect. We have identified the function of a regulatory factor that is defective in RJ 2.2.5 cells; this factor is absolutely required for the activity of an MHC class II gene enhancer.
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Selected References
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- Accolla R. S., Carra G., Guardiola J. Reactivation by a trans-acting factor of human major histocompatibility complex Ia gene expression in interspecies hybrids between an Ia-negative human B-cell variant and an Ia-positive mouse B-cell lymphoma. Proc Natl Acad Sci U S A. 1985 Aug;82(15):5145–5149. doi: 10.1073/pnas.82.15.5145. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Accolla R. S. Human B cell variants immunoselected against a single Ia antigen subset have lost expression of several Ia antigen subsets. J Exp Med. 1983 Mar 1;157(3):1053–1058. doi: 10.1084/jem.157.3.1053. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Accolla R. S., Jotterand-Bellomo M., Scarpellino L., Maffei A., Carra G., Guardiola J. aIr-1, a newly found locus on mouse chromosome 16 encoding a trans-acting activator factor for MHC class II gene expression. J Exp Med. 1986 Jul 1;164(1):369–374. doi: 10.1084/jem.164.1.369. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Accolla R. S., Pierres M. Structural heterogeneity of the human Ia molecular pool as detected by cross-reacting mouse monoclonal antibodies. J Immunol. 1983 Jan;130(1):283–288. [PubMed] [Google Scholar]
- Accolla R. S., Scarpellino L., Carra G., Guardiola J. Trans-acting element(s) operating across species barriers positively regulate expression of major histocompatibility complex class II genes. J Exp Med. 1985 Oct 1;162(4):1117–1133. doi: 10.1084/jem.162.4.1117. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Atchison M. L., Perry R. P. The role of the kappa enhancer and its binding factor NF-kappa B in the developmental regulation of kappa gene transcription. Cell. 1987 Jan 16;48(1):121–128. doi: 10.1016/0092-8674(87)90362-x. [DOI] [PubMed] [Google Scholar]
- Benoist C., Chambon P. In vivo sequence requirements of the SV40 early promotor region. Nature. 1981 Mar 26;290(5804):304–310. doi: 10.1038/290304a0. [DOI] [PubMed] [Google Scholar]
- Dorn A., Bollekens J., Staub A., Benoist C., Mathis D. A multiplicity of CCAAT box-binding proteins. Cell. 1987 Sep 11;50(6):863–872. doi: 10.1016/0092-8674(87)90513-7. [DOI] [PubMed] [Google Scholar]
- Dorn A., Durand B., Marfing C., Le Meur M., Benoist C., Mathis D. Conserved major histocompatibility complex class II boxes--X and Y--are transcriptional control elements and specifically bind nuclear proteins. Proc Natl Acad Sci U S A. 1987 Sep;84(17):6249–6253. doi: 10.1073/pnas.84.17.6249. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Guardiola J., Scarpellino L., Carra G., Accolla R. S. Stable integration of mouse DNA into Ia-negative human B-lymphoma cells causes reexpression of the human Ia-positive phenotype. Proc Natl Acad Sci U S A. 1986 Oct;83(19):7415–7418. doi: 10.1073/pnas.83.19.7415. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hooft van Huijsduijnen R. A., Bollekens J., Dorn A., Benoist C., Mathis D. Properties of a CCAAT box-binding protein. Nucleic Acids Res. 1987 Sep 25;15(18):7265–7282. doi: 10.1093/nar/15.18.7265. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hume C. R., Accolla R. S., Lee J. S. Defective HLA class II expression in a regulatory mutant is partially complemented by activated ras oncogenes. Proc Natl Acad Sci U S A. 1987 Dec;84(23):8603–8607. doi: 10.1073/pnas.84.23.8603. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Landias D., Beck B. N., Buerstedde J. M., Degraw S., Klein D., Koch N., Murphy D., Pierres M., Tada T., Yamamoto K. The assignment of chain specificities for anti-Ia monoclonal antibodies using L cell transfectants. J Immunol. 1986 Nov 1;137(9):3002–3005. [PubMed] [Google Scholar]
- Levine F., Erlich H. A., Mach B., Pious D. Transcriptional regulation of HLA class II and invariant chain genes. J Immunol. 1985 Jan;134(1):637–640. [PubMed] [Google Scholar]
- Long E. O., Mach B., Accolla R. S. Ia-negative B-cell variants reveal a coordinate regulation in the transcription of the HLA class II gene family. Immunogenetics. 1984;19(4):349–353. doi: 10.1007/BF00345408. [DOI] [PubMed] [Google Scholar]
- Mathis D. J., Benoist C. O., Williams V. E., 2nd, Kanter M. R., McDevitt H. O. The murine E alpha immune response gene. Cell. 1983 Mar;32(3):745–754. doi: 10.1016/0092-8674(83)90060-0. [DOI] [PubMed] [Google Scholar]
- McCutchan J. H., Pagano J. S. Enchancement of the infectivity of simian virus 40 deoxyribonucleic acid with diethylaminoethyl-dextran. J Natl Cancer Inst. 1968 Aug;41(2):351–357. [PubMed] [Google Scholar]
- Miwa K., Doyle C., Strominger J. L. Sequence-specific interactions of nuclear factors with conserved sequences of human class II major histocompatibility complex genes. Proc Natl Acad Sci U S A. 1987 Jul;84(14):4939–4943. doi: 10.1073/pnas.84.14.4939. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Polla B. S., Poljak A., Geier S. G., Nathenson S. G., Ohara J., Paul W. E., Glimcher L. H. Three distinct signals can induce class II gene expression in a murine pre-B-cell line. Proc Natl Acad Sci U S A. 1986 Jul;83(13):4878–4882. doi: 10.1073/pnas.83.13.4878. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sullivan K. E., Peterlin B. M. Transcriptional enhancers in the HLA-DQ subregion. Mol Cell Biol. 1987 Sep;7(9):3315–3319. doi: 10.1128/mcb.7.9.3315. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Zenke M., Grundström T., Matthes H., Wintzerith M., Schatz C., Wildeman A., Chambon P. Multiple sequence motifs are involved in SV40 enhancer function. EMBO J. 1986 Feb;5(2):387–397. doi: 10.1002/j.1460-2075.1986.tb04224.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- de Préval C., Lisowska-Grospierre B., Loche M., Griscelli C., Mach B. A trans-acting class II regulatory gene unlinked to the MHC controls expression of HLA class II genes. Nature. 1985 Nov 21;318(6043):291–293. doi: 10.1038/318291a0. [DOI] [PubMed] [Google Scholar]