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. 1975 Apr 1;141(4):761–774.

Immunologic injury of cultured cells infected with measles virus. I. role of IfG antibody and the alternative complement pathway

PMCID: PMC2189741  PMID: 1092789

Abstract

In these studies, a number of human cell lines including epithelial, neural, glial, and lymphoid cells infected with several strains of measles virus were found to be lysed upon incubation with fresh sera from humans containing antibody measles virus. In all instances, the cytolytic event was mediated by alternative complement (C) pathway without a significant contribution from classical pathway. In contrast, isolated measles virus in conjunction with antibody was found to selectively activate the classical C pathway. Measles antibodies of the IgG class, but not the IgA class, possessed cytolytic potential against cells infected with measles virus. Human IgG antibodies could directly activate the alternative C pathway. No defect was found in cytolytic measles antibody in sera or cerebrospinal fluid from patients with subacute sclerosing panencephalitis, nor was the alternative C pathway impaired in sera from these patients. Sera from newborn humans exhibited a functional alternative C pathway.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adams J. M., Baird C., Filloy L. Inclusion bodies in measles encephalitis. JAMA. 1966 Jan 24;195(4):290–298. [PubMed] [Google Scholar]
  2. Brier A. M., Wohlenberg C., Rosenthal J., Mage M., Notkins A. L. Inhibition or enhancement of immunological injury of virus-infected cells. Proc Natl Acad Sci U S A. 1971 Dec;68(12):3073–3077. doi: 10.1073/pnas.68.12.3073. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Catanzaro P. J., Brandt W. E., Hogrefe W. R., Russell P. K. Detection of dengue cell-surface antigens by peroxidase-labeled antibodies and immune cytolysis. Infect Immun. 1974 Aug;10(2):381–388. doi: 10.1128/iai.10.2.381-388.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cooper N. R., Polley M. J., Oldstone M. B. Failure of terminal complement components to induce lysis of Moloney virus transformed lymphocytes. J Immunol. 1974 Feb;112(2):866–868. [PubMed] [Google Scholar]
  5. Cooper N. R., ten Bensel R., Kohler P. F. Studies of an additional kindred with hereditary deficiency of the second component of human complement (C2) and description of a new method for the quantitation of C2. J Immunol. 1968 Dec;101(6):1176–1182. [PubMed] [Google Scholar]
  6. Ferrone S., Cooper N. R., Pellegrino M. A., Reisfeld R. A. Activation of human complement by human lymphoid cells sensitized with histocompatibility alloantisera. Proc Natl Acad Sci U S A. 1973 Dec;70(12):3665–3668. doi: 10.1073/pnas.70.12.3665. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Ferrone S., Pellegrino M. A., Reisfeld R. A. A rapid method for direct HL-A typing of cultured lymphoid cells. J Immunol. 1971 Aug;107(2):613–615. [PubMed] [Google Scholar]
  8. Holmes K. V., Klenk H. D., Choppin P. W. A comparison of immune cytolysis and virus-induced fusion of sensitive and resistant cell types. Proc Soc Exp Biol Med. 1969 Jun;131(2):651–657. doi: 10.3181/00379727-131-33945. [DOI] [PubMed] [Google Scholar]
  9. Ishizaka T., Soto C. S., Ishizaka K. Characteristics of complement fixation by aggregated IgE. J Immunol. 1972 Dec;109(6):1290–1295. [PubMed] [Google Scholar]
  10. Joseph B. S., Oldstone M. B. Antibody-induced redistribution of measles virus antigens on the cell surface. J Immunol. 1974 Oct;113(4):1205–1209. [PubMed] [Google Scholar]
  11. KOPROWSKI H. The role of hyperergy in measles encephalitis. Am J Dis Child. 1962 Mar;103:273–278. doi: 10.1001/archpedi.1962.02080020285019. [DOI] [PubMed] [Google Scholar]
  12. Lerner R. A., Oldstone M. B., Cooper N. R. Cell cycle-dependent immune lysis of Moloney virus-transformed lymphocytes: presence of viral antigen, accessibility to antibody, and complement activation. Proc Natl Acad Sci U S A. 1971 Oct;68(10):2584–2588. doi: 10.1073/pnas.68.10.2584. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Mannweiler K. Cytologic ultrastructural considerations and studies of the pathogenesis of demyelinating processes. Int Arch Allergy Appl Immunol. 1969;36(Suppl):41–58. [PubMed] [Google Scholar]
  14. McConahey P. J., Dixon F. J. A method of trace iodination of proteins for immunologic studies. Int Arch Allergy Appl Immunol. 1966;29(2):185–189. doi: 10.1159/000229699. [DOI] [PubMed] [Google Scholar]
  15. Minagawa T., Yamada M. Studies on the persistent infection with measles virus in HeLa cells. 3. Immunolysis of cells in carrier state by anti-measles sera. Jpn J Microbiol. 1971 Jul;15(4):341–350. doi: 10.1111/j.1348-0421.1971.tb00590.x. [DOI] [PubMed] [Google Scholar]
  16. Müller-Eberhard H. J., Fjellström K. E. Isolation of the anticomplementary protein from cobra venom and its mode of action on C3. J Immunol. 1971 Dec;107(6):1666–1672. [PubMed] [Google Scholar]
  17. OLD L., BOYSE E. A., LILLY F. FORMATION OF CYTOXIC ANTIBODY AGAINST LEUKEMIAS INDUCED BY FRIEND VIRUS. Cancer Res. 1963 Aug;23:1063–1068. [PubMed] [Google Scholar]
  18. Oldstone M. B., Dixon F. J. Acute viral infection: tissue injury mediated by anti-viral antibody through a complement effector system. J Immunol. 1971 Nov;107(5):1274–1280. [PubMed] [Google Scholar]
  19. Oldstone M. B., Dixon F. J. Direct immunofluorescent tissue culture assay for lymphocytic choriomeningitis virus. J Immunol. 1968 May;100(5):1135–1138. [PubMed] [Google Scholar]
  20. Oldstone M. B., Tishon A., Chiller J. M., Weigle W. O., Dixon F. J. Effect of chronic viral infection on the immune system. I. Comparison of the immune responsiveness of mice chronically infected with LCM virus with that of noninfected mice. J Immunol. 1973 May;110(5):1268–1278. [PubMed] [Google Scholar]
  21. Pellegrino M. A., Ferrone S., Cooper N. R., Dierich M. P., Reisfeld R. A. Variation in susceptibility of a human lymphoid cell line to immune lysis during the cell cycle. Lack of correlation with antigen density and complement binding. J Exp Med. 1974 Aug 1;140(2):578–590. doi: 10.1084/jem.140.2.578. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. SLETTENMARK B., KLEIN E. Cytotoxic and neutralization tests with serum and lymph node cells of isologous mice with induced resistance against gross lymphomas. Cancer Res. 1962 Sep;22:947–954. [PubMed] [Google Scholar]
  23. Sandberg A. L., Snyderman R., Frank M. M., Osler A. G. Production of chemotactic activity by guinea pig immunoglobulins following activation of the C3 complement shunt pathway. J Immunol. 1972 May;108(5):1227–1231. [PubMed] [Google Scholar]
  24. Vallota E. H., Götze O., Spiegelberg H. L., Forristal J., West C. D., Müller-Eberhard H. J. A serum factor in chronic hypocomplementemic hephritis distinct from immunoglobulins and activating the alternate pathway of complement. J Exp Med. 1974 May 1;139(5):1249–1261. doi: 10.1084/jem.139.5.1249. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Wiktor T. J., Kuwert E., Koprowski H. Immune lysis of rabies virus-infected cells. J Immunol. 1968 Dec;101(6):1271–1282. [PubMed] [Google Scholar]

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