Abstract
Two Fc receptor-dependent tests were investigated to study the question of a relationship between Fc receptors and known cell surface antigens, in particular I region-associated (Ia) antigens: (a) a rosette assay with antibody-coated erythrocytes (EA) as indicator cells and normal mouse lymphoid cells as source of rosette-forming cells, and (b) a cytotoxicity test with antibody-coated erythrocytes as target cells and normal mouse spleen cells as a source of cytotoxic cells (K cells). EA rosettes were specifically inhibited by antibodies reacting with Ia antigens. Various other antisera reacting with antigens on B lymphocytes, like anti-Ly 4.2 (raised in H-2 identical mice), rabbit antimouse B-cell serum, or rabbit antimouse immunoglobulin, also specifically inhibited the rosettes. No inhibition occurred in the presence of allogeneic or xenogeneic antisera reacting with T lymphocytes. K-cell cytotoxicity was specifically inhibited by each of the antisera (reacting with either B cells or T cells). F(ab')2 fragments of anti-Ia antibodies could still specifically inhibit EA rosettes but they could not inhibit K-cell cytotoxicity. Similar results were obtained with F(ab')2 fragments of anti-immunoglobulin antibodies. These results indicate that the mechanism of inhibition of Fc receptors in the two tests was different. In neither of the tests could we find any evidence for a unique association between the Fc receptors and Ia antigens. The Fc receptors on K cells did not seem to be associated at all with Ia antigens.
Full Text
The Full Text of this article is available as a PDF (503.4 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Cline M. J., Sprent J., Warner N. L., Harris A. W. Receptors for immunoglobulin on B lymphocytes and cells of a cultured plasma cell tumor. J Immunol. 1972 Apr;108(4):1126–1128. [PubMed] [Google Scholar]
- David C. S., Shreffler D. C., Frelinger J. A. New lymphocyte antigen system (Lna) controlled by the Ir region of the mouse H-2 complex. Proc Natl Acad Sci U S A. 1973 Sep;70(9):2509–2514. doi: 10.1073/pnas.70.9.2509. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dickler H. B., Sachs D. H. Evidence for identity or close association of the Fc receptor of B lymphocytes and alloantigens determined by the Ir region of the H-2 complex. J Exp Med. 1974 Sep 1;140(3):779–796. doi: 10.1084/jem.140.3.779. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Halloran P., Schirrmacher V., Festenstein H. A new sensitive assay for antibody against cell surface antigens based on inhibition of cell-dependent antibody-mediated cytotoxicity. I. Specificity and sensitivity. J Exp Med. 1974 Nov 1;140(5):1348–1363. doi: 10.1084/jem.140.5.1348. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kerbel R. S., Davies A. J. The possible biological significance of Fc receptors on mammalian lymphocytes and tumor cells. Cell. 1974 Oct;3(2):105–112. doi: 10.1016/0092-8674(74)90113-5. [DOI] [PubMed] [Google Scholar]
- McKenzie I. F., Plate J. M. Ly-4.2: a cell membrane alloantigen of murine B lymphocytes. III. In vitro studies. Cell Immunol. 1974 Dec;14(3):376–385. doi: 10.1016/0008-8749(74)90187-7. [DOI] [PubMed] [Google Scholar]
- Parish C. R., Hayward J. A. The lymphocyte surface. I. Relation between Fc receptors, C'3 receptors and surface immunoglobulin. Proc R Soc Lond B Biol Sci. 1974 Aug 27;187(1086):47–63. doi: 10.1098/rspb.1974.0060. [DOI] [PubMed] [Google Scholar]
- Raff M. C. Surface antigenic markers for distinguishing T and B lymphocytes in mice. Transplant Rev. 1971;6:52–80. doi: 10.1111/j.1600-065x.1971.tb00459.x. [DOI] [PubMed] [Google Scholar]
- Rubin B. The immune response against hapten-autologous protein conjugates in the mouse. I. Specificity of antibodies produced during the primary response against dinitrophenylated mouse serum albumin. Eur J Immunol. 1973 Jan;3(1):26–32. doi: 10.1002/eji.1830030107. [DOI] [PubMed] [Google Scholar]