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. 1985 Apr;162(1):235–241. doi: 10.1128/jb.162.1.235-241.1985

Conjugative plasmids of enteric bacteria from many different incompatibility groups have similar genes for single-stranded DNA-binding proteins.

E I Golub, K B Low
PMCID: PMC218979  PMID: 3884590

Abstract

Among 30 conjugative plasmids of enteric bacteria from 23 incompatibility (Inc) groups, we found 19 (from 12 Inc groups) which can complement defects caused by a defective single-stranded DNA-binding protein of Escherichia coli K-12. The genes which are responsible for the complementation from three of these plasmids (Inc groups I1, Y, and 9) were cloned. These genes showed extensive homology with each other and with the E. coli F factor ssb gene (formerly denoted ssf), which codes for a single-stranded DNA binding protein. The proteins coded for by the cloned genes bound tightly to single-stranded DNA. Six other ssb- -complementing plasmids were tested for homology to the F factor ssb gene, and all of these showed homology, as did one of the ssb- -noncomplementing plasmids. Plasmids from a total of 13 different Inc groups of enteric bacteria were found to be likely to have genes with some homology to the ssb gene of the F factor. For plasmids from several different Inc groups, we found no evidence for strong homology with ssb of the F factor.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bolivar F., Rodriguez R. L., Greene P. J., Betlach M. C., Heyneker H. L., Boyer H. W., Crosa J. H., Falkow S. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system. Gene. 1977;2(2):95–113. [PubMed] [Google Scholar]
  2. Chase J. W., Merrill B. M., Williams K. R. F sex factor encodes a single-stranded DNA binding protein (SSB) with extensive sequence homology to Escherichia coli SSB. Proc Natl Acad Sci U S A. 1983 Sep;80(18):5480–5484. doi: 10.1073/pnas.80.18.5480. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Glassberg J., Meyer R. R., Kornberg A. Mutant single-strand binding protein of Escherichia coli: genetic and physiological characterization. J Bacteriol. 1979 Oct;140(1):14–19. doi: 10.1128/jb.140.1.14-19.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Golub E. I., Low K. B. Indirect stimulation of genetic recombination. Proc Natl Acad Sci U S A. 1983 Mar;80(5):1401–1405. doi: 10.1073/pnas.80.5.1401. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Grindley N. D., Humphreys G. O., Anderson E. S. Molecular studies of R factor compatibility groups. J Bacteriol. 1973 Jul;115(1):387–398. doi: 10.1128/jb.115.1.387-398.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Guyer M. S., Reed R. R., Steitz J. A., Low K. B. Identification of a sex-factor-affinity site in E. coli as gamma delta. Cold Spring Harb Symp Quant Biol. 1981;45(Pt 1):135–140. doi: 10.1101/sqb.1981.045.01.022. [DOI] [PubMed] [Google Scholar]
  7. Johnson B. F. Suppression of the lexC (ssbA) mutation of Escherichia coli by a mutant of bacteriophage P1. Mol Gen Genet. 1982;186(1):122–126. doi: 10.1007/BF00422923. [DOI] [PubMed] [Google Scholar]
  8. Kolodkin A. L., Capage M. A., Golub E. I., Low K. B. F sex factor of Escherichia coli K-12 codes for a single-stranded DNA binding protein. Proc Natl Acad Sci U S A. 1983 Jul;80(14):4422–4426. doi: 10.1073/pnas.80.14.4422. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Lloyd R. G., Barbour S. D. The genetic location of the sbcA gene of Escherichia coli. Mol Gen Genet. 1974;134(2):157–171. doi: 10.1007/BF00268417. [DOI] [PubMed] [Google Scholar]
  10. Roussel A. F., Chabbert Y. A. Taxonomy and epidemiology of gram-negative bacterial plasmids studied by DNA-DNA filter hybridization in formamide. J Gen Microbiol. 1978 Feb;104(2):269–276. doi: 10.1099/00221287-104-2-269. [DOI] [PubMed] [Google Scholar]
  11. Sancar A., Hack A. M., Rupp W. D. Simple method for identification of plasmid-coded proteins. J Bacteriol. 1979 Jan;137(1):692–693. doi: 10.1128/jb.137.1.692-693.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Sancar A., Williams K. R., Chase J. W., Rupp W. D. Sequences of the ssb gene and protein. Proc Natl Acad Sci U S A. 1981 Jul;78(7):4274–4278. doi: 10.1073/pnas.78.7.4274. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Sharp P. A., Cohen S. N., Davidson N. Electron microscope heteroduplex studies of sequence relations among plasmids of Escherichia coli. II. Structure of drug resistance (R) factors and F factors. J Mol Biol. 1973 Apr 5;75(2):235–255. doi: 10.1016/0022-2836(73)90018-1. [DOI] [PubMed] [Google Scholar]
  14. Shaw K. J., Berg C. M. Escherichia coli K-12 auxotrophs induced by insertion of the transposable element Tn5. Genetics. 1979 Jul;92(3):741–747. doi: 10.1093/genetics/92.3.741. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Soberon X., Covarrubias L., Bolivar F. Construction and characterization of new cloning vehicles. IV. Deletion derivatives of pBR322 and pBR325. Gene. 1980 May;9(3-4):287–305. doi: 10.1016/0378-1119(90)90328-o. [DOI] [PubMed] [Google Scholar]

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