Skip to main content
NCBI home page
The Journal of Experimental Medicine logoLink to The Journal of Experimental Medicine
. 1975 May 1;141(5):1101–1113. doi: 10.1084/jem.141.5.1101

Subcellular platelet factor VIII antigen and von Willebrand factor

PMCID: PMC2189790  PMID: 1168691

Abstract

Subcellular membrane and granule fractions derived from human platelets contain factor VIIII antigen and von Willebrand factor activity but not factor VII procoagulant activity. Circulating platelets constitute a significant reservoir of plasma factor VIII antigen, containing approximately 15% of the amount of factor VIII antigen present in platelet-poor plasma. The antibiotic ristocetin, which aggregates human platelets in the presence of von Willebrand factor, nonspecifically precipitates platelet membrane factor VIII antigen. Thus normal platelets contain surface-bound as well as internally stored von Willebrand factor, a protein synthesized by endothelial cells which is necessary for normal platelet function in vivo.

Full Text

The Full Text of this article is available as a PDF (786.7 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BRECKENRIDGE R. T., RATNOFF C. D. Studies on the nature of the circulating anticoagulant directed against antihemophilic factor: with notes on an assay for anthemophilic factor. Blood. 1962 Aug;20:137–149. [PubMed] [Google Scholar]
  2. Beckman B., Flores J., Witkum P. A., Sharp G. W. Studies on the mode of action of cholera toxin. Effects on solubilized adenylate cyclase. J Clin Invest. 1974 Apr;53(4):1202–1205. doi: 10.1172/JCI107660. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cooper H. A., Wagner R. H., Mosesson M. W. The cold-insoluble globulin of plasma and its relationship to factor VIII. J Lab Clin Med. 1974 Aug;84(2):258–263. [PubMed] [Google Scholar]
  4. Cuatrecasas P. Protein purification by affinity chromatography. Derivatizations of agarose and polyacrylamide beads. J Biol Chem. 1970 Jun;245(12):3059–3065. [PubMed] [Google Scholar]
  5. Gore I., Takada M., Austin J. Ultrastructural basis of experimental thrombocytopenic purpura. Arch Pathol. 1970 Sep;90(3):197–205. [PubMed] [Google Scholar]
  6. Holmberg L., Mannucci P. M., Turesson I., Ruggeri Z. M., Nilsson I. M. Factor VIII antigen in the vessel walls in von Willebrand's disease and haemophilia A. Scand J Haematol. 1974;13(1):33–38. doi: 10.1111/j.1600-0609.1974.tb00232.x. [DOI] [PubMed] [Google Scholar]
  7. Howard M. A., Firkin B. G. Ristocetin--a new tool in the investigation of platelet aggregation. Thromb Diath Haemorrh. 1971 Oct 31;26(2):362–369. [PubMed] [Google Scholar]
  8. Howard M. A., Montgomery D. C., Hardisty R. M. Factor-VIII-related antigen in platelets. Thromb Res. 1974 May;4(5):617–624. doi: 10.1016/0049-3848(74)90218-7. [DOI] [PubMed] [Google Scholar]
  9. Hoyer L. W. Immunologic studies of antihemophilic factor (AHF, factor VIII). IV. Radioimmunoassay of AHF antigen. J Lab Clin Med. 1972 Dec;80(6):822–833. [PubMed] [Google Scholar]
  10. Jaffe E. A., Hoyer L. W., Nachman R. L. Synthesis of antihemophilic factor antigen by cultured human endothelial cells. J Clin Invest. 1973 Nov;52(11):2757–2764. doi: 10.1172/JCI107471. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Jaffe E. A., Hoyer L. W., Nachman R. L. Synthesis of von Willebrand factor by cultured human endothelial cells. Proc Natl Acad Sci U S A. 1974 May;71(5):1906–1909. doi: 10.1073/pnas.71.5.1906. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kolb W. P., Haxby J. A., Arroyave C. M., Müller-Eberhard H. J. Molecular analysis of the membrane attack mechanism of complement. J Exp Med. 1972 Mar 1;135(3):549–566. doi: 10.1084/jem.135.3.549. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Legaz M. E., Schmer G., Counts R. B., Davie E. W. Isolation and characterization of human Factor VIII (antihemophilic factor). J Biol Chem. 1973 Jun 10;248(11):3946–3955. [PubMed] [Google Scholar]
  14. Levy-Toledano S., Caen J. P., Halmos T., Mester L. Dissociation between human platelet agglomerating activity and factor VIII procoagulant activity of bovine plasma preparations by chemical treatment. I. Effect of neuraminidase. Pathol Biol (Paris) 1973 Nov;21(Suppl):60–62. [PubMed] [Google Scholar]
  15. Marcus A. J., Zucker-Franklin D., Safier L. B., Ullman H. L. Studies on human platelet granules and membranes. J Clin Invest. 1966 Jan;45(1):14–28. doi: 10.1172/JCI105318. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Nachman R. L., Ferris B. Studies on human platelet protease activity. J Clin Invest. 1969 Nov;47(11):2530–2540. doi: 10.1172/JCI105935. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Nachman R. L., Hubbard A., Ferris B. Iodination of the human platelet membrane. Studies of the major surface glycoprotein. J Biol Chem. 1973 Apr 25;248(8):2928–2936. [PubMed] [Google Scholar]
  18. Nachman R. L., Marcus A. J., Safier L. B. Platelet thrombosthenin: subcellular localization and function. J Clin Invest. 1967 Aug;46(8):1380–1389. doi: 10.1172/JCI105630. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Nachman R. L., Marcus A. J., Zucker-Franklin D. Immunologic studies of proteins associated with subcellular fractions of normal human platelets. J Lab Clin Med. 1967 Apr;69(4):651–658. [PubMed] [Google Scholar]
  20. Tschopp T. B., Weiss H. J., Baumgartner H. R. Decreased adhesion of platelets to subendothelium in von Willebrand's disease. J Lab Clin Med. 1974 Feb;83(2):296–300. [PubMed] [Google Scholar]
  21. Vermylen J., Donati M. B., De Gaetano G., Verstraete M. Aggregation of human platelets by bovine or human factor VIII: role of carbohydrate side chains. Nature. 1973 Jul 20;244(5412):167–168. doi: 10.1038/244167a0. [DOI] [PubMed] [Google Scholar]
  22. Weber K., Osborn M. The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis. J Biol Chem. 1969 Aug 25;244(16):4406–4412. [PubMed] [Google Scholar]
  23. Weiss H. J., Hoyer L. W., Rickles F. R., Varma A., Rogers J. Quantitative assay of a plasma factor deficient in von Willebrand's disease that is necessary for platelet aggregation. Relationship to factor VIII procoagulant activity and antigen content. J Clin Invest. 1973 Nov;52(11):2708–2716. doi: 10.1172/JCI107465. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Weiss H. J., Rogers J., Brand H. Defective ristocetin-induced platelet aggregation in von Willebrand's disease and its correction by factor VIII. J Clin Invest. 1973 Nov;52(11):2697–2707. doi: 10.1172/JCI107464. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Weiss H. J. Von Willebrand's disease--diagnostic criteria. Blood. 1968 Oct;32(4):668–679. [PubMed] [Google Scholar]
  26. Zimmerman T. S., Ratnoff O. D., Powell A. E. Immunologic differentiation of classic hemophilia (factor 8 deficiency) and von Willebrand's dissase, with observations on combined deficiencies of antihemophilic factor and proaccelerin (factor V) and on an acquired circulating anticoagulant against antihemophilic factor. J Clin Invest. 1971 Jan;50(1):244–254. doi: 10.1172/JCI106480. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from The Journal of Experimental Medicine are provided here courtesy of The Rockefeller University Press

RESOURCES