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. 1975 May 1;141(5):1047–1056. doi: 10.1084/jem.141.5.1047

Induction of resistance to antibody-mediated cytotoxicity. H-2, Ia, and Ig antigens are independent entities in the membrane of mouse lymphocytes

PMCID: PMC2189792  PMID: 47892

Abstract

Mouse spleen or thymus lymphocytes incubated with monospecific H-2 or Ia alloantisera and then coated with a xenogeneic antimouse Ig serum become specifically resistant to the alloantiserum (and complement) they have been incubated with. This so called "lysostrip method" was used to investigate the molecular interrelationships of antigens in the mouse lymphocyte membrane. The results of this investigation confirm that H-2K and H-2D antigens are carried by two distinct populations of molecules. They provide evidence that the Ia antigens move in the membrane independently of both H2-K and H-2D antigens; and finally they demonstrate absence of any physical linkage between Ig receptors in B cells, on the one hand, and Ia, H-2K, and H-2D molecules on the other hand.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Amos D. B., Bashir H., Boyle W., MacQueen M., Tiilikainen A. A simple micro cytotoxicity test. Transplantation. 1969 Mar;7(3):220–223. doi: 10.1097/00007890-196903000-00023. [DOI] [PubMed] [Google Scholar]
  2. Cullen S. E., Bernoco D., Carbonara A. O., Jacot-Guillarmod H., Trinchieri G., Ceppellini R. Fate of HL-A antigens and antibodies at the lymphocyte surface. Transplant Proc. 1973 Dec;5(4):1835–1847. [PubMed] [Google Scholar]
  3. Cullen S. E., David C. S., Shreffler D. C., Nathenson S. G. Membrane molecules determined by the H-2 associated immune response region: isolation and some properties. Proc Natl Acad Sci U S A. 1974 Mar;71(3):648–652. doi: 10.1073/pnas.71.3.648. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cullen S. E., Schwartz B. D., Nathenson S. G., Cherry M. The molecular basis of codominant expression of the histocompatibility-2 genetic region. Proc Natl Acad Sci U S A. 1972 Jun;69(6):1394–1397. doi: 10.1073/pnas.69.6.1394. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. David C. S., Shreffler D. C., Frelinger J. A. New lymphocyte antigen system (Lna) controlled by the Ir region of the mouse H-2 complex. Proc Natl Acad Sci U S A. 1973 Sep;70(9):2509–2514. doi: 10.1073/pnas.70.9.2509. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hauptfeld V., Hauptfeld M., Klein J. Tissue distribution of I region-associated antigens in the mouse. J Immunol. 1974 Jul;113(1):181–188. [PubMed] [Google Scholar]
  7. Hauptfeld V., Klein D., Klein J. Serological identification of an Ir-region product. Science. 1973 Jul 13;181(4095):167–169. doi: 10.1126/science.181.4095.167. [DOI] [PubMed] [Google Scholar]
  8. Klein J., Klein D., Shreffler D. C. H-2 types of translocation stocks T(2;9)138Ca, T(9;13)-190Ca and an H-2 recombinant. Transplantation. 1970 Oct;10(4):309–320. doi: 10.1097/00007890-197010000-00006. [DOI] [PubMed] [Google Scholar]
  9. Klein J., Shreffler D. C. The H-2 model for the major histocompatibility systems. Transplant Rev. 1971;6:3–29. doi: 10.1111/j.1600-065x.1971.tb00457.x. [DOI] [PubMed] [Google Scholar]
  10. Neauport-Sautes C., Lilly F., Silvestre D., Kourilsky F. M. Independence of H-2K and H-2D antigenic determinants on the surface of mouse lymphocytes. J Exp Med. 1973 Feb 1;137(2):511–526. doi: 10.1084/jem.137.2.511. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. REIF A. E., ALLEN J. M. THE AKR THYMIC ANTIGEN AND ITS DISTRIBUTION IN LEUKEMIAS AND NERVOUS TISSUES. J Exp Med. 1964 Sep 1;120:413–433. doi: 10.1084/jem.120.3.413. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Sachs D. H., Cone J. L. A mouse B-cell alloantigen determined by gene(s) linked to the major histocompatibility complex. J Exp Med. 1973 Dec 1;138(6):1289–1304. doi: 10.1084/jem.138.6.1289. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Singer S. J., Nicolson G. L. The fluid mosaic model of the structure of cell membranes. Science. 1972 Feb 18;175(4023):720–731. doi: 10.1126/science.175.4023.720. [DOI] [PubMed] [Google Scholar]
  14. Snell G. D., Cherry M., Démant P. Evidence that H-2 private specificities can be arranged in two mutually exclusive systems possibly homologous with two subsystems of HL-A. Transplant Proc. 1971 Mar;3(1):183–186. [PubMed] [Google Scholar]
  15. Stackpole C. W., Jacobson J. B., Lardis M. P. Antigenic modulation in vitro. I. Fate of thymus-leukemia (TL) antigen-antibody complexes following modulation of TL antigenicity from the surfaces of mouse leukemia cells and thymocytes. J Exp Med. 1974 Oct 1;140(4):939–953. doi: 10.1084/jem.140.4.939. [DOI] [PMC free article] [PubMed] [Google Scholar]

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