Abstract
Rat lymphocytes in mixed cultures can reutilize tritiated thymidine from labeled granulocytes. Shortly after thymidine injections in vivo, major effects on the frequency of labeled lymphocyte mitoses in peripheral blood cultures are introduced by 10-20% polymorph contamination, even though transfer of label via supernates is not demonstrable. Cold thymidine in the cultures prevents reutilization, and has permitted reevaluation of several previous conclusions concerning the life history of lymphocytes reactive to major histocompatibility alloantigens (HARC). Rather than being predominantly recently divided cells, HARC do not appear to have an age distribution, in blood or lymph, significantly different from the general recirculating lymphocyte population. However, the ability of immunization across strong allogeneic differences to increase markedly the proportion of young HARC among the specifically responsive population has been confirmed.
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Selected References
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- Davies A. J. The thymus and the cellular basis of immunity. Transplant Rev. 1969;1:43–91. doi: 10.1111/j.1600-065x.1969.tb00136.x. [DOI] [PubMed] [Google Scholar]
- Everett N. B., Tyler R. W. Lymphopoiesis in the thymus and other tissues: functional implications. Int Rev Cytol. 1967;22:205–237. doi: 10.1016/s0074-7696(08)61836-7. [DOI] [PubMed] [Google Scholar]
- McGregor D. D., Koster F. T., Mackaness G. B. The mediator of cellular immunity. I. The life-span and circulation dynamics of the immunologically committed lymphocyte. J Exp Med. 1971 Feb 1;133(2):389–399. doi: 10.1084/jem.133.2.389. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nowell P. C., Wilson D. B. Studies on the life history of lymphocytes. I. The life-span of cells responsive in the mixed lymphocyte interaction. J Exp Med. 1971 May 1;133(5):1131–1148. doi: 10.1084/jem.133.5.1131. [DOI] [PMC free article] [PubMed] [Google Scholar]
- RIEKE W. O. The in vivo reutilization of lymphocytic and sarcoma DNA by cells growing in the peritoneal cavity. J Cell Biol. 1962 May;13:205–216. doi: 10.1083/jcb.13.2.205. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wilson D. B., Blyth JL NOWELL P. C. Quantitative studies on the mixed lymphocyte interaction in rats. 3. Kinetics of the response. J Exp Med. 1968 Nov 1;128(5):1157–1181. doi: 10.1084/jem.128.5.1157. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wilson D. B., Howard J. C., Nowell P. C. Some biological aspects of lymphocytes reactive to strong histocompatibility alloantigens. Transplant Rev. 1972;12:3–29. doi: 10.1111/j.1600-065x.1972.tb00051.x. [DOI] [PubMed] [Google Scholar]
- Wilson D. B., Nowell P. C. Quantitative studies on the mixed lymphocyte interaction in rats. V. Tempo and specificity of the proliferative response and the number of reactive cells from immunized donors. J Exp Med. 1971 Mar 1;133(3):442–453. doi: 10.1084/jem.133.3.442. [DOI] [PMC free article] [PubMed] [Google Scholar]