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. 1975 Sep 1;142(3):664–672. doi: 10.1084/jem.142.3.664

Studies on the murine Ss protein. I. Purification, molecular weight, and subunit structure

PMCID: PMC2189919  PMID: 809530

Abstract

The murine Ss protein has been isolated and purified. Using specific antisera, the radiolabeled protein has a mol wt of 120,000 in sodium dodecyl sulfate polyacrylamide gels. It is composed of two basic subunits of 23,000 and 14,000 daltons. The smaller molecular weight subunit contains a single disulfide bridge, is devoid of carbohydrate, and may represent the murine equivalent of beta2-microglobulin.

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Selected References

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  1. Cullen S. E., David C. S., Shreffler D. C., Nathenson S. G. Membrane molecules determined by the H-2 associated immune response region: isolation and some properties. Proc Natl Acad Sci U S A. 1974 Mar;71(3):648–652. doi: 10.1073/pnas.71.3.648. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Cunningham B. A., Wang J. L., Berggård I., Peterson P. A. The complete amino acid sequence of beta 2-microglobulin. Biochemistry. 1973 Nov 20;12(24):4811–4822. doi: 10.1021/bi00748a001. [DOI] [PubMed] [Google Scholar]
  3. Démant P., Capková J., Hinzová E., Vorácová B. The role of the histocompatibility-2-linked Ss-Slp region in the control of mouse complement. Proc Natl Acad Sci U S A. 1973 Mar;70(3):863–864. doi: 10.1073/pnas.70.3.863. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Ferreira A., Nussenzweig V. Genetic linkage between serum levels of the third component of complement and the H-2 complex. J Exp Med. 1975 Feb 1;141(2):513–517. doi: 10.1084/jem.141.2.513. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Fu S. M., Kunkel H. G., Brusman H. P., Allen F. H., Jr, Fotino M. Evidence for linkage between HL-A histocompatibility genes and those involved in the synthesis of the second component of complement. J Exp Med. 1974 Oct 1;140(4):1108–1111. doi: 10.1084/jem.140.4.1108. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hansen T. H., Krasteff T. N., Shreffler D. C. Quantitative variations in the expression of the mouse serum antigen Ss and its sex-limited allotype Slp. Biochem Genet. 1974 Oct;12(4):281–293. doi: 10.1007/BF00485949. [DOI] [PubMed] [Google Scholar]
  7. Marchalonis J. J. An enzymic method for the trace iodination of immunoglobulins and other proteins. Biochem J. 1969 Jun;113(2):299–305. doi: 10.1042/bj1130299. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Melcher U., Vitetta E. S., McWilliams M., Lamm M. E., Phillips-Quagliata J. M., Uhr J. W. Cell surface immunoglobulin. X. Identification of an IgD-like molecule on the surface of murine splenocytes. J Exp Med. 1974 Nov 1;140(5):1427–1431. doi: 10.1084/jem.140.5.1427. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Natori T., Katagiri M., Tanigaki N., Pressman D. The 11,000-dalton component of mouse H-2. Isolation and identification. Transplantation. 1974 Dec;18(6):550–555. doi: 10.1097/00007890-197412000-00015. [DOI] [PubMed] [Google Scholar]
  10. Ostberg L., Rask L., Wigzell H., Peterson P. A. Thymus leukaemia antigen contains beta2-microglobulin. Nature. 1975 Feb 27;253(5494):735–737. doi: 10.1038/253735a0. [DOI] [PubMed] [Google Scholar]
  11. Polley M. J., Müller-Eberhard H. J. The second component of human complement: its isolation, fragmentation by C'1 esterase, and incorporation into C'3 convertase. J Exp Med. 1968 Sep 1;128(3):533–551. doi: 10.1084/jem.128.3.533. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Rask L., Lindblom J. B., Peterson P. A. Subunit structure of H-2 alloantigens. Nature. 1974 Jun 28;249(460):833–834. doi: 10.1038/249833a0. [DOI] [PubMed] [Google Scholar]
  13. Shreffler D C, Owen R D. A Serologically Detected Variant in Mouse Serum: Inheritance and Association with the Histocompatibility-2 Locus. Genetics. 1963 Jan;48(1):9–25. doi: 10.1093/genetics/48.1.9. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Shreffler D. C. The Ss system of the mouse--a quantitative serum protein difference genetically controlled by the H-2 region. Wistar Inst Symp Monogr. 1965 Aug;3:11–19. [PubMed] [Google Scholar]
  15. Silver J., Hood L. Detergent-solubilised H-2 alloantigen is associated with a small molecular weight polypeptide. Nature. 1974 Jun 21;249(459):764–765. doi: 10.1038/249764a0. [DOI] [PubMed] [Google Scholar]
  16. Smithies O., Poulik M. D. Initiation of protein synthesis at an unusual position in an immunoglobulin gene? Science. 1972 Jan 14;175(4018):187–189. doi: 10.1126/science.175.4018.187. [DOI] [PubMed] [Google Scholar]
  17. Vitetta E. S., Baur S., Uhr J. W. Cell surface immunoglobulin. II. Isolation and characterization of immunoglobulin from mouse splenic lymphocytes. J Exp Med. 1971 Jul 1;134(1):242–264. doi: 10.1084/jem.134.1.242. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Vitetta E. S., Uhr J. W., Boyse E. A. Association of a beta2-microglobulin-like subunit with H-2 and TL alloantigens on murine thymocytes. J Immunol. 1975 Jan;114(1 Pt 1):252–254. [PubMed] [Google Scholar]

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