Skip to main content
PMC home page
The Journal of Experimental Medicine logoLink to The Journal of Experimental Medicine
. 1975 Dec 1;142(6):1477–1487. doi: 10.1084/jem.142.6.1477

Induction of cytotoxic T lymphocytes against I-region-coded determinants: in vitro evidence for a third histocompatibility locus in the mouse

PMCID: PMC2190067  PMID: 53265

Abstract

Determinants controlled by the I region of the murine H-2 complex provoked the generation of cytotoxic T lymphocytes (CTL) in both a secondary and primary mixed lymphocyte culture. The stimulating determinants appeared to be controlled by loci within the I-A subregion. The target antigens of the CTL generated were present on both lipopolysaccharide- and concanavalin-induced blast lymphocytes, but were barely detectable on phytohemagglutinin-induced blast cells. The stimulating capacity for CTL induction of a complete H-2 complex incompatibility by far exceeded the sum of H-2D/K-region and I-region incompatibility, respectively.

Full Text

The Full Text of this article is available as a PDF (675.0 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abbasi K., Festenstein H. Antigenic strength investigated by cell-mediated lympholysis in mice. Eur J Immunol. 1973 Jul;3(7):430–435. doi: 10.1002/eji.1830030711. [DOI] [PubMed] [Google Scholar]
  2. Alter B. J., Bach F. H. Role of H-2 lymphocyte-defined and serologically-defined components in the generation of cytotoxic lymphocytes. J Exp Med. 1974 Nov 1;140(5):1410–1415. doi: 10.1084/jem.140.5.1410. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Alter B. J., Schendel D. J., Bach M. L., Bach F. H., Klein J., Stimpfling J. H. Cell-mediated lympholysis. Importance of serologically defined H-2 regions. J Exp Med. 1973 May 1;137(5):1303–1309. doi: 10.1084/jem.137.5.1303. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bach F. H., Widmer M. B., Bach M. L., Klein J. Serologically defined and lymphocyte-defined components of the major histocompatibility complex in the mouse. J Exp Med. 1972 Dec 1;136(6):1430–1444. doi: 10.1084/jem.136.6.1430. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cantor H., Boyse E. A. Functional subclasses of T lymphocytes bearing different Ly antigens. II. Cooperation between subclasses of Ly+ cells in the generation of killer activity. J Exp Med. 1975 Jun 1;141(6):1390–1399. doi: 10.1084/jem.141.6.1390. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Cerottini J. C., Brunner K. T. Cell-mediated cytotoxicity, allograft rejection, and tumor immunity. Adv Immunol. 1974;18:67–132. doi: 10.1016/s0065-2776(08)60308-9. [DOI] [PubMed] [Google Scholar]
  7. Cerottini J. C., Engers H. D., Macdonald H. R., Brunner T. Generation of cytotoxic T lymphocytes in vitro. I. Response of normal and immune mouse spleen cells in mixed leukocyte cultures. J Exp Med. 1974 Sep 1;140(3):703–717. doi: 10.1084/jem.140.3.703. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. David C. S., Frelinger J. A., Shreffler D. C. New lymphocyte antigens controlled by the Ir-IgG region of the H-2 gene complex. Transplantation. 1974 Jan 1;17(1):122–125. [PubMed] [Google Scholar]
  9. David C. S., Shreffler D. C., Frelinger J. A. New lymphocyte antigen system (Lna) controlled by the Ir region of the mouse H-2 complex. Proc Natl Acad Sci U S A. 1973 Sep;70(9):2509–2514. doi: 10.1073/pnas.70.9.2509. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Eijsvoogel V. P., du Bois M. J., Meinesz A., Bierhorst-Eijlander A., Zeylemaker W. P., Schellekens P. T. The specificty and the activation mechanism of cell-mediated lympholysis (CML) in man. Transplant Proc. 1973 Dec;5(4):1675–1678. [PubMed] [Google Scholar]
  11. Feldman M., Cohen I. R., Wekerle H. T-cell mediated immunity in vitro: an analysis of antigen recognition and target cell lysis. Transplant Rev. 1972;12:57–90. doi: 10.1111/j.1600-065x.1972.tb00053.x. [DOI] [PubMed] [Google Scholar]
  12. Frelinger J. A., Murphy D. B., McCormick J. F. Tla types of H-2 congenic and recombinant mice. Transplantation. 1974 Sep;18(3):292–294. [PubMed] [Google Scholar]
  13. Götze D., Reisfeld R. A., Klein J. Serologic evidence for antigens controlled by the Ir region in mice. J Exp Med. 1973 Oct 1;138(4):1003–1008. doi: 10.1084/jem.138.4.1003. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Hauptfeld V., Hauptfeld M., Klein J. Tissue distribution of I region-associated antigens in the mouse. J Immunol. 1974 Jul;113(1):181–188. [PubMed] [Google Scholar]
  15. Hauptfeld V., Klein D., Klein J. Serological identification of an Ir-region product. Science. 1973 Jul 13;181(4095):167–169. doi: 10.1126/science.181.4095.167. [DOI] [PubMed] [Google Scholar]
  16. Häyry P., Andersson L. C., Nordling S., Virolainen M. Allograft response in vitro. Transplant Rev. 1972;12:91–140. doi: 10.1111/j.1600-065x.1972.tb00054.x. [DOI] [PubMed] [Google Scholar]
  17. Klein J., Bailey D. W. Histocompatibility differences in wild mice; further evidence for the existence of deme structure in natural populations of the house mouse. Genetics. 1971 Jun;68(2):287–297. doi: 10.1093/genetics/68.2.287. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Lonai P., McDevitt H. O. I-region genes are expressed on T and B lymphocytes. Studies of the mixed lymphocyte reaction (MLR). J Exp Med. 1974 Nov 1;140(5):1317–1323. doi: 10.1084/jem.140.5.1317. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. MacDonald H. R., Phillips R. A., Miller R. G. Allograft immunity in the mouse. II. Physical studies of the development of cytotoxic effector cells from their immediate progenitors. J Immunol. 1973 Aug;111(2):575–589. [PubMed] [Google Scholar]
  20. Meo T., David C. S., Nabholz M., Miggiano V., Shreffler D. C. Demonstration by MLR test of a previously unsuspected intra-H-2 crossover in the B10.HTT strain: implications concerning location of MLR determinants in the Ir region. Transplant Proc. 1973 Dec;5(4):1507–1510. [PubMed] [Google Scholar]
  21. Miller R. G., Phillips R. A. Separation of cells by velocity sedimentation. J Cell Physiol. 1969 Jun;73(3):191–201. doi: 10.1002/jcp.1040730305. [DOI] [PubMed] [Google Scholar]
  22. Nabholz M., Vives J., Young H. M., Meo T., Miggiano V., Rijnbeek A., Shreffler D. C. Cell-mediated cell lysis in vitro: genetic control of killer cell production and target specificities in the mouse. Eur J Immunol. 1974 May;4(5):378–387. doi: 10.1002/eji.1830040514. [DOI] [PubMed] [Google Scholar]
  23. Röllinghoff M., Pfizenmeier K., Trostmann H., Wagner H. T cell proliferation in the mixed lymphocyte culture does not necessarily result in the generation of cytotoxic T effector cells. Eur J Immunol. 1975 Aug;5(8):560–564. doi: 10.1002/eji.1830050811. [DOI] [PubMed] [Google Scholar]
  24. Schendel D. J., Alter B. J., Bach F. H. The involvement of LD- and SD-region differences in MLC and CML: a three-cell experiment. Transplant Proc. 1973 Dec;5(4):1651–1655. [PubMed] [Google Scholar]
  25. Schendel D. J., Bach F. H. Genetic control of cell-mediated lympholysis in mouse. J Exp Med. 1974 Dec 1;140(6):1534–1546. doi: 10.1084/jem.140.6.1534. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Shreffler D. C., David C. S. The H-2 major histocompatibility complex and the I immune response region: genetic variation, function, and organization. Adv Immunol. 1975;20:125–195. doi: 10.1016/s0065-2776(08)60208-4. [DOI] [PubMed] [Google Scholar]
  27. Wagner H. Cell-mediated immune responses in vitro: interaction of thymus-derived cells during cytotoxic allograft responses in vitro. Science. 1973 Sep 21;181(4105):1170–1172. doi: 10.1126/science.181.4105.1170. [DOI] [PubMed] [Google Scholar]
  28. Wagner H., Röllinghoff M., Nossal G. J. T-cell-mediated immune responses induced in vitro: a probe for allograft and tumor immunity. Transplant Rev. 1973;17(0):3–36. doi: 10.1111/j.1600-065x.1973.tb00122.x. [DOI] [PubMed] [Google Scholar]
  29. Wagner H., Röllinghoff M., Nossal G. J. T-cell-mediated immune responses induced in vitro: a probe for allograft and tumor immunity. Transplant Rev. 1973;17(0):3–36. doi: 10.1111/j.1600-065x.1973.tb00122.x. [DOI] [PubMed] [Google Scholar]
  30. Wagner H. Synergy during in vitro cytotoxic allograft responses. I. Evidence for cell interaction between thymocytes and peripheral T cells. J Exp Med. 1973 Dec 1;138(6):1379–1397. doi: 10.1084/jem.138.6.1379. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from The Journal of Experimental Medicine are provided here courtesy of The Rockefeller University Press

RESOURCES