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. 1975 Dec 1;142(6):1462–1476. doi: 10.1084/jem.142.6.1462

The selective eosinophil chemotactic activity of histamine

PMCID: PMC2190076  PMID: 450

Abstract

Histamine diphosphate was shown to selectively attract human eosinophils from mixed granulocyte populations when over 20% eosinophils were used in a modified Boyden chamber chemotactic assay system. This effect of histamine is abolished by incubation with diamine oxidase (histaminase) and was generated by decarboxylation of L- histidine. A linear dose dependent increase in eosinophil migration was observed between 3 X 10(-7) M and 1.25 X 10(-6) M, while higher concentrations of histamine inhibited the migration of eosinophils. The attractant activity of histamine was not inhibited by H-1 or H-2 receptor antagonists, however, the inhibition of migration observed at higher histamine concentrations was reversed by metiamine, an H-2 receptor antagonist. The effects of histamine upon eosinophil migration were demonstrable using three different assays: (a) counting cells that had traversed 5-mum pore, 12-mum thick polycarbonate filters, (b) counting cells that had migrated various distances into a 3-mum pore, 145-mum cellulose nitrate filters, or (c) measuring the number of cells that had traversed an upper polycarbonate filter and migrated into a lower cellulose nitrate filter using 15Cr-labeled cells. The ability of histamine to enhance eosinophil migration was shown to be dependent upon the presence of a concentration gradient; histamine did not cause a dose-dependent increase in random motility. Furthermore, preincubation of the eosinophils with histamine deactivate the cells to further stimulation by histamine or by C5a. It is concluded that in low doses histamine is a chemoattractant for human eosinophils, while in higher doses histamine inhibits eosinophil migration. These observations may relate to the influx and localization of eosinophils in immediate hypersensitivity reactions.

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Selected References

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  1. Becker E. L. The relationship of the chemotactic behavior of the complement-derived factors, C3a, C5a, and C567, and a bacterial chemotactic factor to their ability to activate the proesterase 1 of rabbit polymorphonuclear leukocytes. J Exp Med. 1972 Feb 1;135(2):376–387. doi: 10.1084/jem.135.2.376. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Beutler E. Cellular mosaicism and heterogeneity in red cell disorders. Am J Med. 1966 Nov;41(5):724–733. doi: 10.1016/0002-9343(66)90033-7. [DOI] [PubMed] [Google Scholar]
  3. Black J. W., Duncan W. A., Durant C. J., Ganellin C. R., Parsons E. M. Definition and antagonism of histamine H 2 -receptors. Nature. 1972 Apr 21;236(5347):385–390. doi: 10.1038/236385a0. [DOI] [PubMed] [Google Scholar]
  4. Gallin J. I., Clark R. A., Kimball H. R. Granulocyte chemotaxis: an improved in vitro assay employing 51 Cr-labeled granulocytes. J Immunol. 1973 Jan;110(1):233–240. [PubMed] [Google Scholar]
  5. Gallin J. I., Rosenthal A. S. The regulatory role of divalent cations in human granulocyte chemotaxis. Evidence for an association between calcium exchanges and microtubule assembly. J Cell Biol. 1974 Sep;62(3):594–609. doi: 10.1083/jcb.62.3.594. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Ishizaka T., De Bernardo R., Tomioka H., Lichtenstein L. M., Ishizaka K. Identification of basophil granulocytes as a site of allergic histamine release. J Immunol. 1972 Apr;108(4):1000–1008. [PubMed] [Google Scholar]
  7. Ishizaka T., Ishizaka K., Orange R. P., Austen K. F. The capacity of human immunoglobulin E to mediate the release of histamine and slow reacting substance of anaphylaxis (SRS-A) from monkey lung. J Immunol. 1970 Feb;104(2):335–343. [PubMed] [Google Scholar]
  8. Kay A. B., Austen K. F. The IgE-mediated release of an eosinophil leukocyte chemotactic factor from human lung. J Immunol. 1971 Sep;107(3):899–902. [PubMed] [Google Scholar]
  9. Kay A. B., Stechschulte D. J., Austen K. F. An eosinophil leukocyte chemotactic factor of anaphylaxis. J Exp Med. 1971 Mar 1;133(3):602–619. doi: 10.1084/jem.133.3.602. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Keller H. U., Borel J. F., Wilkinson P. C., Hess M. W., Cottier H. Re-assessment of Boyden's technique for measuring chemotaxis. J Immunol Methods. 1972 Jan;1(2):165–168. doi: 10.1016/0022-1759(72)90043-9. [DOI] [PubMed] [Google Scholar]
  11. Lewis R. A., Goetzl E. J., Wasserman S. I., Valone F. H., Rubin R. H., Austen K. F. The release of four mediators of immediate hypersensitivity from human leukemic basophils. J Immunol. 1975 Jan;114(1 Pt 1):87–92. [PubMed] [Google Scholar]
  12. MOTA I., BERALDO W. T., FERRI A. G., JUNQUEIRA L. C. Intracellular distribution of histamine. Nature. 1954 Oct 9;174(4432):698–698. doi: 10.1038/174698a0. [DOI] [PubMed] [Google Scholar]
  13. Parish W. E. Eosinophilia. 3. The anaphylactic release from isolated human basophils of a substance that selectively attracts eosinophils. Clin Allergy. 1972 Dec;2(4):381–390. doi: 10.1111/j.1365-2222.1972.tb01302.x. [DOI] [PubMed] [Google Scholar]
  14. Parish W. E. Substances that attract eosinophils in vitro and in vivo, and that elicit blood eosinophilia. Antibiot Chemother (1971) 1974;19:233–270. doi: 10.1159/000395434. [DOI] [PubMed] [Google Scholar]
  15. Plaut M., Lichtenstein L. M., Henney C. S. Properties of a subpopulation of T cells bearing histamine receptors. J Clin Invest. 1975 Apr;55(4):856–874. doi: 10.1172/JCI107997. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Pruzansky J. J., Patterson R. Histamine in human leuccytes. Localization of histamine and beta glucuronidase in human leucocytes. Int Arch Allergy Appl Immunol. 1970;37(1):98–103. doi: 10.1159/000230224. [DOI] [PubMed] [Google Scholar]
  17. RILEY J. F., WEST G. B. The presence of histamine in tissue mast cells. J Physiol. 1953 Jun 29;120(4):528–537. doi: 10.1113/jphysiol.1953.sp004915. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. ROCHA E SILVA M., BERALDO W. T., ROSENFELD G. Bradykinin, a hypotensive and smooth muscle stimulating factor released from plasma globulin by snake venoms and by trypsin. Am J Physiol. 1949 Feb;156(2):261–273. doi: 10.1152/ajplegacy.1949.156.2.261. [DOI] [PubMed] [Google Scholar]
  19. Schiffmann E., Corcoran B. A., Wahl S. M. N-formylmethionyl peptides as chemoattractants for leucocytes. Proc Natl Acad Sci U S A. 1975 Mar;72(3):1059–1062. doi: 10.1073/pnas.72.3.1059. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Schiffmann E., Showell H. V., Corcoran B. A., Ward P. A., Smith E., Becker E. L. The isolation and partial characterization of neutrophil chemotactic factors from Escherichia coli. J Immunol. 1975 Jun;114(6):1831–1837. [PubMed] [Google Scholar]
  21. Snyderman R., Altman L. C., Hausman M. S., Mergenhagen S. E. Human mononuclear leukocyte chemotaxis: a quantitative assay for humoral and cellular chemotactic factors. J Immunol. 1972 Mar;108(3):857–860. [PubMed] [Google Scholar]
  22. Zigmond S. H., Hirsch J. G. Leukocyte locomotion and chemotaxis. New methods for evaluation, and demonstration of a cell-derived chemotactic factor. J Exp Med. 1973 Feb 1;137(2):387–410. doi: 10.1084/jem.137.2.387. [DOI] [PMC free article] [PubMed] [Google Scholar]

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