Skip to main content
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1985 Apr;162(1):445–447. doi: 10.1128/jb.162.1.445-447.1985

Acylated proteins in Acholeplasma laidlawii.

C E Dahl, N C Sacktor, J S Dahl
PMCID: PMC219011  PMID: 3980443

Abstract

The covalent modification of membrane proteins by long-chain fatty acids was determined in two strains of Acholeplasma laidlawii by one-dimensional gel electrophoresis of radiolabeled membranes. Of the more than 50 membrane polypeptides detected, approximately 30 were labeled with [3H]palmitate, whereas covalent binding of [3H]oleate to membrane proteins could not be demonstrated. We suggest that in these wall-less bacteria, membrane protein acylation with saturated fatty acids may serve to ensure the structural integrity of the membrane.

Full text

PDF
445

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Cham B. E., Knowles B. R. A solvent system for delipidation of plasma or serum without protein precipitation. J Lipid Res. 1976 Mar;17(2):176–181. [PubMed] [Google Scholar]
  2. Dahl C. E., Dahl J. S., Bloch K. Proteolipid formation in Mycoplasma capricolum. Influence of cholesterol on unsaturated fatty acid acylation of membrane proteins. J Biol Chem. 1983 Oct 10;258(19):11814–11818. [PubMed] [Google Scholar]
  3. Dahl C. E., Dahl J. S. Phospholipids as acyl donors to membrane proteins of Mycoplasma capricolum. J Biol Chem. 1984 Sep 10;259(17):10771–10776. [PubMed] [Google Scholar]
  4. Dahl J. S., Dahl C. E., Bloch K. Sterols in membranes: growth characteristics and membrane properties of Mycoplasma capricolum cultured on cholesterol and lanosterol. Biochemistry. 1980 Apr 1;19(7):1467–1472. doi: 10.1021/bi00548a032. [DOI] [PubMed] [Google Scholar]
  5. Henning R., Lange-Mutschler J. Tightly associated lipids may anchor SV40 large T antigen in plasma membrane. Nature. 1983 Oct 20;305(5936):736–738. doi: 10.1038/305736a0. [DOI] [PubMed] [Google Scholar]
  6. Huang L., Haug A. Regulation of membrane lipid fluidity in Acholeplasma laidlawii: effect of carotenoid pigment content. Biochim Biophys Acta. 1974 Jun 29;352(3):361–370. doi: 10.1016/0005-2736(74)90228-4. [DOI] [PubMed] [Google Scholar]
  7. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  8. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  9. Magee A. I., Schlesinger M. J. Fatty acid acylation of eucaryotic cell membrane proteins. Biochim Biophys Acta. 1982 Nov 30;694(3):279–289. doi: 10.1016/0304-4157(82)90008-9. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Mizushima S. Post-translational modification and processing of outer membrane prolipoproteins in Escherichia coli. Mol Cell Biochem. 1984;60(1):5–15. doi: 10.1007/BF00226297. [DOI] [PubMed] [Google Scholar]
  11. Rottem S., Markowitz O. Carotenoids acts as reinforcers of the Acholeplasma laidlawii lipid bilayer. J Bacteriol. 1979 Dec;140(3):944–948. doi: 10.1128/jb.140.3.944-948.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Schmidt M. F., Bracha M., Schlesinger M. J. Evidence for covalent attachment of fatty acids to Sindbis virus glycoproteins. Proc Natl Acad Sci U S A. 1979 Apr;76(4):1687–1691. doi: 10.1073/pnas.76.4.1687. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Schmidt R. A., Schneider C. J., Glomset J. A. Evidence for post-translational incorporation of a product of mevalonic acid into Swiss 3T3 cell proteins. J Biol Chem. 1984 Aug 25;259(16):10175–10180. [PubMed] [Google Scholar]
  14. Smith P. F., Henrikson C. V. Growth inhibition of Mycoplasma by inhibitors of polyterpene biosynthesis and its reversal by cholesterol. J Bacteriol. 1966 May;91(5):1854–1858. doi: 10.1128/jb.91.5.1854-1858.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Stanbridge E. J., Weiss R. L. Mycoplasma capping on lymphocytes. Nature. 1978 Dec 7;276(5688):583–587. doi: 10.1038/276583a0. [DOI] [PubMed] [Google Scholar]
  16. Wise K. S., Cassell G. H., Action R. T. Selective association of murine T lymphoblastoid cell surface alloantigens with Mycoplasma hyorhinis. Proc Natl Acad Sci U S A. 1978 Sep;75(9):4479–4483. doi: 10.1073/pnas.75.9.4479. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Wu H. C., Lai J. S., Hayashi S., Giam C. Z. Biogenesis of membrane lipoproteins in Escherichia coli. Biophys J. 1982 Jan;37(1):307–315. doi: 10.1016/S0006-3495(82)84679-1. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES