Skip to main content
PMC home page
The Journal of Experimental Medicine logoLink to The Journal of Experimental Medicine
. 1976 Mar 1;143(3):482–496. doi: 10.1084/jem.143.3.482

Leukemia in AKR mice. I. Effects of leukemic cells on antibody-forming potential of syngeneic and allogeneic normal cells

PMCID: PMC2190145  PMID: 942993

Abstract

Cells from the spleen, thymus, lymph node, and liver of leukemic AKR mice suppress in vitro antibody responses of normal syngeneic and semiallogeneic cells. This suppression can be mediated by irradiated leukemic cells, requires cell contact between leukemic and normal cells, and may occur at any time during the in vitro culture period. Leukemic AKR cells do not suppress antibody responses of allogeneic cells, even when allogeneic cells have H-2 or background genes homologous with AKR. Leukemic cells do, however, suppress cells that are unable to respond allogeneically to leukemic AKR cells, such as cells of the F1s of AKR. Suppression of normal AKR antibody responses by leukemic AKR cells may be overcome by addition of irradiated allogeneic cells. The fact that leukemic AKR cells are able to suppress normal lymphocyte responses may be of significance in pathogenesis of leukemia in these mice.

Full Text

The Full Text of this article is available as a PDF (979.8 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson R. J., McBride C. M., Hersh E. M. Lymphocyte blastogenic responses to cultured allogeneic tumor cells in vitro. Cancer Res. 1972 May;32(5):988–992. [PubMed] [Google Scholar]
  2. Baldwin R. W., Price M. R., Robins R. A. Inhibition of hepatoma-immune lymph-node cell cytotoxicity by tumour-bearer serum, and solubilized hepatoma antigen. Int J Cancer. 1973 May;11(3):527–535. doi: 10.1002/ijc.2910110304. [DOI] [PubMed] [Google Scholar]
  3. Blair P. B., Lane M. A., Yagi M. J. Blocking of spleen cell activity against target mammary tumor cells by viral antigens. J Immunol. 1975 Jul;115(1):190–194. [PubMed] [Google Scholar]
  4. Bortin M. M., Rimm A. A., Saltzstein E. C. Graft versus leukemia: quantification of adoptive immunotherapy in murine leukemia. Science. 1973 Feb 23;179(4075):811–813. doi: 10.1126/science.179.4075.811. [DOI] [PubMed] [Google Scholar]
  5. Calkins C. E., Golub E. S. Direct demonstration of lymphocyte--macrophage cooperation in the absence of physical contact between the two cell types. Cell Immunol. 1972 Dec;5(4):579–586. doi: 10.1016/0008-8749(72)90108-6. [DOI] [PubMed] [Google Scholar]
  6. Cunningham A. J., Szenberg A. Further improvements in the plaque technique for detecting single antibody-forming cells. Immunology. 1968 Apr;14(4):599–600. [PMC free article] [PubMed] [Google Scholar]
  7. Devlin R. G., Baugh L. E., McCurdy J. D., Baronowsky P. E. Studies on antilymphocytic autoimmune reactions in L1210 bearing mice. Cell Immunol. 1975 May;17(1):156–164. doi: 10.1016/s0008-8749(75)80016-5. [DOI] [PubMed] [Google Scholar]
  8. Eggers A. E., Wunderlich J. R. Suppressor cells in tumor-bearing mice capable of nonspecific blocking of in vitro immunization against transplant antigens. J Immunol. 1975 May;114(5):1554–1556. [PubMed] [Google Scholar]
  9. Festenstein H. Immunogenetic and biological aspects of in vitro lymphocyte allotransformation (MLR) in the mouse. Transplant Rev. 1973;15:62–88. doi: 10.1111/j.1600-065x.1973.tb00111.x. [DOI] [PubMed] [Google Scholar]
  10. Frey-Wettstein M., Hays E. F. Immune response in preleukemic mice. Infect Immun. 1970 Oct;2(4):398–403. doi: 10.1128/iai.2.4.398-403.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Gorczynski R. M. Immunity to murine sarcoma virus-inducted tumors. II. Suppression of T cell-mediated immunity by cells from progressor animals. J Immunol. 1974 May;112(5):1826–1838. [PubMed] [Google Scholar]
  12. Gorczynski R. M., Kilburn D. G., Knight R. A., Norbury C., Parker D. C., Smith J. B. Nonspecific and specific immunosuppression in tumour-bearing mice by soluble immune complexes. Nature. 1975 Mar 13;254(5496):141–143. doi: 10.1038/254141a0. [DOI] [PubMed] [Google Scholar]
  13. Hellström I., Hellström K. E. Studies on cellular immunity and its serum mediated inhibition in Moloney-virus-induced mouse sarcomas. Int J Cancer. 1969 Sep 15;4(5):587–600. doi: 10.1002/ijc.2910040503. [DOI] [PubMed] [Google Scholar]
  14. Hrsak I., Marotti T. Mode of immunosuppressive action of Ehrlich ascitic fluid. J Natl Cancer Inst. 1974 Oct;53(4):1113–1119. doi: 10.1093/jnci/53.4.1113. [DOI] [PubMed] [Google Scholar]
  15. Jacobs D. M., Morrison D. C. Stimulation of a T-independent primary anti-hapten response in vitro by TNP-lipopolysaccharide (TNP-LPS). J Immunol. 1975 Jan;114(1 Pt 2):360–364. [PubMed] [Google Scholar]
  16. Kamo I., Patel C., Kateley J., Friedman H. Immunosuppression induced in vitro by mastocytoma tumor cells and cell-free extracts. J Immunol. 1975 Jun;114(6):1749–1756. [PubMed] [Google Scholar]
  17. Kirchner H., Muchmore A. V., Chused T. M., Holden H. T., Herberman R. B. Inhibition of proliferation of lymphoma cells and T lymphocytes by suppressor cells from spleens of tumor-bearing mice. J Immunol. 1975 Jan;114(1 Pt 1):206–210. [PubMed] [Google Scholar]
  18. Martig R. J., Tribble J. L. Cell-Mediated Immune Responses of Preleukemic AKR Mice. Infect Immun. 1974 Nov;10(5):1018–1022. doi: 10.1128/iai.10.5.1018-1022.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Metcalf D., Moulds R. Immune responses in preleukaemic and leukaemic AKR mice. Int J Cancer. 1967 Jan 15;2(1):53–58. doi: 10.1002/ijc.2910020109. [DOI] [PubMed] [Google Scholar]
  20. Mishell R. I., Dutton R. W. Immunization of dissociated spleen cell cultures from normal mice. J Exp Med. 1967 Sep 1;126(3):423–442. doi: 10.1084/jem.126.3.423. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Mortensen R. F., Ceglowski W. S., Friedman H. Leukemia virus-induced immunosuppression. X. Depression of T cell-mediated cytotoxicity after infection of mice with Friend leukemia virus. J Immunol. 1974 Jun;112(6):2077–2086. [PubMed] [Google Scholar]
  22. Nagaya H. Thymus function in spontaneous lymphoid leukemia. II. In vitro response of "preleukemic" and leukemic thymus cells to mitogens. J Immunol. 1973 Oct;111(4):1052–1060. [PubMed] [Google Scholar]
  23. Nowotny A., Grohsman J., Abdelnoor A., Rote N., Yang C., Waltersdorff R. Escape of TA3 tumors from allogeneic immune rejection: theory and experiments. Eur J Immunol. 1974 Feb;4(2):73–78. doi: 10.1002/eji.1830040204. [DOI] [PubMed] [Google Scholar]
  24. Peled A., Haran-Ghera N. The cellular basis of immunosuppression caused by the radiation leukaemia virus. Immunology. 1974 Feb;26(2):323–329. [PMC free article] [PubMed] [Google Scholar]
  25. Pikovski M. A., Ziffroni-Gallon Y., Witz I. P. Suppression of immune response to sheep red blood cells in mice treated with preparations of a tumor cell component and in tumor-bearing mice. Eur J Immunol. 1976 Jul;5(7):447–450. doi: 10.1002/eji.1830050704. [DOI] [PubMed] [Google Scholar]
  26. Ram M. D., Kohn R. R., Novak D. Antigen distribution in AKR mouse leukemia. J Natl Cancer Inst. 1974 May;52(5):1505–1514. doi: 10.1093/jnci/52.5.1505. [DOI] [PubMed] [Google Scholar]
  27. Ram M. D., Kohn R. R., Novak D. Immune response to sheep red cells in AKR mouse leukemia. Am J Pathol. 1973 Jul;72(1):39–52. [PMC free article] [PubMed] [Google Scholar]
  28. Rittenberg M. B., Pratt K. L. Antitrinitrophenyl (TNP) plaque assay. Primary response of Balb/c mice to soluble and particulate immunogen. Proc Soc Exp Biol Med. 1969 Nov;132(2):575–581. doi: 10.3181/00379727-132-34264. [DOI] [PubMed] [Google Scholar]
  29. Sjögren H. O., Hellström I., Bansal S. C., Hellström K. E. Suggestive evidence that the "blocking antibodies" of tumor-bearing individuals may be antigen--antibody complexes. Proc Natl Acad Sci U S A. 1971 Jun;68(6):1372–1375. doi: 10.1073/pnas.68.6.1372. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Stockert E., Old L. J., Boyse E. A. The G-IX system. A cell surface allo-antigen associated with murine leukemia virus; implications regarding chromosomal integration of the viral genome. J Exp Med. 1971 Jun 1;133(6):1334–1355. doi: 10.1084/jem.133.6.1334. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Tanapatchaiyapong P., Zolla S. Humoral immunosuppressive substance in mice bearing plasmacytomas. Science. 1974 Nov 22;186(4165):748–750. doi: 10.1126/science.186.4165.748. [DOI] [PubMed] [Google Scholar]
  32. Weislow O. S., Wheelock E. F. Depression of humoral immunity to sheep erythrocytes in vitro by Friend virus leukemic spleen cells: induction of resistance by statolon. J Immunol. 1975 Jan;114(1 Pt 1):211–215. [PubMed] [Google Scholar]
  33. Wong A., Mankovitz R., Kennedy J. C. Immunosuppressive and immunostimulatory factors produced by malignant cells in vitro. Int J Cancer. 1974 Apr 15;13(4):530–542. doi: 10.1002/ijc.2910130413. [DOI] [PubMed] [Google Scholar]
  34. Zatz M. M., White A., Goldstein A. L. Lymphocyte population of AKR-J mice. II. Effect of leukemogenesis on migration patterns, response to PHA, and expression of theta antigen. J Immunol. 1973 Nov;111(5):1519–1525. [PubMed] [Google Scholar]

Articles from The Journal of Experimental Medicine are provided here courtesy of The Rockefeller University Press

RESOURCES