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. 1976 Apr 1;143(4):822–832. doi: 10.1084/jem.143.4.822

Gangliosides as markers for murine lymphocyte subpopulations

PMCID: PMC2190168  PMID: 56418

Abstract

Antibodies to GM1 ganglioside were used to study murine lymphocyte populations. In A, AKR, and BALB/c mice, anti-GM1 reacts with thymocytes and peripheral T cells. This reactivity of anti-GM1, studied by immunofluorescence, is independent of Thy-1 type and appears to be related to the reactivity of cross-reacting antibodies to asialo GM1 and GD1b, rather than GM1 itself. In addition, a subpopulation of lymphocytes reacting with anti-GM1 and anti-immunoglobulin has been found in approximately 26% of the peripheral lymphocytes of C3H mice, nude mice, and nude heterozygotes. This subpopulation is found in small numbers in A, AKR, and BALB/c mice. These studies demonstrate that antibodies to a chemically defined antigen can be used to identify T cells in many strains of mice and may delineate previously unrecognized lymphocyte subpopulations.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Coutinho A., Gronowicz E. Genetical control of B-cell responses. III. Requirement for functional mitogenicity of the antigen in thymus-independent specific responses. J Exp Med. 1975 Apr 1;141(4):753–760. [PMC free article] [PubMed] [Google Scholar]
  2. Cuatrecasas P. Interaction of Vibrio cholerae enterotoxin with cell membranes. Biochemistry. 1973 Aug 28;12(18):3547–3558. doi: 10.1021/bi00742a031. [DOI] [PubMed] [Google Scholar]
  3. Esselman W. J., Miller H. C. Brain and thymus lipid inhibition of antibrain-associated theta-cytotoxicity. J Exp Med. 1974 Feb 1;139(2):445–450. doi: 10.1084/jem.139.2.445. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Gahmberg C. G., Hakomori S. I. External labeling of cell surface galactose and galactosamine in glycolipid and glycoprotein of human erythrocytes. J Biol Chem. 1973 Jun 25;248(12):4311–4317. [PubMed] [Google Scholar]
  5. Greenberg R. S., Zatz M. M. Spontaneous AKR lymphoma with T and B-cell characteristics. Nature. 1975 Sep 25;257(5524):314–316. doi: 10.1038/257314a0. [DOI] [PubMed] [Google Scholar]
  6. Holmgren J., Lindholm L., Lönnroth I. Interaction of cholera toxin and toxin derivatives with lymphocytes. I. Binding properties and interference with lectin-induced cellular stimulation. J Exp Med. 1974 Apr 1;139(4):801–819. doi: 10.1084/jem.139.4.801. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Komuro K., Boyse E. A. Induction of T lymphocytes from precursor cells in vitro by a product of the thymus. J Exp Med. 1973 Aug 1;138(2):479–482. doi: 10.1084/jem.138.2.479. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kucich U. N., Bennett J. C., Johnson B. J. The protein nature of the Thy-1.2 alloantigen as expressed by the murin lymphoblastoid line S-49.1 TB-2-3. J Immunol. 1975 Sep;115(3):626–630. [PubMed] [Google Scholar]
  9. Letarte-Muirhead M., Barclay A. N., Williams A. F. Purification of the Thy-1 molecule, a major cell-surface glycoprotein of rat thymocytes. Biochem J. 1975 Dec;151(3):685–697. doi: 10.1042/bj1510685. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Miller H. C., Esselman W. J. Identification of theta-bearing T-cells derived from bone marrow cells treated with thymic factor. Ann N Y Acad Sci. 1975 Feb 28;249:54–60. doi: 10.1111/j.1749-6632.1975.tb29057.x. [DOI] [PubMed] [Google Scholar]
  11. Naiki M., Marcus D. M., Ledeen R. Properties of antisera to ganglioside GM1 and asialo GM1. J Immunol. 1974 Jul;113(1):84–93. [PubMed] [Google Scholar]
  12. Raff M. C. Two distinct populations of peripheral lymphocytes in mice distinguishable by immunofluorescence. Immunology. 1970 Oct;19(4):637–650. [PMC free article] [PubMed] [Google Scholar]
  13. Reif A. E., Allen J. M. Mouse thymic iso-antigens. Nature. 1966 Jan 29;209(5022):521–523. doi: 10.1038/209521b0. [DOI] [PubMed] [Google Scholar]
  14. Rubin B., Trier L., Hertel-Wulff B., Rumler B. Selective retention of T cell precursors in bone marrow. Cell populations after filtration through anti-immunoglobulin columns. Cell Immunol. 1975 Apr;16(2):315–329. doi: 10.1016/0008-8749(75)90121-5. [DOI] [PubMed] [Google Scholar]
  15. Révész T., Greaves M. Ligand-induced redistribution of lymphocyte membrane ganglioside GM1. Nature. 1975 Sep 11;257(5522):103–106. doi: 10.1038/257103a0. [DOI] [PubMed] [Google Scholar]
  16. Vitetta E. S., Boyse E. A., Uhr J. W. Isolation and characterization of a molecular complex containing Thy-1 antigen from the surface of murine thymocytes and T cells. Eur J Immunol. 1973 Jul;3(7):446–453. doi: 10.1002/eji.1830030714. [DOI] [PubMed] [Google Scholar]
  17. Watson J., Riblet R. Genetic control of responses to bacterial lipopolysaccharides in mice. I. Evidence for a single gene that influences mitogenic and immunogenic respones to lipopolysaccharides. J Exp Med. 1974 Nov 1;140(5):1147–1161. doi: 10.1084/jem.140.5.1147. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Yutoku M., Grossberg A. L., Pressman D. The expression on mouse lymphoid cells of Th-B, an antigen common to mouse B cells and thymus cells. J Immunol. 1975 Jul;115(1):69–74. [PubMed] [Google Scholar]

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