Skip to main content
PMC home page
The Journal of Experimental Medicine logoLink to The Journal of Experimental Medicine
. 1976 Apr 1;143(4):846–860. doi: 10.1084/jem.143.4.846

Idiotypic analysis of lymphocytes in vitro. I. Specificity and heterogeneity of B and T lymphocytes reactive with anti-idiotypic antibody

PMCID: PMC2190175  PMID: 768405

Abstract

Guinea pig anti-idiotypic antibodies (anti-Id) of the IgG1 class, directed to an A/J antibody to Group A streptococcal carbohydrate (A- CHO), or directed to a BALB/c myeloma protein that binds the same antigen, stimulate B-precursor cells as well as T-helper cells when injected into mice of the appropriate strain. The strain-specific induction of both precursor and helper activity was detected by in vitro secondary responses of primed spleen cells to A-CHO or to 2,4,6- trinitrophenyl (TNP) upon challenge with Group A streptococcal vaccine (Strep.A) or with TNP-Strep.A, respectively. B- and T-cell populations primed with anti-Id were uniform with respect to the binding of antigen and of anti-Id. This was in contrast to cells primed with Strep.A, which were heterogenous. Taken together, B and T cells that possess the same antigen-binding specificity share idiotypic determinants, reveal the same idiotypic polymorphism, and may display similar degrees of heterogeneity with respect to the binding of antigen and anti-Id. Since the anti-Id used in this study detect Id determinants associated with the heavy chain of the variable region of mouse antibodies, the data suggest that this region of the immunoglobulin molecule is shared between T- and B-cell antigen receptors.

Full Text

The Full Text of this article is available as a PDF (974.2 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BENACERRAF B., OVARY Z., BLOCH K. J., FRANKLIN E. C. Properties of guinea pig 7S antibodies. I. Electrophoretic separation of two types of guinea pig 7S antibodies. J Exp Med. 1963 Jun 1;117:937–949. doi: 10.1084/jem.117.6.937. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Binz H., Lindenmann J., Wigzell H. Cell-bound receptors for alloantigens on normal lymphocytes. II. Antialloantibody serum contains specific factors reacting with relevant immunocompetent T lymphocytes. J Exp Med. 1974 Sep 1;140(3):731–741. doi: 10.1084/jem.140.3.731. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Binz H., Wigzell H. Shared idiotypic determinants on B and T lymphocytes reactive against the same antigenic determinants. I. Demonstration of similar or identical idiotypes on IgG molecules and T-cell receptors with specificity for the same alloantigens. J Exp Med. 1975 Jul 1;142(1):197–211. doi: 10.1084/jem.142.1.197. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Binz H., Wigzell H. Shared idiotypic determinants on B and T lymphocytes reactive against the same antigenic determinants. IV. Isolation of two groups of naturally occurring, idiotypic molecules with specific antigen-binding activity in the serum and urine of normal rats. Scand J Immunol. 1975 Sep;4(5-6):591–600. doi: 10.1111/j.1365-3083.1975.tb02665.x. [DOI] [PubMed] [Google Scholar]
  5. Briles D. E., Davie J. M. Clonal dominance. I. Restricted nature of the IgM antibody response to group A streptococcal carbohydrate in mice. J Exp Med. 1975 Jun 1;141(6):1291–1307. doi: 10.1084/jem.141.6.1291. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Coligan J. E., Schnute W. C., Jr, Kindt T. J. Immunochemical and chemical studies on streptococcal group-specific carbohydrates. J Immunol. 1975 Jun;114(6):1654–1658. [PubMed] [Google Scholar]
  7. Douglas T. C. Occurrence of a theta-like antigen in rats. J Exp Med. 1972 Nov 1;136(5):1054–1062. doi: 10.1084/jem.136.5.1054. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Eichmann K., Berek C. Mendelian segregation of a mouse antibody idiotype. Eur J Immunol. 1973 Sep;3(9):599–601. doi: 10.1002/eji.1830030914. [DOI] [PubMed] [Google Scholar]
  9. Eichmann K., Greenblatt J. Relationships between relative binding affinity and electrophoretic behavior of rabbit antibodies to streptococcal carbohydrates. J Exp Med. 1971 Mar 1;133(3):424–441. doi: 10.1084/jem.133.3.424. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Eichmann K. Idiotype expression and the inheritance of mouse antibody clones. J Exp Med. 1973 Mar 1;137(3):603–621. doi: 10.1084/jem.137.3.603. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Eichmann K. Idiotype suppression. I. Influence of the dose and of the effector functions of anti-idiotypic antibody on the production of an idiotype. Eur J Immunol. 1974 Apr;4(4):296–302. doi: 10.1002/eji.1830040413. [DOI] [PubMed] [Google Scholar]
  12. Eichmann K. Idiotype suppression. II. Amplification of a suppressor T cell with anti-idiotypic activity. Eur J Immunol. 1975 Aug;5(8):511–517. doi: 10.1002/eji.1830050802. [DOI] [PubMed] [Google Scholar]
  13. Eichmann K. Idiotypic identity of antibodies to streptococcal carbohydrate in inbred mice. Eur J Immunol. 1972 Aug;2(4):301–307. doi: 10.1002/eji.1830020402. [DOI] [PubMed] [Google Scholar]
  14. Eichmann K., Rajewsky K. Induction of T and B cell immunity by anti-idiotypic antibody. Eur J Immunol. 1975 Oct;5(10):661–666. doi: 10.1002/eji.1830051002. [DOI] [PubMed] [Google Scholar]
  15. Hämmerling G. J., Black S. J., Berek C., Eichmann K., Rajewsky K. Idiotypic analysis of lymphocytes in vitro. II. Genetic control of T-helper cell responsiveness to anti-idiotypic antibody. J Exp Med. 1976 Apr 1;143(4):861–869. doi: 10.1084/jem.143.4.861. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Julius M. H., Simpson E., Herzenberg L. A. A rapid method for the isolation of functional thymus-derived murine lymphocytes. Eur J Immunol. 1973 Oct;3(10):645–649. doi: 10.1002/eji.1830031011. [DOI] [PubMed] [Google Scholar]
  17. Kristiansen T., Sundberg L., Porath J. Studies on blood group substances. II. Coupling of blood group substane A to hydroxyl-containing matrices, including aminoethyl cellulose and agarose. Biochim Biophys Acta. 1969 Jun 17;184(1):93–98. [PubMed] [Google Scholar]
  18. McCarty M. The streptococcal cell wall. Harvey Lect. 1971;65:73–96. [PubMed] [Google Scholar]
  19. Mishell R. I., Dutton R. W. Immunization of dissociated spleen cell cultures from normal mice. J Exp Med. 1967 Sep 1;126(3):423–442. doi: 10.1084/jem.126.3.423. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Paul W. E., Siskind G. W. Hapten specificity of cellular immune responses as compared with the specificity of serum anti-hapten antibody. Immunology. 1970 Jun;18(6):921–930. [PMC free article] [PubMed] [Google Scholar]
  21. Pavlovskis O., Slade H. D. Adsorption of 3H-fatty acid esters of streptococcal groups A and E cell wall polysaccharide antigens by red blood cells and their effect on hemagglutination. J Bacteriol. 1969 Nov;100(2):641–646. doi: 10.1128/jb.100.2.641-646.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Rajewsky K., Mohr R. Specificity and heterogeneity of helper T cells in the response to serum albumins in mice. Eur J Immunol. 1974 Feb;4(2):111–119. doi: 10.1002/eji.1830040210. [DOI] [PubMed] [Google Scholar]
  23. Rajewsky K., Roelants G. E., Askonas B. A. Carrier specificity and the allogeneic effect in mice. Eur J Immunol. 1972 Dec;2(6):592–598. doi: 10.1002/eji.1830020622. [DOI] [PubMed] [Google Scholar]
  24. Read S. E., Braun D. G. In vitro antibody response of primed rabbit peripheral blood lymphocytes to group A variant streptococcal polysaccharide. Eur J Immunol. 1974 Jun;4(6):422–426. doi: 10.1002/eji.1830040607. [DOI] [PubMed] [Google Scholar]
  25. Rittenberg M. B., Pratt K. L. Antitrinitrophenyl (TNP) plaque assay. Primary response of Balb/c mice to soluble and particulate immunogen. Proc Soc Exp Biol Med. 1969 Nov;132(2):575–581. doi: 10.3181/00379727-132-34264. [DOI] [PubMed] [Google Scholar]
  26. Schlossman S. F. Antigen recognition: the specificity of T cells involved in the cellular immune response. Transplant Rev. 1972;10:97–111. doi: 10.1111/j.1600-065x.1972.tb01540.x. [DOI] [PubMed] [Google Scholar]
  27. Vicari G., Sher A., Cohn M., Kabat E. A. Immunochemical studies on a mouse myeloma protein with specificity for certain beta-linked terminal residues of N-acetyl-D-glucosamine. Immunochemistry. 1970 Oct;7(10):829–838. doi: 10.1016/0019-2791(70)90059-5. [DOI] [PubMed] [Google Scholar]

Articles from The Journal of Experimental Medicine are provided here courtesy of The Rockefeller University Press

RESOURCES