Skip to main content
NCBI home page
The Journal of Experimental Medicine logoLink to The Journal of Experimental Medicine
. 1976 May 1;143(5):1042–1054. doi: 10.1084/jem.143.5.1042

Lymphocyte transformation induced by autologous cells. IV. Human T- lymphocyte proliferation induced by autologous or allogeneic non-T lymphocytes

PMCID: PMC2190192  PMID: 131174

Abstract

An autologous mixed lymphocyte reaction was demonstrated between T and non-T lymphocytes. Sheep erythrocyte rosetting was used to separate human lymphocytes into T and non-T lymphoid preparations. Non-T lymphocytes stimulated the proliferation of autologous T lymphocytes. The cell in this preparation that was most stimulatory had the characteristics of a K lymphocyte. The allogeneic mixed lymphocyte reaction was also shown to reflect the proliferation of T lymphocytes stimulated by allogeneic non-T lymphocytes. Proliferation of T lymphocytes in the allogeneic mixed lymphocyte culture probably reflects a response to both foreign histocompatibility determinants and determinants present on non-T lymphocytes. It is suggested that the proliferative response of T lymphocytes to autologous non-T lymphocytes may be a step in the process by which T lymphocytes regulate immunity.

Full Text

The Full Text of this article is available as a PDF (1,011.4 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Boyle W. An extension of the 51Cr-release assay for the estimation of mouse cytotoxins. Transplantation. 1968 Sep;6(6):761–764. doi: 10.1097/00007890-196809000-00002. [DOI] [PubMed] [Google Scholar]
  2. Chess L., MacDermott R. P., Schlossman S. F. Immunologic functions of isolated human lymphocyte subpopulations. II. Antigen triggering of T and B cells in vitro. J Immunol. 1974 Oct;113(4):1122–1127. [PubMed] [Google Scholar]
  3. Cohen I. R., Globerson A., Feldman M. Autosensitization in vitro. J Exp Med. 1971 Apr 1;133(4):834–845. doi: 10.1084/jem.133.4.834. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cohen I. R., Wekerle H. Autosensitization of lymphocytes against thymus reticulum cells. Science. 1972 Jun 23;176(4041):1324–1325. doi: 10.1126/science.176.4041.1324. [DOI] [PubMed] [Google Scholar]
  5. Cohen I. R., Wekerle H. Regulation of autosensitization. The immune activation and specific inhibition of self-recognizing thymus-derived lymphocytes. J Exp Med. 1973 Feb 1;137(2):224–238. doi: 10.1084/jem.137.2.224. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Cresswell P., Geler S. S. Antisera to human B-lymphocyte membrane glycoproteins block stimulation in mixed lymphocyte culture. Nature. 1975 Sep 11;257(5522):147–149. doi: 10.1038/257147a0. [DOI] [PubMed] [Google Scholar]
  7. Etheredge E. E., Shons A. R., Hohenthaner K. L., Najarian J. S. Mitomycin C inactivation of leukocytes in the mixed leukocyte culture. Transplantation. 1973 Mar;15(3):331–334. doi: 10.1097/00007890-197303000-00012. [DOI] [PubMed] [Google Scholar]
  8. Fridman W. H., Kourilsky F. M. Stimulation of lymphocytes by autologous leukaemic cells in acute leukaemia. Nature. 1969 Oct 18;224(5216):277–279. doi: 10.1038/224277a0. [DOI] [PubMed] [Google Scholar]
  9. Froland S. S., Natvig J. B. Identification of three different human lymphocyte populations by surface markers. Transplant Rev. 1973;16:114–162. doi: 10.1111/j.1600-065x.1973.tb00119.x. [DOI] [PubMed] [Google Scholar]
  10. Froland S. S., Wisloff F., Michaelsen T. E. Human lymphocytes with receptors for IgG. A population of cells distinct from T- and B-lymphocytes. Int Arch Allergy Appl Immunol. 1974;47(1):124–138. doi: 10.1159/000231207. [DOI] [PubMed] [Google Scholar]
  11. Gupta S., Grieco M. H. Rosette formation with mouse erythrocytes: probable marker for human B lymphocytes. Int Arch Allergy Appl Immunol. 1975;49(6):734–742. doi: 10.1159/000231457. [DOI] [PubMed] [Google Scholar]
  12. Harrison M. R., Paul W. E. Stimulus-response in the mixed lymphocyte reaction. J Exp Med. 1973 Dec 1;138(6):1602–1607. doi: 10.1084/jem.138.6.1602. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Hartzman R. J., Segall M., Bach M. L., Bach F. H. Histocompatibility matching. VI. Miniaturization of the mixed leukocyte culture test: a preliminary report. Transplantation. 1971 Mar;11(3):268–273. doi: 10.1097/00007890-197103000-00005. [DOI] [PubMed] [Google Scholar]
  14. Howe M. L., Goldstein A. L., Battisto J. R. Isogeneic lymphocyte interaction: recognition of self antigens by cells of the neonatal thymus. Proc Natl Acad Sci U S A. 1970 Oct;67(2):613–619. doi: 10.1073/pnas.67.2.613. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Ling N. R. Immune surveillance of lymphoid tissue. A biological role for the mixed lymphocyte reaction. Immunol Commun. 1973;2(2):119–127. doi: 10.3109/08820137309022785. [DOI] [PubMed] [Google Scholar]
  16. Llacer V., Uyeki E. M. Interaction of spleen and thymic cells in vitro. Int Arch Allergy Appl Immunol. 1969;35(1):88–96. doi: 10.1159/000230162. [DOI] [PubMed] [Google Scholar]
  17. Lohrmann H. P., Novikovs L., Graw R. G., Jr Stimulatory capacity of human T and B lymphocytes in the mixed leukocyte culture. Nature. 1974 Jul 12;250(462):144–146. doi: 10.1038/250144a0. [DOI] [PubMed] [Google Scholar]
  18. Lohrmann H. P., Whang-Peng J. Human mixed leukocyte culture: identification of the proliferating lymphocyte subpopulation by sex chromosome markers. J Exp Med. 1974 Jul 1;140(1):54–60. doi: 10.1084/jem.140.1.54. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Opelz G., Kiuchi M., Takasugi M., Terasaki P. I. Autologous stimulation of human lymphocyte subpopulation. J Exp Med. 1975 Nov 1;142(5):1327–1333. doi: 10.1084/jem.142.5.1327. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Perkins W. D., Robson L. C., Schwarz M. R. Immunoglobulin-positive cells: their appearance in the mixed lymphocyte reaction. Science. 1975 Apr 25;188(4186):365–366. doi: 10.1126/science.123355. [DOI] [PubMed] [Google Scholar]
  21. Piguet P. F., Vassalli P. Thymus-independent (B) cell proliferation in spleen cell cultures of mouse radiation chimeras stimulated by phytohemagglutinin or allogeneic cells. J Exp Med. 1972 Oct 1;136(4):962–967. doi: 10.1084/jem.136.4.962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Ponzio N. M., Finke J. H., Battisto J. R. Adult murine lymph node cells respond blastogenically to a new differentiation antigen on isologous and autologous B lymphocytes. J Immunol. 1975 Mar;114(3):971–975. [PubMed] [Google Scholar]
  23. Schwartz R. S. Another look at immunologic surveillance. N Engl J Med. 1975 Jul 24;293(4):181–184. doi: 10.1056/NEJM197507242930406. [DOI] [PubMed] [Google Scholar]
  24. Steel C. M., Evans J., Smith M. A. The sheep-cell rosette: test on human peripheral blood lymyphocytes: an analysis of some analysis of some variable factors in the technique. Br J Haematol. 1974 Oct;28(2):245–251. doi: 10.1111/j.1365-2141.1974.tb06659.x. [DOI] [PubMed] [Google Scholar]
  25. Von Boehmer H., Adams P. B. Syngeneic mixed lymphocyte reaction between thymocytes and peripheral lymphoid cells in mice: strain specificity and nature of the target cell. J Immunol. 1973 Feb;110(2):376–383. [PubMed] [Google Scholar]
  26. Von Boehmer H. Selective stimulation by B lymphocytes in the syngeneic mixed lymphocyte reaction. Eur J Immunol. 1974 Feb;4(2):105–110. doi: 10.1002/eji.1830040209. [DOI] [PubMed] [Google Scholar]
  27. Weksler M. E., Birnbasum G. Lymphocyte transformation induced by autologous cells: stimulation by cultured lymphoblast lines. J Clin Invest. 1972 Dec;51(12):3124–3132. doi: 10.1172/JCI107139. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Weksler M. E. Lymphocyte transformation induced by autologous cells. II. Stimulation by mitogen-induced lymphoblasts. J Exp Med. 1973 Mar 1;137(3):799–806. doi: 10.1084/jem.137.3.799. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Weksler M. E. Lymphocyte transformation induced by autologous cells. III. Lymphoblast-induced lymphocyte to stimulation does not correlate with EB viral antigen expression or immunity. J Immunol. 1976 Feb;116(2):310–314. [PubMed] [Google Scholar]
  30. Whitacre C., Lang R. W. Characterization of IgG-containing DEAE fractions of canine serum. Transplant Proc. 1975 Dec;7(4):531–536. [PubMed] [Google Scholar]
  31. Winchester R. J., Wernet P., Kunkel H. G., Dupont B., Jersild C., Fu S. M. Recognition by pregnancy serums of non-HL-A alloantigens selectively expressed on B lymphocytes. J Exp Med. 1975 Apr 1;141(4):924–929. [PMC free article] [PubMed] [Google Scholar]

Articles from The Journal of Experimental Medicine are provided here courtesy of The Rockefeller University Press

RESOURCES