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. 1976 May 1;143(5):1140–1153. doi: 10.1084/jem.143.5.1140

Alpha-fetoprotein synthesis by murine lymphoid cells in allogeneic reactions

PMCID: PMC2190199  PMID: 57206

Abstract

Surface-bound alpha-fetoprotein (AFP) was demonstrated by immunofluorescence on approximately 1/3 of splenic lymphocytes in chronic murine graft vs. host (GVH) reactions. Splenic lymphocytes were also shown to have a suppressed phytohemagglutinin (PHA) response compared to controls while lymph node cells from the same GVH animals revealed no surface AFP and had normal PHA responses. Splenic lymphocytes showed marked synthesis of AFP in the GVH and mixed lymphocyte culture (MLC) reactions by [14C]leucine incorporation and by radioimmunoassay in MLC supernates. Lymph node cells, however, demonstrated less synthetic activity and 14C counts in their membrane fractions were not markedly elevated.

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Selected References

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  1. Alexander P. Foetal "antigens" in cancer. Nature. 1972 Jan 21;235(5334):137–140. doi: 10.1038/235137a0. [DOI] [PubMed] [Google Scholar]
  2. Dattwyler R. J., Murgita R. A., Tomasi T. B., Jr Binding of alpha-foetoprotein to murine T cells. Nature. 1975 Aug 21;256(5519):656–657. doi: 10.1038/256656a0. [DOI] [PubMed] [Google Scholar]
  3. Dattwyler R. J., Tomasi T. B. Inhibition of sensitization of T-cells by alpha-fetoprotein. Int J Cancer. 1975 Dec 15;16(6):942–945. doi: 10.1002/ijc.2910160608. [DOI] [PubMed] [Google Scholar]
  4. Gleich G. J., Averbeck A. K., Swedlund H. A. Measurement of IgE in normal and allergic serum by radioimmunoassay. J Lab Clin Med. 1971 Apr;77(4):690–698. [PubMed] [Google Scholar]
  5. Hardin J. A., Chused T. M., Steinberg A. D. Supressor cells in the graft vs host reaction. J Immunol. 1973 Aug;111(2):650–651. [PubMed] [Google Scholar]
  6. Haurani F. I., Meyer A., O'Brien R. Production of transferrin by the macrophage. J Reticuloendothel Soc. 1973 Sep;14(3):309–316. [PubMed] [Google Scholar]
  7. Hirsch M. S., Phillips S. M., Solnik C., Black P. H., Schwartz R. S., Carpenter C. B. Activation of leukemia viruses by graft-versus-host and mixed lymphocyte reactions in vitro. Proc Natl Acad Sci U S A. 1972 May;69(5):1069–1072. doi: 10.1073/pnas.69.5.1069. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Häyry P., Andersson L. C., Nordling S., Virolainen M. Allograft response in vitro. Transplant Rev. 1972;12:91–140. doi: 10.1111/j.1600-065x.1972.tb00054.x. [DOI] [PubMed] [Google Scholar]
  9. Häyry P., Defendi V. I. Cultivation conditions and mixed lymphocyte interaction of mouse peripheral lymphocytes. Clin Exp Immunol. 1970 Mar;6(3):345–361. [PMC free article] [PubMed] [Google Scholar]
  10. Katz D. H., Ellman L., Paul W. E., Green I., Benacerraf B. Resistance of guinea pigs to leukemia following transfer of immunocompetent allogeneic lymphoid cells. Cancer Res. 1972 Jan;32(1):133–140. [PubMed] [Google Scholar]
  11. Kay J. E., Ahern T., Lindsay V. J., Sampson J. The control of protein synthesis during the stimulation of lymphocytes by phytohaemagglutinin. III. Poly(U) translation and the rate of polypeptide chain elongation. Biochim Biophys Acta. 1975 Jan 20;378(2):241–250. doi: 10.1016/0005-2787(75)90112-4. [DOI] [PubMed] [Google Scholar]
  12. Lagrange P. H., Mackaness G. B., Miller T. E. Potentiation of T-cell-mediated immunity by selective suppression of antibody formation with cyclophosphamide. J Exp Med. 1974 Jun 1;139(6):1529–1539. doi: 10.1084/jem.139.6.1529. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Lapp W. S., Möller G. Prolonged survival of H-2 incompatible skin allografts on F1 animals treated with parental lymphoid cells. Immunology. 1969 Sep;17(3):339–344. [PMC free article] [PubMed] [Google Scholar]
  14. MCELREE H., DOWNS C. M. The phagocytosis of Pasteurella tularensis by rat mononuclear cells as influenced by normal serums and various irritants. J Infect Dis. 1961 Jul-Aug;109:98–106. doi: 10.1093/infdis/109.1.98. [DOI] [PubMed] [Google Scholar]
  15. Melief C. J., Datta S., Louie S., Schwartz R. S. Immunologic activation of murine leukemia viruses. Cancer. 1974 Oct;34(4 Suppl):suppl–suppl:1487. doi: 10.1002/1097-0142(197410)34:8+<1481::aid-cncr2820340821>3.0.co;2-#. [DOI] [PubMed] [Google Scholar]
  16. Murgita R. A., Tomasi T. B., Jr Suppression of the immune response by alpha-fetoprotein on the primary and secondary antibody response. J Exp Med. 1975 Feb 1;141(2):269–286. doi: 10.1084/jem.141.2.269. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Möller G. Suppressive effect of graft versus host reactions on the immune response to heterologous red cells. Immunology. 1971 Apr;20(4):597–609. [PMC free article] [PubMed] [Google Scholar]
  18. Okubo S., Oettgen H. F., Caiazza S. S., Ovary Z. Enhancement of tumor growth by graft-versus-host reaction. Proc Natl Acad Sci U S A. 1974 Oct;71(10):4264–4266. doi: 10.1073/pnas.71.10.4264. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Osborne D. P., Jr, Katz D. H. The allogeneic effect in inbred mice. I. Experimental conditions for the enhancement of hapten-specific secondary antibody responses by the graft-versus-host reaction. J Exp Med. 1972 Sep 1;136(3):439–454. doi: 10.1084/jem.136.3.439. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. PREHN R. T., MAIN J. M. Immunity to methylcholanthrene-induced sarcomas. J Natl Cancer Inst. 1957 Jun;18(6):769–778. [PubMed] [Google Scholar]
  21. Peck A. B., Bach F. H. A miniaturized mouse mixed leukocyte culture in serum-free and mouse serum supplemented media. J Immunol Methods. 1973 Oct;3(2):147–163. doi: 10.1016/0022-1759(73)90030-6. [DOI] [PubMed] [Google Scholar]
  22. Phillips S. M., Gleichmann H., Hirsch M. S., Black P., Merrill J. P., Schwartz R. S., Carpenter C. B. Cellular immunity in the mouse. IV. Altered thymic-dependent lymphocyte reactivity in the chronic graft vs host reaction and leukemia virus activation. Cell Immunol. 1975 Jan;15(1):152–168. doi: 10.1016/0008-8749(75)90172-0. [DOI] [PubMed] [Google Scholar]
  23. Phillips S. M. Immunologic activation of oncogenic viruses. Prog Exp Tumor Res. 1974;19:37–44. doi: 10.1159/000395847. [DOI] [PubMed] [Google Scholar]
  24. Sjöberg O., Andersson J., Möller G. Reconstitution of the antibody response in vitro of T cell-deprived spleen cells by supernatants from spleen cell cultures. J Immunol. 1972 Dec;109(6):1379–1385. [PubMed] [Google Scholar]
  25. Tigelaar R. E., Asofsky R. Synergy among lymphoid cells mediating the graft-versus-host response. V. Derivation by migration in lethally irradiated recipients of two interacting subpopulations of thymus-derived cells from normal spleen. J Exp Med. 1973 Feb 1;137(2):239–253. doi: 10.1084/jem.137.2.239. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Weber K., Osborn M. The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis. J Biol Chem. 1969 Aug 25;244(16):4406–4412. [PubMed] [Google Scholar]

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