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. 1976 Jul 1;144(1):145–154. doi: 10.1084/jem.144.1.145

Immunologic characterization of the membrane-bound collagen in normal human fibroblasts: identification of a distinct membrane collagen

PMCID: PMC2190352  PMID: 932639

Abstract

Collagen, the major extracellular matrix protein, is also a membrane protein. Two types of collagen are detected on the normal human fibroblast membrane in culture, type I collagen and a new immunologically and chemically distinct collagen, type M (membrane) collagen. Antibodies to type M collagen elicited complement-mediated cytotoxicity, which could be blocked by pretreatment of the cells with bacterial collagenase or the antibody with type M collagen. Pretreatment of the cells with other proteolytic enzymes or the antibody with type I collagen or type III collagen had no effect on this complement-mediated cytotoxicity. Although type I collagen is the major collagen synthesized by normal human fibroblasts type M collagen may be the major cell membrane collagen and may be a major cell membrane component.

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Selected References

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