Skip to main content
PMC home page
The Journal of Experimental Medicine logoLink to The Journal of Experimental Medicine
. 1976 Jul 1;144(1):98–112. doi: 10.1084/jem.144.1.98

Isolation and immunologic characterization of a human. B-lymphocyte- specific, cell surface antigen

PMCID: PMC2190359  PMID: 58959

Abstract

In addition to HL-A antigens, another cell surface protein complex has been obtained from membranes of the human B-lymphoblast cell line IM-1. This complex which was solubilized with papain, consisted of polypeptides of 23,000 and 30,000 daltons (p23, 30). Rabbit antisera to this material precipitated from [35S]methionine-labeled detergent- solubilized cells, three proteins of 39,000, 34,000, and 29,000 daltons. These antisera were specifically cytotoxic for B lymphocytes of peripheral blood, for B-lymphoblast cell lines, and for EAC rosette receptor-positive surface Ig-negative (Null) lymphocytes. The p23,30 complex was not present on T lymphocytes, EAC rosette receptor-negative Null lymphocytes, or platelets. In addition, the p23,30 complex from several cell lines inhibited alloantisera from multiparous Amish women which had been shown to recognize non-HL-A, B-lymphocyte antigens. Some other properties of the anti-p23,30 sera antisera were described.

Full Text

The Full Text of this article is available as a PDF (1.2 MB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adams R. A., Flowers A., Davis B. J. Direct implantation and serial transplantation of human acute lymphoblastic leukemia in hamsters, SB-2. Cancer Res. 1968 Jun;28(6):1121–1125. [PubMed] [Google Scholar]
  2. Arbeit R. D., Sachs D. H., Amos D. B., Dickler H. B. Human lymphocyte alloantigen(s) similar to murine Ir region-associated (Ia) antigens. J Immunol. 1975 Oct;115(4):1173–1175. [PubMed] [Google Scholar]
  3. Chess L., Evans R., Humphreys R. E., Strominger J. L., Schlossman S. F. Inhibition of antibody-dependent cellular cytotoxicity and immunoglobulin synthesis by an antiserum prepared against a human B-cell Ia-like molecule. J Exp Med. 1976 Jul 1;144(1):113–122. doi: 10.1084/jem.144.1.113. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Chess L., MacDermott R. P., Schlossman S. F. Immunologic functions of isolated human lymphocyte subpopulations. I. Quantitative isolation of human T and B cells and response to mitogens. J Immunol. 1974 Oct;113(4):1113–1121. [PubMed] [Google Scholar]
  5. Crawford L. V., Gesteland R. F. Synthesis of polyoma proteins in vitro. J Mol Biol. 1973 Mar 15;74(4):627–634. doi: 10.1016/0022-2836(73)90053-3. [DOI] [PubMed] [Google Scholar]
  6. Cresswell P., Geler S. S. Antisera to human B-lymphocyte membrane glycoproteins block stimulation in mixed lymphocyte culture. Nature. 1975 Sep 11;257(5522):147–149. doi: 10.1038/257147a0. [DOI] [PubMed] [Google Scholar]
  7. Cullen S. E., David C. S., Shreffler D. C., Nathenson S. G. Membrane molecules determined by the H-2 associated immune response region: isolation and some properties. Proc Natl Acad Sci U S A. 1974 Mar;71(3):648–652. doi: 10.1073/pnas.71.3.648. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. David C. S., Cullen S. E., Murphy D. B. Serologic and biochemical studies of the Ia system of the mouse H-2 gene complex. Further evidence for I-C subregion. J Immunol. 1975 Apr;114(4):1205–1209. [PubMed] [Google Scholar]
  9. Fu S. M., Winchester R. J., Kunkel H. G. The occurrence of the HL-B alloantigens on the cells of unclassified acute lymphoblastic leukemias. J Exp Med. 1975 Nov 1;142(5):1334–1338. doi: 10.1084/jem.142.5.1334. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. GOODMAN H. S. A general method for the quantitation of immune cyotysis. Nature. 1961 Apr 15;190:269–270. doi: 10.1038/190269a0. [DOI] [PubMed] [Google Scholar]
  11. HUNTER W. M., GREENWOOD F. C. Preparation of iodine-131 labelled human growth hormone of high specific activity. Nature. 1962 May 5;194:495–496. doi: 10.1038/194495a0. [DOI] [PubMed] [Google Scholar]
  12. Hartzman R. J., Segall M., Bach M. L., Bach F. H. Histocompatibility matching. VI. Miniaturization of the mixed leukocyte culture test: a preliminary report. Transplantation. 1971 Mar;11(3):268–273. doi: 10.1097/00007890-197103000-00005. [DOI] [PubMed] [Google Scholar]
  13. Kekwick R. A. The serum proteins in multiple myelomatosis. Biochem J. 1940 Sep;34(8-9):1248–1257. doi: 10.1042/bj0341248. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Mac Dermott R. P., Chess L., Schlossman S. F. Immunologic functions of isolated human lymphocyte subpopulations. V. Isolation and functional analysis of a surface Ig negative, E rosette negative subset. Clin Immunol Immunopathol. 1975 Sep;4(3):415–424. doi: 10.1016/0090-1229(75)90010-0. [DOI] [PubMed] [Google Scholar]
  15. Mann D. L., Abelson L., Harris S., Amos D. B. Detection of antigens specific for B-lymphoid cultured cell lines with human alloantisera. J Exp Med. 1975 Jul 1;142(1):84–89. doi: 10.1084/jem.142.1.84. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. McCune J. M., Humphreys R. E., Yocum R. R., Strominger J. L. Enhanced representation of HL-A antigens on human lymphocytes after mitogenesis induced by phytohemagglutinin or Epstein-Barr virus. Proc Natl Acad Sci U S A. 1975 Aug;72(8):3206–3209. doi: 10.1073/pnas.72.8.3206. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. SANDERSON A. R. APPLICATIONS OF ISO-IMMUNE CYTOLYSIS USING RADIOLABELLED TARGET CELLS. Nature. 1964 Oct 17;204:250–253. doi: 10.1038/204250a0. [DOI] [PubMed] [Google Scholar]
  18. Sachs D. H., Cullen S. E., David C. S. Ir-associated murine alloantigens. Serological and chemical definition of Ia specificities associated with the H-2-b haplotype. Transplantation. 1975 May;19(5):388–393. [PubMed] [Google Scholar]
  19. Schlossman S. F., Chess L., Humphreys R. E., Strominger J. L. Distribution of Ia-like molecules on the surface of normal and leukemic human cells. Proc Natl Acad Sci U S A. 1976 Apr;73(4):1288–1292. doi: 10.1073/pnas.73.4.1288. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Tanigaki N., Miyakawa Y., Yagi Y., Pressman D. HL-A antigens from hematopoietic cell lines: molecular size and electrophoretic mobility. J Immunol. 1971 Aug;107(2):402–408. [PubMed] [Google Scholar]
  21. Turner M. J., Cresswell P., Parham P., Strominger J. L., Mann D. L., Sanderson A. R. Purification of papain-solubilized histocompatibility antigens from a cultured human lymphoblastoid line, RPMI 4265. J Biol Chem. 1975 Jun 25;250(12):4512–4519. [PubMed] [Google Scholar]
  22. WIGZELL H. QUANTITATIVE TITRATIONS OF MOUSE H-2 ANTIBODIES USING CR-51-LABELLED TARGET CELLS. Transplantation. 1965 May;3:423–431. doi: 10.1097/00007890-196505000-00011. [DOI] [PubMed] [Google Scholar]
  23. Winchester R. J., Wernet P., Kunkel H. G., Dupont B., Jersild C., Fu S. M. Recognition by pregnancy serums of non-HL-A alloantigens selectively expressed on B lymphocytes. J Exp Med. 1975 Apr 1;141(4):924–929. [PMC free article] [PubMed] [Google Scholar]

Articles from The Journal of Experimental Medicine are provided here courtesy of The Rockefeller University Press

RESOURCES