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. 1976 Oct 1;144(4):1141–1146. doi: 10.1084/jem.144.4.1141

Effects of anti-Ia sera on mitogenic responses. III. Mapping the genes controlling the expression of Ia determinants on concanavalin A- reactive cells to the I-J subregion of the H-2 gene complex

PMCID: PMC2190424  PMID: 62014

Abstract

We have shown that the Ia determinants expressed on nylon wool-purified T lymphocytes reactive to concanavalin A (Con A) in serum-free media are coded in a single I subregion of the H-2 gene complex. This region, I-J, is defined by two pairs of intra-H-2 recombinant haplotypes: H- 2t3, H-2t4 and H-2i3, H-2i5, carried by B10.HTT, B10.S(9R), B10.A(3R), AND B10.A(5R), respectively. No activity against Con A-reactive T cells has been detected in any antiserum that was produced in strain combinations which shared a common I-J region. This suggests that Ia antigens expressed on Con A-reactive T cells are restricted to the I-J subregion.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. David C., Meo T., McCormick J., Shreffler D. Expression of individual Ia specificities on T and B cells. I. Studies with mitogen-induced blast cells. J Exp Med. 1976 Jan 1;143(1):218–224. doi: 10.1084/jem.143.1.218. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Fathman C. G., Cone J. L., Sharrow S. O., Tyrer H., Sachs D. H. Ia alloantigen(s) detected on thymocytes by use of a fluorescence-activated cell sorter. J Immunol. 1975 Aug;115(2):584–589. [PubMed] [Google Scholar]
  3. Frelinger J. A., Neiderhuber J. E., David C. S., Shreffler D. C. Evidence for the expression of Ia (H-2-associated) antigens on thymus-derived lymphocytes. J Exp Med. 1974 Nov 1;140(5):1273–1284. doi: 10.1084/jem.140.5.1273. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hauptfeld V., Hauptfeld M., Klein J. Tissue distribution of I region-associated antigens in the mouse. J Immunol. 1974 Jul;113(1):181–188. [PubMed] [Google Scholar]
  5. Hirst J. A., Beverley P. C., Kisielow P., Hoffmann M. K., Oettgen H. F. Ly antigens: markers of T cell function on mouse spleen cells. J Immunol. 1975 Dec;115(6):1555–1557. [PubMed] [Google Scholar]
  6. Jandinski J., Cantor H., Tadakuma T., Peavy D. L., Pierce C. W. Separation of helper T cells from suppressor T cells expressing different Ly components. I. Polyclonal activation: suppressor and helper activities are inherent properties of distinct T-cell subclasses. J Exp Med. 1976 Jun 1;143(6):1382–1390. doi: 10.1084/jem.143.6.1382. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Julius M. H., Simpson E., Herzenberg L. A. A rapid method for the isolation of functional thymus-derived murine lymphocytes. Eur J Immunol. 1973 Oct;3(10):645–649. doi: 10.1002/eji.1830031011. [DOI] [PubMed] [Google Scholar]
  8. Niederhuber J. E., Frelinger J. A., Dine M. S., Shoffner P., Dugan E., Shreffler D. C. Effects of anti-Ia sera on mitogenic responses. II. Differential expression of the Ia marker on phytohemagglutinin and concanavalin A-reactive T cells. J Exp Med. 1976 Feb 1;143(2):372–381. doi: 10.1084/jem.143.2.372. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Niederhuber J. E., Frelinger J. A., Dugan E., Coutinho A., Shreffler D. C. Effects of anti-Ia serum on mitogenic responses. I. Inhibition of the proliferative response to B cell mitogen, LPS, by specific anti-Ia sera. J Immunol. 1975 Dec;115(6):1672–1676. [PubMed] [Google Scholar]
  10. Niederhuber J. E., Frelinger J. A. Expression of Ia antigens on T and B cells and their relationship to immune-response functions. Transplant Rev. 1976;30:101–121. [PubMed] [Google Scholar]
  11. Plate J. M. Cellular responses to murine alloantigens of the major histocompatibility complex: the role of cell subpopulations that express different quantities of H-2 associated antigenic markers. Eur J Immunol. 1976 Mar;6(3):180–187. doi: 10.1002/eji.1830060308. [DOI] [PubMed] [Google Scholar]
  12. Sachs D. H., Cone J. L. A mouse B-cell alloantigen determined by gene(s) linked to the major histocompatibility complex. J Exp Med. 1973 Dec 1;138(6):1289–1304. doi: 10.1084/jem.138.6.1289. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Shreffler D. C., David C. S. The H-2 major histocompatibility complex and the I immune response region: genetic variation, function, and organization. Adv Immunol. 1975;20:125–195. doi: 10.1016/s0065-2776(08)60208-4. [DOI] [PubMed] [Google Scholar]
  14. Stout R. D., Herzenberg L. A. The Fc receptor on thymus-derived lymphocytes: II. Mitogen responsiveness of T lymphocytes bearing the Fc receptor. J Exp Med. 1975 Nov 1;142(5):1041–1051. doi: 10.1084/jem.142.5.1041. [DOI] [PMC free article] [PubMed] [Google Scholar]

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