Skip to main content
NCBI home page
The Journal of Experimental Medicine logoLink to The Journal of Experimental Medicine
. 1976 Nov 2;144(5):1284–1293. doi: 10.1084/jem.144.5.1284

Isoantiserum-augmented development of lymphocyte-mediated cytotoxicity

PMCID: PMC2190458  PMID: 1086884

Abstract

Passive administration of anti-P-814 isoantiserum (IS) with P-815 mastocytoma was shown to augment the development of T-lymphocyte- mediated cytotoxicity (LMC). The LMC activity augmented by IS was specific to immunizing tumor cells, but required the simultaneous administration of P-815 tumor cells and anti-P-815 serum, suggesting that the antigen-antibody complex is involved in the development of specific cytotoxic T cells. The serum component responsible for augmented development of LMC activity appeared to be IgM in that the augmenting activity fractionated in the void volume of a G-200 Sephadex column and appears very early after immunization. Our experimental results suggest the development of specific T-cell cytotoxicity can be directly regulated by specific IgM antibodies.

Full Text

The Full Text of this article is available as a PDF (654.4 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baldwin R. W., Price M. R., Robins R. A. Blocking of lymphocyte-mediated cytotoxicity for rat hepatoma cells by tumour-specific antigen-antibody complexes. Nat New Biol. 1972 Aug 9;238(84):185–186. doi: 10.1038/newbio238185a0. [DOI] [PubMed] [Google Scholar]
  2. Berke G., Sullivan K. A., Amos B. Rejection of ascites tumor allografts. I. Isolation, characterization, and in vitro reactivity of peritoneal lymphoid effector cells from BALB-c mice immune to EL4 leukosis. J Exp Med. 1972 Jun 1;135(6):1334–1350. doi: 10.1084/jem.135.6.1334. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Boyse E. A., Hubbard L., Stockert E., Lamm M. E. Improved complementation in the cytotoxic test. Transplantation. 1970 Nov;10(5):446–449. doi: 10.1097/00007890-197011000-00019. [DOI] [PubMed] [Google Scholar]
  4. Canty T. G., Wunderlich J. R. Quantitative in vitro assay of cytotoxic cellular immunity. J Natl Cancer Inst. 1970 Oct;45(4):761–772. [PubMed] [Google Scholar]
  5. Cerottini J. C., Nordin A. A., Brunner K. T. Specific in vitro cytotoxicity of thymus-derived lymphocytes sensitized to alloantigens. Nature. 1970 Dec 26;228(5278):1308–1309. doi: 10.1038/2281308a0. [DOI] [PubMed] [Google Scholar]
  6. Cohen J. M., Yang S. S., Law L. W. Abrogation of cell-mediated immunity by hyperimmune alloantiserum: mechanisms and correlation with allograft enhancement. Int J Cancer. 1974 Apr 15;13(4):463–477. doi: 10.1002/ijc.2910130405. [DOI] [PubMed] [Google Scholar]
  7. Dennert G. The mechanism of antibody-induced stimulation and inhibition of the immune response. J Immunol. 1971 Apr;106(4):951–955. [PubMed] [Google Scholar]
  8. Faanes R. B., Choi Y. S., Good R. A. Escape from isoantiserum inhibition of lymphocyte-mediated cytotoxicity. J Exp Med. 1973 Jan 1;137(1):171–182. doi: 10.1084/jem.137.1.171. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Faanes R. B., Choi Y. S. Interaction of isoantibody and cytotoxic lymphocytes with allogeneic tumor cells. J Immunol. 1974 Jul;113(1):279–288. [PubMed] [Google Scholar]
  10. Gorczynski R. M., Kilburn D. G., Knight R. A., Norbury C., Parker D. C., Smith J. B. Nonspecific and specific immunosuppression in tumour-bearing mice by soluble immune complexes. Nature. 1975 Mar 13;254(5496):141–143. doi: 10.1038/254141a0. [DOI] [PubMed] [Google Scholar]
  11. Gordon J., Murgita R. A. Suppression and augmentation of the primary in vitro immune response by different classes of antibodies. Cell Immunol. 1975 Feb;15(2):392–402. doi: 10.1016/0008-8749(75)90017-9. [DOI] [PubMed] [Google Scholar]
  12. Greenberg A. H., Shen L. A class of specific cytotoxic cells demonstrated in vitro by arming with antigen-antibody complexes. Nat New Biol. 1973 Oct 31;245(148):282–285. doi: 10.1038/newbio245282a0. [DOI] [PubMed] [Google Scholar]
  13. Henry C., Jerne N. K. Competition of 19S and 7S antigen receptors in the regulation of the primary immune response. J Exp Med. 1968 Jul 1;128(1):133–152. doi: 10.1084/jem.128.1.133. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Hudson L., Sprent J. Specific adsorption of IgM antibody onto H-2-activated mouse T lymphocytes. J Exp Med. 1976 Feb 1;143(2):444–449. doi: 10.1084/jem.143.2.444. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Jansen J. L., Koene R. A., Kamp G. J., Hagemann J. F., Wijdeveld P. G. Hyperacute rejection and enhancement of mouse skin grafts by antibodies with a distinct specificity. J Immunol. 1975 Aug;115(2):392–394. [PubMed] [Google Scholar]
  16. Julius M. H., Simpson E., Herzenberg L. A. A rapid method for the isolation of functional thymus-derived murine lymphocytes. Eur J Immunol. 1973 Oct;3(10):645–649. doi: 10.1002/eji.1830031011. [DOI] [PubMed] [Google Scholar]
  17. Kaliss N. Immunological enhancement. Int Rev Exp Pathol. 1969;8:241–276. [PubMed] [Google Scholar]
  18. Kappler J. W., Hoffmann M., Dutton R. W. Regulation of the immune response. I. Differential effect of passively administered antibody on the thymus-derived and bone marrow-derived lymphocytes. J Exp Med. 1971 Sep 1;134(3 Pt 1):577–587. doi: 10.1084/jem.134.3.577. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Klein P. A. Anomalous reactions of mouse alloantisera with cultured tumor cells. I. Demonstration of widespread occurrence using reference typing sera. J Immunol. 1975 Nov;115(5):1254–1260. [PubMed] [Google Scholar]
  20. Liew F. Y., Parish C. R. Regulation of the immune response by antibody. I. Suppression of antibody formation and concomitant enhancement of cell-mediated immunity by passive antibody. Cell Immunol. 1972 May;4(1):66–85. doi: 10.1016/0008-8749(72)90006-8. [DOI] [PubMed] [Google Scholar]
  21. Lonai P., Clark W. R., Feldman M. Participation of theta-bearing cell in an in vitro assay of transplantation immunity. Nature. 1971 Feb 19;229(5286):566–567. doi: 10.1038/229566a0. [DOI] [PubMed] [Google Scholar]
  22. Lustig H. J., Bianco C. Antibody-mediated cell cytotoxicity in a defined system: regulation by antigen, antibody, and complement. J Immunol. 1976 Jan;116(1):253–260. [PubMed] [Google Scholar]
  23. Mackaness G. B., Lagrange P. H. Restoration of cell-mediated immunity to animals blocked by a humoral response. J Exp Med. 1974 Sep 1;140(3):865–870. doi: 10.1084/jem.140.3.865. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Mitchell M. S. Central inhibition of cellular immunity to leukemia L1210 by isoantibody. Cancer Res. 1972 Apr;32(4):825–831. [PubMed] [Google Scholar]
  25. Playfair J. H. The role of antibody in T-cell responses. Clin Exp Immunol. 1974 May;17(1):1–18. [PMC free article] [PubMed] [Google Scholar]
  26. Ptak W., Hanczakowska M. Alloantibody-induced cytotoxicity of macrophages. J Immunol. 1975 Sep;115(3):796–799. [PubMed] [Google Scholar]
  27. Shearer W. T., Philpott G. W., Parker C. W. Stimulation of cells by antibody. Science. 1973 Dec 28;182(4119):1357–1359. doi: 10.1126/science.182.4119.1357. [DOI] [PubMed] [Google Scholar]
  28. Shin H. S., Kaliss N., Borenstein D. Antibody-mediated suppression of grafted lymphoma cells. I. Participation of a host factor(s) other than complement. Proc Soc Exp Biol Med. 1972 Feb;139(2):684–689. doi: 10.3181/00379727-139-36216. [DOI] [PubMed] [Google Scholar]
  29. Takasugi M., Klein E. The role of blocking antibodies in immunological enhancement. Immunology. 1971 Oct;21(4):675–684. [PMC free article] [PubMed] [Google Scholar]
  30. Uhr J. W., Möller G. Regulatory effect of antibody on the immune response. Adv Immunol. 1968;8:81–127. doi: 10.1016/s0065-2776(08)60465-4. [DOI] [PubMed] [Google Scholar]
  31. Waksman B. H., Namba Y. On soluble mediators of immunologic regulation. Cell Immunol. 1976 Jan;21(1):161–176. doi: 10.1016/0008-8749(76)90337-3. [DOI] [PubMed] [Google Scholar]
  32. Zighelboim J., Bonavida B., Fahey J. L. Antibody-mediated in vivo suppression of EL4 leukemia in a syngeneic host. J Natl Cancer Inst. 1974 Mar;52(3):879–881. doi: 10.1093/jnci/52.3.879. [DOI] [PubMed] [Google Scholar]

Articles from The Journal of Experimental Medicine are provided here courtesy of The Rockefeller University Press

RESOURCES