Abstract
Human red blood cells sensitized with concanavalin A became bound to homologous peripheral blood monocytes. Binding occured at a concentration of 10(5) molecules of tetrameric Con A per red blood cell (RBC) and increased with additional Con A. RBC binding began within 5 min and was maximal at 90 min. Phagocytosis of sensitized RBCs was minimal. RBC attachment was prevented by 0.01 M alpha-methyl-D- mannopyranoside, and, once the RBC-monocyte rosette was established, bound RBCs were largely removed with this specific saccharide inhibitor of Con A. RBCs attached to monocytes became spherocytic and osmotically fragile. The recognition of concanavalin A (Con A)-coated RBCs was not mediated through the monocyte IgG-Fc receptor. These studies demonstrate that, like IgG and C3b, Con A is capable of mediating the binding of human RBCs to human monocytes. Red cells so bound are damaged at the monocyte surface.
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Selected References
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- Allen J. M., Cook G. M., Poole A. R. Action of concanavalin A on the attachment stage of phagocytosis by macrophages. Exp Cell Res. 1971 Oct;68(2):466–467. doi: 10.1016/0014-4827(71)90178-9. [DOI] [PubMed] [Google Scholar]
- Bianco C., Griffin F. M., Jr, Silverstein S. C. Studies of the macrophage complement receptor. Alteration of receptor function upon macrophage activation. J Exp Med. 1975 Jun 1;141(6):1278–1290. doi: 10.1084/jem.141.6.1278. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Borsos T., Colten H. R., Spalter J. S., Rogentine N., Rapp H. J. The C'la fixation and transfer test: examples of its applicability to the detection and enumeration of antigens and antibodies at cell surfaces. J Immunol. 1968 Sep;101(3):392–398. [PubMed] [Google Scholar]
- Goldman R., Cooper R. A. Concanavalin A mediated attachment and ingestion of red blood cells by macrophages. Exp Cell Res. 1975 Oct 1;95(1):223–231. doi: 10.1016/0014-4827(75)90627-8. [DOI] [PubMed] [Google Scholar]
- Griffin F. M., Jr, Silverstein S. C. Segmental response of the macrophage plasma membrane to a phagocytic stimulus. J Exp Med. 1974 Feb 1;139(2):323–336. doi: 10.1084/jem.139.2.323. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Huber H., Polley M. J., Linscott W. D., Fudenberg H. H., Müller-Eberhard H. J. Human monocytes: distinct receptor sites for the third component of complement and for immunoglobulin G. Science. 1968 Dec 13;162(3859):1281–1283. doi: 10.1126/science.162.3859.1281. [DOI] [PubMed] [Google Scholar]
- Inoue M., Mori M., Utsumi K., Seno S. Role of concanavalin-A in tumor cell agglutination and adhesion to macrophages. Gan. 1972 Dec;63(6):795–799. [PubMed] [Google Scholar]
- Jones T. C., Hirsch J. G. The interaction in vitro of Mycoplasma pulmonis with mouse peritoneal macrophages and L-cells. J Exp Med. 1971 Feb 1;133(2):231–259. doi: 10.1084/jem.133.2.231. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lay W. H., Nussenzweig V. Receptors for complement of leukocytes. J Exp Med. 1968 Nov 1;128(5):991–1009. doi: 10.1084/jem.128.5.991. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LoBuglio A. F., Cotran R. S., Jandl J. H. Red cells coated with immunoglobulin G: binding and sphering by mononuclear cells in man. Science. 1967 Dec 22;158(3808):1582–1585. doi: 10.1126/science.158.3808.1582. [DOI] [PubMed] [Google Scholar]
- MULLER-EBERHARD H. J., KUNKEL H. G. The carbohydrate of gamma-globulin and myeloma proteins. J Exp Med. 1956 Aug 1;104(2):253–269. doi: 10.1084/jem.104.2.253. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mantovani B., Rabinovitch M., Nussenzweig V. Phagocytosis of immune complexes by macrophages. Different roles of the macrophage receptor sites for complement (C3) and for immunoglobulin (IgG). J Exp Med. 1972 Apr 1;135(4):780–792. doi: 10.1084/jem.135.4.780. [DOI] [PMC free article] [PubMed] [Google Scholar]
- McConahey P. J., Dixon F. J. A method of trace iodination of proteins for immunologic studies. Int Arch Allergy Appl Immunol. 1966;29(2):185–189. doi: 10.1159/000229699. [DOI] [PubMed] [Google Scholar]
- Schreiber A. D., Parsons J., McDermott P., Cooper R. A. Effect of corticosteroids on the human monocyte IgG and complement receptors. J Clin Invest. 1975 Nov;56(5):1189–1197. doi: 10.1172/JCI108196. [DOI] [PMC free article] [PubMed] [Google Scholar]