Abstract
T lymphocytes from guinea pigs immunized with 2,4-dinitrophenyl (DNP) derivatives of mycobacteria respond to a variety of DNP conjugates. Preincubation of such cells with a given DNP conjugate under conditions which lead to the inactivation of responding cells causes a loss of the response to that conjugate, but has little effect on the response to DNP coupled to unrelated carriers. Thus, the responses of such cells to a variety of DNP conjugates can best be explained by the presence of a mixture of highly specific cells each responding to a different antigenic dterminant rather than by the presence of T cells with specificity limited to the hapten itself. Furthermore, the activity of T cells from DNP-mycobacteria-primed donors could not be blocked by a variety of nonstimulatory DNP conjugates. This suggests that while such T cells clearly recognize DNP with great precision, the receptor does not contain a very high affinity site for the hapten. A possible model for such a T-cell receptor is discussed.
Full Text
The Full Text of this article is available as a PDF (1.2 MB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Alkan S. S., El-Khateeb M. Comparison of specificities of humoral and cellular immune response to haptens. Eur J Immunol. 1975 Nov;5(11):766–770. doi: 10.1002/eji.1830051107. [DOI] [PubMed] [Google Scholar]
- Benacerraf B., Katz D. H. Failure to induce tolerance to 2,4-dinitrochlorobenzene contact sensitivity with a 2,4-dinitrophenyl (DNP) conjugate of a copolymer of D-glutamic acid and D-lysine, a specific tolerogen for DNP B cells. J Immunol. 1974 Mar;112(3):1158–1163. [PubMed] [Google Scholar]
- Cohen B. E., Davie J. M., Paul W. E. Hapten-specific tolerance: depressed humoral and normal cellular immune responses. J Immunol. 1973 Jan;110(1):213–218. [PubMed] [Google Scholar]
- Cohen B. E., Rosenthal A. S., Paul W. E. Antigen-macrophage interaction. I. Hapten-specific inhibition of antigen interaction with macrophages from immune animals. J Immunol. 1973 Sep;111(3):811–819. [PubMed] [Google Scholar]
- Davie J. M., Paul W. E., Katz D. H., Benacerraf B. Hapten-specific tolerance. Preferential depression of the high affinity antibody response. J Exp Med. 1972 Sep 1;136(3):426–438. doi: 10.1084/jem.136.3.426. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Doughty R. A., Klinman N. R. Carrier independent T cell helper effects in antigenic stimulation. J Immunol. 1973 Oct;111(4):1140–1146. [PubMed] [Google Scholar]
- GREENWOOD F. C., HUNTER W. M., GLOVER J. S. THE PREPARATION OF I-131-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY. Biochem J. 1963 Oct;89:114–123. doi: 10.1042/bj0890114. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Janeway C. A., Cohen B. E., Ben-Sasson S. Z., Paul W. E. The specificity of cellular immune responses in guinea pigs. I. T cells specific for 2,4-dinitrophenyl-o-tyrosyl residues. J Exp Med. 1975 Jan 1;141(1):42–55. doi: 10.1084/jem.141.1.42. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Janeway C. A., Jr, Humphrey J. H. Synthetic antigens composed exclusively of L- or D- amino acids. II. Effect of optical configuration on the metabolism and fate of synthetic polypeptide antigens in mice. Immunology. 1968 Feb;14(2):225–234. [PMC free article] [PubMed] [Google Scholar]
- Janeway C. A., Jr, Sela M. Synthetic antigens composed exclusively of L- or D-amino acids. I. Effect of optical configuration on the immunogenicity of synthetic polypeptides in mice. Immunology. 1967 Jul;13(1):29–38. [PMC free article] [PubMed] [Google Scholar]
- Janeway C. A., Jr The specificity of T lymphocyte responses to chemically defined antigens. Transplant Rev. 1976;29:164–188. doi: 10.1111/j.1600-065x.1976.tb00201.x. [DOI] [PubMed] [Google Scholar]
- Janeway C. A., Maurer P. H., Dailey M. O., Inman J. K. The specificity of cellular immune responses II. The structure of antigenic determinants leading to T-lymphocyte stimulation. J Exp Med. 1976 Dec 1;144(6):1621–1640. doi: 10.1084/jem.144.6.1621. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Paul W. E. Functional specificity of antigen-binding receptors of lymphocytes. Transplant Rev. 1970;5:130–166. doi: 10.1111/j.1600-065x.1970.tb00359.x. [DOI] [PubMed] [Google Scholar]
- Rich R. R., Kirkpatrick C. H., Smith T. K. Simultaneous suppression of responses to allogeneic tissue in vitro and in vivo. Cell Immunol. 1972 Sep;5(1):190–200. doi: 10.1016/0008-8749(72)90095-0. [DOI] [PubMed] [Google Scholar]
- Rosenstreich D. L., Blake J. T., Rosenthal A. S. The peritoneal exudate lymphocyte. I. Differences in antigen responsiveness between peritoneal exudate and lymph node lymphocytes from immunized guinea pigs. J Exp Med. 1971 Nov 1;134(5):1170–1186. doi: 10.1084/jem.134.5.1170. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rubin B., Wigzell H. Hapten-reactive helper lymphocytes. Nature. 1973 Apr 13;242(5398):467–469. doi: 10.1038/242467a0. [DOI] [PubMed] [Google Scholar]
- Stupp Y., Yoshida T., Paul W. E. Determination of antibody-hapten equilibrium constants by an ammonium sulfate precipitation technique. J Immunol. 1969 Sep;103(3):625–627. [PubMed] [Google Scholar]
- Taylor R. B., Iverson G. M. Hapten competition and the nature of cell-cooperation in the antibody response. Proc R Soc Lond B Biol Sci. 1971 Jan 12;176(1045):393–418. doi: 10.1098/rspb.1971.0003. [DOI] [PubMed] [Google Scholar]
- Zoschke D. C., Bach F. H. Specificity of antigen recognition by human lymphocytes in vitro. Science. 1970 Dec 25;170(3965):1404–1406. doi: 10.1126/science.170.3965.1404. [DOI] [PubMed] [Google Scholar]