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. 1976 Dec 1;144(6):1545–1553. doi: 10.1084/jem.144.6.1545

Specific binding of K- and I-region products of the H-2 complex to activated thymus-derived (T) cells belonging to different Ly subclasses

PMCID: PMC2190479  PMID: 137268

Abstract

Responder cells [C57BL/6J X A.TL)F1 lymph node cells depleted of bursa equivalent-derived (B) cells by filtration through nylon wool columns] were activated against incompatible K-region and I-region products together under conditions where these antigens are presented on separate stimulator cells. The resulting T blasts were stained with different concentrations of antisera directed against incompatible stimulator K-region or I-region products, or both. We obtained results that strongly suggest that in these cultures each activated responder blast stains with antiserum directed against either K-region or I- region products, but not both. Responder blasts from the same cultures were treated with antiserum and complement (C) directed against either Ly-1.2 or Ly-2.2 T-cell-specific surface antigens. Anti-Ly-1.2 serum and C specifically eliminates virtually all responder blasts staining with antiserum directed against stimulator I-region products; whereas anti-Ly-2.2 serum reduces to background levels the proportion of cells staining with antiserum against stimulator K-region products. The results obtained suggest that T cells binding stimulator K-region and I- region products, respectively, belong to two different subclasses distinguishable by their Ly phenotypes. Possible explanations for this association of T- cell subclass and specificity are discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bach F. H., Widmer M. B., Bach M. L., Klein J. Serologically defined and lymphocyte-defined components of the major histocompatibility complex in the mouse. J Exp Med. 1972 Dec 1;136(6):1430–1444. doi: 10.1084/jem.136.6.1430. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bach F. H., Widmer M. B., Segall M., Bach M. L., Klein J. Genetic and immunological complexity of major histocompatibility regions. Science. 1972 Jun 2;176(4038):1024–1027. doi: 10.1126/science.176.4038.1024. [DOI] [PubMed] [Google Scholar]
  3. Boyse E. A., Hubbard L., Stockert E., Lamm M. E. Improved complementation in the cytotoxic test. Transplantation. 1970 Nov;10(5):446–449. doi: 10.1097/00007890-197011000-00019. [DOI] [PubMed] [Google Scholar]
  4. Cantor H., Boyse E. A. Functional subclasses of T lymphocytes bearing different Ly antigens. II. Cooperation between subclasses of Ly+ cells in the generation of killer activity. J Exp Med. 1975 Jun 1;141(6):1390–1399. doi: 10.1084/jem.141.6.1390. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cantor H., Boyse E. A. Functional subclasses of T-lymphocytes bearing different Ly antigens. I. The generation of functionally distinct T-cell subclasses is a differentiative process independent of antigen. J Exp Med. 1975 Jun 1;141(6):1376–1389. doi: 10.1084/jem.141.6.1376. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. David C. S., Shreffler D. C. I region-associated antigen system (Ia) of the mouse H-2 gene complex. Further definition of Ia secificities with restricted anti-Ia antisera. Transplantation. 1974 Oct;18(4):313–321. doi: 10.1097/00007890-197410000-00003. [DOI] [PubMed] [Google Scholar]
  7. Hudson L., Sprent J. Specific adsorption of IgM antibody onto H-2-activated mouse T lymphocytes. J Exp Med. 1976 Feb 1;143(2):444–449. doi: 10.1084/jem.143.2.444. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Itakura K., Hutton J. J., Boyse E. A., Old L. J. Genetic linkage relationships of loci specifying differentiation alloantigens in the mouse. Transplantation. 1972 Mar;13(3):239–243. doi: 10.1097/00007890-197203000-00007. [DOI] [PubMed] [Google Scholar]
  9. Jerne N. K. The somatic generation of immune recognition. Eur J Immunol. 1971 Jan;1(1):1–9. doi: 10.1002/eji.1830010102. [DOI] [PubMed] [Google Scholar]
  10. Julius M. H., Simpson E., Herzenberg L. A. A rapid method for the isolation of functional thymus-derived murine lymphocytes. Eur J Immunol. 1973 Oct;3(10):645–649. doi: 10.1002/eji.1830031011. [DOI] [PubMed] [Google Scholar]
  11. Meo T., Vives G., Rijnbeek A. M., Miggiano V. C., Nabholz M., Shreffler D. C. A bipartite interpretation and tentative mapping of H-2-associated MLR determinants in the mouse. Transplant Proc. 1973 Dec;5(4):1339–1350. [PubMed] [Google Scholar]
  12. Miggiano V. C., Meo T., Bergén I., Nabholz M. Further miniaturization and automation of in vitro lymphocyte cultures. Tissue Antigens. 1975 Apr;5(3):173–185. doi: 10.1111/j.1399-0039.1975.tb01212.x. [DOI] [PubMed] [Google Scholar]
  13. Nabholz M., Vives J., Young H. M., Meo T., Miggiano V., Rijnbeek A., Shreffler D. C. Cell-mediated cell lysis in vitro: genetic control of killer cell production and target specificities in the mouse. Eur J Immunol. 1974 May;4(5):378–387. doi: 10.1002/eji.1830040514. [DOI] [PubMed] [Google Scholar]
  14. Nagy Z., Elliott B. E., Nabholz M., Krammer P. H., Pernis B. Specific binding of alloantigens to T cells activated in the mixed lymphocyte reaction. J Exp Med. 1976 Mar 1;143(3):648–659. doi: 10.1084/jem.143.3.648. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Sauser D., Anckers C., Bron C. Isolation of mouse thymus-derived lymphocyte specific surface antigens. J Immunol. 1974 Aug;113(2):617–624. [PubMed] [Google Scholar]

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