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. 1975 Feb 1;141(2):483–496. doi: 10.1084/jem.141.2.483

Inhibition of multiplication of Toxoplasma gondii by human monocytes exposed to T-lymphocyte products

PMCID: PMC2190524  PMID: 1089746

Abstract

The multiplication of Toxoplasma gondii was quantitated in human monocytes in vitro by phase-contrast microscopy. Toxoplasma multiplication was identical in monocytes from subjects byt was significantly inhibited in cells from both sources if the monocytes were preincubated with immune lymphocytes and toxoplasma monocytes were preincubated with immune lymphocytes and toxoplasma antigen. Supernates prepared from toxoplasma-immune lymphocytes incubated with toxoplasma antigen were also effective in inducing in monocytes the capacity to inhibit toxoplasma multiplication. Supernative acitivty was evident after lymphocytes and antigen were incubated for as little as 15 min. The instruction of monocytes was also repid and reversible. Monocytes were fully induced to inhibit toxoplasma multiplication after a 2 h exposure to an active supernate, but they lost their inhibitory capacity on culture in vitro for 48 h in the absece of immune cells or their products. The lymphocytes particupating in the monocyte induction were identified as t cells. The in vitro stimulation of monocytes appeared to exhibit some specificity, since no inhibition of toxopreotein derivative and lymphocytes from tuberculin-positive subjects, concanavalin a-stimulated lymphocytes, or their supermates. Supernates which induced monocytes to inhibit toxoplasma multiplication did not influence parasite growth in HeLa cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson S. E., Jr, Remington J. S. Effect of normal and activated human macrophages on Toxoplasma gondii. J Exp Med. 1974 May 1;139(5):1154–1174. doi: 10.1084/jem.139.5.1154. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Blanden R. V., Langman R. E. Cell-mediated immunity to bacterial infection in the mouse. Thymus-derived cells as effectors of acquired resistance to Listeria monocytogenes. Scand J Immunol. 1972;1(4):379–391. doi: 10.1111/j.1365-3083.1972.tb03304.x. [DOI] [PubMed] [Google Scholar]
  3. Blanden R. V. Mechanisms of recovery from a generalized viral infection: mousepox. II. Passive transfer of recovery mechanisms with immune lymphoid cells. J Exp Med. 1971 May 1;133(5):1074–1089. doi: 10.1084/jem.133.5.1074. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Desmonts G., Couvreur J. Congenital toxoplasmosis. A prospective study of 378 pregnancies. N Engl J Med. 1974 May 16;290(20):1110–1116. doi: 10.1056/NEJM197405162902003. [DOI] [PubMed] [Google Scholar]
  5. Fowles R. E., Fajardo I. M., Leibowitch J. L., David J. R. The enhancement of macrophage bacteriostasis by products of activated lymphocytes. J Exp Med. 1973 Oct 1;138(4):952–964. doi: 10.1084/jem.138.4.952. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Frenkel J. K. Adoptive immunity to intracellular infection. J Immunol. 1967 Jun;98(6):1309–1319. [PubMed] [Google Scholar]
  7. Godal T., Rees R. J., Lamvik J. O. Lymphocyte-mediated modification of blood-derived macrophage function in vitro; inhibition of growth of intracellular mycobacteria with lymphokines. Clin Exp Immunol. 1971 Apr;8(4):625–637. [PMC free article] [PubMed] [Google Scholar]
  8. Gordon S., Todd J., Cohn Z. A. In vitro synthesis and secretion of lysozyme by mononuclear phagocytes. J Exp Med. 1974 May 1;139(5):1228–1248. doi: 10.1084/jem.139.5.1228. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Grossberg S. E. The interferons and their inducers: molecular and therapeutic considerations. 3. N Engl J Med. 1972 Jul 20;287(3):122–128. doi: 10.1056/NEJM197207202870305. [DOI] [PubMed] [Google Scholar]
  10. Hoff R. L., Frenkel J. K. Cell-mediated immunity against Besnoitia and toxoplasma in specifically and cross-immunized hamsters and in cultures. J Exp Med. 1974 Mar 1;139(3):560–580. doi: 10.1084/jem.139.3.560. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Jondal M., Holm G., Wigzell H. Surface markers on human T and B lymphocytes. I. A large population of lymphocytes forming nonimmune rosettes with sheep red blood cells. J Exp Med. 1972 Aug 1;136(2):207–215. doi: 10.1084/jem.136.2.207. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Jones T. C., Hirsch J. G. The interaction between Toxoplasma gondii and mammalian cells. II. The absence of lysosomal fusion with phagocytic vacuoles containing living parasites. J Exp Med. 1972 Nov 1;136(5):1173–1194. doi: 10.1084/jem.136.5.1173. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Jones T. C. Macrophages and intracellular parasitism. J Reticuloendothel Soc. 1974 May;15(5):439–450. [PubMed] [Google Scholar]
  14. Jones T. C., Yeh S., Hirsch J. G. The interaction between Toxoplasma gondii and mammalian cells. I. Mechanism of entry and intracellular fate of the parasite. J Exp Med. 1972 Nov 1;136(5):1157–1172. doi: 10.1084/jem.136.5.1157. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. KAUFMAN H. E., MALONEY E. D. Multiplication of three strains of Toxoplasma gondii in tissue culture. J Parasitol. 1962 Jun;48:358–361. [PubMed] [Google Scholar]
  16. Kimball A. C., Kean B. H., Fuchs F. The role of toxoplasmosis in abortion. Am J Obstet Gynecol. 1971 Sep 15;111(2):219–226. doi: 10.1016/0002-9378(71)90893-3. [DOI] [PubMed] [Google Scholar]
  17. Koller C. A., King G. W., Hurtubise P. E., Sagone A. L., LoBuglio A. F. Characterization of glass adherent human mononuclear cells. J Immunol. 1973 Nov;111(5):1610–1612. [PubMed] [Google Scholar]
  18. Krahenbuhl J. L., Gaines J. D., Remington J. S. Lymphocyte transformation in human toxoplasmosis. J Infect Dis. 1972 Mar;125(3):283–288. doi: 10.1093/infdis/125.3.283. [DOI] [PubMed] [Google Scholar]
  19. Krahenbuhl J. L., Remington J. S. In vitro induction of nonspecific resistance in macrophages by specifically sensitized lymphocytes. Infect Immun. 1971 Oct;4(4):337–343. doi: 10.1128/iai.4.4.337-343.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Krahenbuhl J. L., Rosenberg L. T., Remington J. S. The role of thymus-derived lymphocytes in the in vitro activation of macrophages to kill Listeria monocytogenes. J Immunol. 1973 Oct;111(4):992–995. [PubMed] [Google Scholar]
  21. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  22. Lane F. C., Unanue E. R. Requirement of thymus (T) lymphocytes for resistance to listeriosis. J Exp Med. 1972 May 1;135(5):1104–1112. doi: 10.1084/jem.135.5.1104. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Mackaness G. B. Resistance to intracellular infection. J Infect Dis. 1971 Apr;123(4):439–445. doi: 10.1093/infdis/123.4.439. [DOI] [PubMed] [Google Scholar]
  24. Nathan C. F., Karnovsky M. L., David J. R. Alterations of macrophage functions by mediators from lymphocytes. J Exp Med. 1971 Jun 1;133(6):1356–1376. doi: 10.1084/jem.133.6.1356. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Nathan C. F., Remold H. G., David J. R. Characterization of a lymphocyte factor which alters macrophage functions. J Exp Med. 1973 Feb 1;137(2):275–290. doi: 10.1084/jem.137.2.275. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Patterson R. J., Youmans G. P. Demonstration in tissue culture of lymphocyte-mediated immunity to tuberculosis. Infect Immun. 1970 Jun;1(6):600–603. doi: 10.1128/iai.1.6.600-603.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Remington J. S., Krahenbuhl J. L., Mendenhall J. W. A role for activated macrophages in resistance to infection with Toxoplasma. Infect Immun. 1972 Nov;6(5):829–834. doi: 10.1128/iai.6.5.829-834.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Remington J. S. Toxoplasmosis: recent developments. Annu Rev Med. 1970;21:201–218. doi: 10.1146/annurev.me.21.020170.001221. [DOI] [PubMed] [Google Scholar]
  29. Rocklin R. E., Remold H. G., David J. R. Characterization of human migration inhibitory factor (MIF) from antigen-stimulated lymphocytes. Cell Immunol. 1972 Nov;5(3):436–445. doi: 10.1016/0008-8749(72)90070-6. [DOI] [PubMed] [Google Scholar]
  30. Rocklin R. E., Winston C. T., David J. R. Activation of human blood monocytes by products of sensitized lymphocytes. J Clin Invest. 1974 Feb;53(2):559–564. doi: 10.1172/JCI107590. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Rocklin R. E., Winston C. T., David J. R. Activation of human blood monocytes by products of sensitized lymphocytes. J Clin Invest. 1974 Feb;53(2):559–564. doi: 10.1172/JCI107590. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. SUTER E., RAMSEIAR H. CELLULAR REACTIONS IN INFECTION. Adv Immunol. 1964;27:117–173. doi: 10.1016/s0065-2776(08)60707-5. [DOI] [PubMed] [Google Scholar]
  33. Schmidt M. E., Douglas S. D., Rubin A. D. Human monocyte activation by supernatants from concanavalin A (con A) stimulated lymphocytes. Cell Immunol. 1973 Oct;9(1):45–59. doi: 10.1016/0008-8749(73)90166-4. [DOI] [PubMed] [Google Scholar]
  34. Simon H. B., Sheagren J. N. Cellular immunity in vitro. I. Immunologically mediated enhancement of macrophage bactericidal capacity. J Exp Med. 1971 Jun 1;133(6):1377–1389. doi: 10.1084/jem.133.6.1377. [DOI] [PMC free article] [PubMed] [Google Scholar]
  35. Simon H. B., Sheagren J. N. Enhancement of macrophage bactericidal capacity by antigenically stimulated immune lymphocytes. Cell Immunol. 1972 Jun;4(2):163–174. doi: 10.1016/0008-8749(72)90015-9. [DOI] [PubMed] [Google Scholar]
  36. Tizard I. R. Macrophage-cytophilic antibodies and the functions of macrophage-bound immunoglobulins. Bacteriol Rev. 1971 Dec;35(4):365–378. doi: 10.1128/br.35.4.365-378.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  37. Weiner M. S., Bianco C., Nussenzweig V. Enhanced binding of neuraminidase-treated sheep erythrocytes to human T lymphocytes. Blood. 1973 Dec;42(6):939–946. [PubMed] [Google Scholar]
  38. Weksler M. E., Hütteroth T. H. Impaired lymphocyte function in aged humans. J Clin Invest. 1974 Jan;53(1):99–104. doi: 10.1172/JCI107565. [DOI] [PMC free article] [PubMed] [Google Scholar]

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